Effect of electron transfer inhibitors on superoxide generation in the cytochrome bc1 site of the mitochondrial respiratory chain.

abstract：:HMr alpha 2CPI was found to be an inhibitor of human liver cathepsin H by the measurement of the dissociation constant (Ki), the association rate constant (k1) and the dissociation rate constant (k-1) between the enzyme and the inhibitor. These data suggest that this protein-proteinase inhibitor can play a physiologic...

journal_title：FEBS letters

authors： Pagano M,Engler R

更新日期：1984-01-23 00:00:00

abstract：:Mature dermal glands of Xenopus laevis contain storage granules with a characteristic ellipsoid shape. A few major proteins are present in these granules, including two with the same amino-terminal sequence and apparent molecular masses of 26 and 28 kDa. Using antibodies against these proteins, positive clones were is...

journal_title：FEBS letters

authors： Berger H,Kreil G

更新日期：1989-06-05 00:00:00

abstract：:Autophagy is a bulk degradation process conserved among eukaryotes. In macro-autophagy, autophagosomes sequester cytoplasmic components and deliver their contents to lysosomes/vacuoles. Autophagosome formation requires the conjugation of Atg8, a ubiquitin-like protein, to phosphatidylethanolamine (PE). Here we report ...

journal_title：FEBS letters

authors： Hanada T,Satomi Y,Takao T,Ohsumi Y

更新日期：2009-04-02 00:00:00

abstract：:Ischemia/reperfusion induces nuclear factor kappaB (NF-kappaB) and AP-1 in rat hearts after 15 min of ischemia followed by reperfusion (R) for various periods of time (15 and 30 min, 1, 2, 3, 6, 12, and 24 h). Low levels of NF-kappaB and no signal for AP-1 were detected in shams and in non-ischemic tissue distant from...

journal_title：FEBS letters

authors： Chandrasekar B,Freeman GL

更新日期：1997-01-13 00:00:00

abstract：:Low density lipoproteins are highly sensitive to oxidation by copper salts, and such peroxidation is accompanied by macrophage scavenger receptor recognition. This study shows that fresh human atherosclerotic material (aneurysms and endarterectomies) can contain detectable amounts of redox active iron and copper that ...

journal_title：FEBS letters

authors： Swain J,Gutteridge JM

更新日期：1995-07-24 00:00:00

abstract：:Transcriptional responses to growth in high environmental calcium concentrations were characterized and compared between wild-type and mutant Arabidopsis plants containing a knockout mutation in the gene encoding a cyclic nucleotide-gated channel (CNGC2). We show that the transcriptional profile of cngc2 plants grown ...

journal_title：FEBS letters

authors： Chan CW,Wohlbach DJ,Rodesch MJ,Sussman MR

更新日期：2008-03-19 00:00:00

abstract：:14-3-3s predominantly form homo-/heterodimers that are in equilibrium with corresponding monomers. Dimer/monomer equilibrium depends on the nature and phosphorylation of Ser58 of certain 14-3-3 isoforms. The structure and properties of 14-3-3 dimers are well characterized, whereas 14-3-3 monomers are less investigated...

journal_title：FEBS letters

authors： Sluchanko NN,Gusev NB

更新日期：2012-12-14 00:00:00

abstract：:The relationship between active transport of lactose via the lactose permease and the protonmotive force has been determined in E. coli cells using either the respiratory chain inhibitor cyanide or protonophores to decrease the protonmotive force progressively. In contradiction with the prediction of the delocalized c...

journal_title：FEBS letters

authors： Ghazi A,Delamourd L,Shechter E

更新日期：1986-12-15 00:00:00

abstract：:We report that sodium butyrate, a natural product of fiber degradation by colonic bacteria, markedly suppresses c-Myc-mediated apoptosis induced in murine plasmacytomas and human Burkitt lymphomas by growth factor deprivation, but not in cell lines devoid of c-myc translocations. Attenuation of cell death is associate...

journal_title：FEBS letters

authors： Alexandrov I,Romanova L,Mushinski F,Nordan R

更新日期：1998-08-28 00:00:00

abstract：:Thrombomodulin (TM) is a single-transmembrane glycoprotein receptor for thrombin, which is best known as a cofactor for thrombin-mediated activation of anticoagulant protein C. C-type lectin-like domain (CTLD) of TM has distinct coagulation/fibrinolysis-independent anti-inflammatory properties. Here we found anti-infl...

journal_title：FEBS letters

authors： Jin H,Yang X,Liu K,Gu Q,Xu X

更新日期：2011-11-04 00:00:00

abstract：:We have studied the perfusion of the jejunum and ileum in an isolated rat intestine model with flavonoids and hydroxycinnamates and the influence of glycosylation on the subsequent metabolism. Flavone and flavonol glucosides and their corresponding aglycones are glucuronidated during transfer across the rat jejunum an...

