Abstract:
:Cytosolic NADP(+)-dependent malic enzyme (ME) from human tumor cells was characterized in detail and compared to ME from normal human tissues produced in recombinant E. coli. Kinetic properties, size as seen in SDS gels, and HPLC elution profiles of tryptic digests of human 'normal cell' ME and NADP(+)-ME from tumor cells were identical. Thus, NADP(+)-ME found in tumor cells does not constitute a tumor-specific isoform as suggested by other studies but is identical to the 'housekeeping protein' predominantly expressed in human liver and white adipose tissue.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Loeber G,Dworkin MB,Infante A,Ahorn Hdoi
10.1016/0014-5793(94)00386-6subject
Has Abstractpub_date
1994-05-16 00:00:00pages
181-6issue
2-3eissn
0014-5793issn
1873-3468pii
0014-5793(94)00386-6journal_volume
344pub_type
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