journal_title：FEBS letters

authors： Spencer JP,Chowrimootoo G,Choudhury R,Debnam ES,Srai SK,Rice-Evans C

更新日期：1999-09-17 00:00:00

abstract：:Galectin-3, with a wide tissue distribution and marked developmental regulation, provides significant insights into the progression of various disease and developmental stages. Recognized by its specificity for galactose, a detailed characterization of its sugar binding ability has been investigated by isothermal titr...

journal_title：FEBS letters

authors： Bachhawat-Sikder K,Thomas CJ,Surolia A

更新日期：2001-06-29 00:00:00

abstract：:Cytosolic NADP(+)-dependent malic enzyme (ME) from human tumor cells was characterized in detail and compared to ME from normal human tissues produced in recombinant E. coli. Kinetic properties, size as seen in SDS gels, and HPLC elution profiles of tryptic digests of human 'normal cell' ME and NADP(+)-ME from tumor c...

journal_title：FEBS letters

authors： Loeber G,Dworkin MB,Infante A,Ahorn H

更新日期：1994-05-16 00:00:00

abstract：:The stoichiometric complex formed between human leukocyte elastase and a synthetic MeO-Suc-Ala-Ala-Pro-Val chloromethyl ketone inhibitor was co-crystallized and its X-ray structure determined, using Patterson search methods. Its structure has been crystallographically refined to a final R value of 0.145 (8.0 and 2.3 A...

journal_title：FEBS letters

authors： Wei AZ,Mayr I,Bode W

更新日期：1988-07-18 00:00:00

abstract：:Monoamine oxidase B (MAO B) is an outer mitochondrial membrane protein that oxidizes arylalkylamine neurotransmitters and has been a valuable drug target for many neurological disorders. The 1.7 angstrom resolution structure of human MAO B shows the enzyme is dimeric with a C-terminal transmembrane helix protruding fr...

journal_title：FEBS letters

authors： Binda C,Hubálek F,Li M,Edmondson DE,Mattevi A

更新日期：2004-04-30 00:00:00

abstract：:The pre-steady state process in the thermolysin-catalyzed hydrolysis of Cbz-Gly-Phe-Ala was observed at pH 4.5 by fluorescence stopped-flow method using Dns-Phe as a displacement probe. After the confirmation of the pre-equilibrium hypothesis for the binary interaction, the nonlinear substrate concentration dependence...

journal_title：FEBS letters

authors： Kunugi S,Yokoyama M,Sakamoto A

更新日期：1995-04-03 00:00:00

abstract：:The N-terminal processing of MADH from the bacterium T. versutus and the N-terminal heterogeneity of the isolated alpha subunit of the alpha 2 beta 2 protein complex was demonstrated by a combination of Edman sequence analysis of an electroblotted band, in situ digested with pyroglutamate aminopeptidase, and accurate ...

journal_title：FEBS letters

authors： Van Beeumen J,Van Driessche G,Huitema F,Duine JA,Canters GW

更新日期：1993-10-25 00:00:00

abstract：:A central step in xanthone biosynthesis is the formation of the C13 skeleton, i.e. an intermediate benzophenone. Biosynthesis of 2,3',4,6-tetrahydroxybenzophenone from m-hydroxybenzoyl-CoA and malonyl-CoA was shown in cell-free extracts from cultured cells of Centaurium erythraea. The enzyme catalyzing this reaction w...

journal_title：FEBS letters

authors： Beerhues L

更新日期：1996-04-01 00:00:00

abstract：:The failure of insulin-regulated recruitment of the GLUT4 glucose transporter in cardiac muscle of obese Zucker rats is associated with alterations of the subcellular distribution of the small-molecular-mass GTP-binding protein rab4A. Here, we show by subcellular fractionation and Western blotting a translocation of t...

journal_title：FEBS letters

authors： Uphues I,Chern Y,Eckel J

更新日期：1995-12-18 00:00:00

abstract：:Alterations in both calcitonin (CT) secretion and plasma calcium were recently described in adult obese Zucker rats. We have investigated the CT biosynthetic activity of thyroid glands in 30-day-old obese Zucker rats (fa/fa), and their controls (Lean). Plasma calcium level was significantly increased (+0.6 mg/dl) in o...

journal_title：FEBS letters

authors： Segond N,Jullienne A,Tahri EH,Garel JM

更新日期：1984-08-20 00:00:00

abstract：:The antibacterial activity of many small, positively charged peptides and proteins is based on pore formation in lipid bilayers. It is here proposed to arise from an electroporation effect. This hypothesis is supported by calculations of the electrostatic potential of NK-lysin associated to a membrane. For a significa...

journal_title：FEBS letters

authors： Miteva M,Andersson M,Karshikoff A,Otting G

更新日期：1999-11-26 00:00:00

abstract：:Biological functions of transcribed spacer regions in eucaryotic pre-rRNAs remain elusive so far. Utilization of the comparative approach to analyse their secondary structure has been impeded by the extensive sequence divergence observed among most of the specimens available to date. However, we have taken advantage o...

journal_title：FEBS letters

authors： Michot B,Despres L,Bonhomme F,Bachellerie JP

更新日期：1993-02-01 00:00:00

abstract：:Stimulation of mas-oncogene transfected 401L-C3 cells by angiotensins leads to the production of inositol phosphates. This response shows dose dependence, and has an apparent rank order of potency angiotensin III greater than or equal to angiotensin II much greater than angiotensin I. Preincubation with 12-O-tetradeca...

journal_title：FEBS letters

authors： Jackson TR,Hanley MR

更新日期：1989-07-17 00:00:00

abstract：:The effects of synthetic rat adrenomedullin (rAM), a novel vasorelaxant peptide originally isolated from human pheochromocytoma, on receptor binding and cAMP generation were studied in cultured rat vascular smooth muscle cells (VSMC). A binding study using [125I]rAM revealed the presence of a single class of high-affi...

journal_title：FEBS letters

authors： Eguchi S,Hirata Y,Kano H,Sato K,Watanabe Y,Watanabe TX,Nakajima K,Sakakibara S,Marumo F

更新日期：1994-03-07 00:00:00

abstract：:The tendency of DNA to form fibers upon condensation with counterions is reviewed. It is shown that chromatin fibers may acquire a relatively constant diameter of about 30 nm simply as an optimal size achieved upon neutralization of DNA, without requiring a repetitive internal structure. Thus the size of chromatin fib...

journal_title：FEBS letters

authors： Subirana JA

更新日期：1992-05-11 00:00:00

abstract：:gamma-Thrombin stimulated release of [3H]arachidonic acid ([3H]AA) accompanied by a significant production of PAF and lyso-PAF by rabbit platelets. These responses, which reflect PLA2 activation, were observed after a prolonged lag and to a lower extent when compared to those induced by alpha-thrombin which evoked a m...

journal_title：FEBS letters

authors： Touqui L,Jandrot-Perrus M,Vargaftig BB

更新日期：1989-09-25 00:00:00

abstract：:Using a prostate-specific antigen cDNA as a hybridization probe, clones containing the kallikrein genes encoding prostate-specific antigen, human glandular kallikrein-1 and pancreas/kidney kallikrein were isolated from a human genomic library. Clones containing the prostate-specific antigen gene and the human glandula...

journal_title：FEBS letters

authors： Riegman PH,Vlietstra RJ,Klaassen P,van der Korput JA,Geurts van Kessel A,Romijn JC,Trapman J

更新日期：1989-04-10 00:00:00

abstract：:Ataxin-3, which is encoded by a gene that has been associated with Machado-Joseph disease, contains a catalytic N-terminal Josephin domain with deubiquitinase activity. Here, we show that the Josephin domain of ataxin 3 catalyzes endo-type cleavage of Lys48-linked polyubiquitin. Furthermore, NMR data obtained followin...

journal_title：FEBS letters

authors： Satoh T,Sumiyoshi A,Yagi-Utsumi M,Sakata E,Sasakawa H,Kurimoto E,Yamaguchi Y,Li W,Joazeiro CA,Hirokawa T,Kato K

更新日期：2014-11-28 00:00:00

abstract：:A pumpkin 2S albumin with ribonuclease (RNase) activity was purified from pumpkin seeds (Cucurbita sp.) by liquid chromatographic techniques. It manifested potent RNase activity toward baker's yeast RNA and calf liver RNA, and some polyhomoribonucleotides, including poly(A), poly(U) and poly(C) but not poly(G). Moreov...

journal_title：FEBS letters

authors： Fang EF,Wong JH,Lin P,Ng TB

更新日期：2010-09-24 00:00:00

abstract：:KOR-3, also known as ORL-1, is a member of the opioid receptor family, encoding the murine receptor for orphanin FQ/nociceptin. In the current studies we have identified five different splice variants of KOR-3 in mouse brain, three of which have not been previously reported. In addition to variants with a 15 bp deleti...

journal_title：FEBS letters

authors： Pan YX,Xu J,Wan BL,Zuckerman A,Pasternak GW

更新日期：1998-09-11 00:00:00