The prostate-specific antigen gene and the human glandular kallikrein-1 gene are tandemly located on chromosome 19.

abstract：:Superoxide reacts with nitric oxide to form peroxynitrite, a potent oxidising agent which may contribute to tissue damage in pathological situations such as inflammation and ischaemia/reperfusion. One mechanism by which oxidative stress damages tissues is the induction of a specific Cyclosporin A-sensitive mitochondri...

journal_title：FEBS letters

authors： Packer MA,Murphy MP

更新日期：1994-05-30 00:00:00

abstract：:The structure of the Ala38 variant of yeast iso-1-cytochrome c, in which the previously unchanged Arg38 has been replaced, has been characterised by NMR. The NMR data indicate that the structure of the Ala38 variant is very similar to that of the wild type protein. In particular, the heme environment and interactions ...

journal_title：FEBS letters

authors： Thurgood AG,Pielak GJ,Cutler RL,Davies AM,Greenwood C,Mauk AG,Smith M,Williamson DJ,Moore GR

更新日期：1991-06-24 00:00:00

abstract：:Small RNAs (miR159-like RNAs) identical to some plant miR159s were found in Xenopus tropicalis miRNA cDNA libraries (30 miRNA families consisting of 75 unique sequences). Preferential expression of this RNA species was found in neural tissues during development. A sequence matching to this RNA species was not found in...

journal_title：FEBS letters

authors： Hikosaka A,Takaya K,Jinno M,Kawahara A

更新日期：2007-06-26 00:00:00

abstract：:Malonyl-CoA-acyl carrier protein transacylase (MCAT) transfers the malonyl group from malonyl-CoA to holo-acyl carrier protein (ACP), and since malonyl-ACP is a key building block for fatty-acid biosynthesis it is considered as a promising antibacterial target. The crystal structures of MCAT from Staphylococcus aureus...

journal_title：FEBS letters

authors： Hong SK,Kim KH,Park JK,Jeong KW,Kim Y,Kim EE

更新日期：2010-03-19 00:00:00

abstract：:Under conditions of molar excess of enzyme, isolated F1-ATPase from beef heart mitochondria catalyses ATP hydrolysis biphasically. The rate constants for product release are approximately 10(-1) and 10(-4)-10(-3) s-1, respectively. The slow phase of ATP hydrolysis is insensitive to EDTA. [gamma-32P]ATP splitting in th...

journal_title：FEBS letters

authors： Milgrom YaM,Murataliev MB

更新日期：1987-09-28 00:00:00

abstract：:Turnip yellow mosaic virus (TYMV) is a positive-strand RNA virus able to infect Arabidopsis thaliana. To establish a TYMV infection system in Arabidopsis cell culture, TYMV replicons with the capsid protein gene replaced by a reporter gene expressing the Sh ble protein conferring zeocin resistance were used to transfe...

journal_title：FEBS letters

authors： Camborde L,Tournier V,Noizet M,Jupin I

更新日期：2007-01-23 00:00:00

abstract：:A rapidly maturing variant of the red fluorescent protein DsRed was optimized for bacterial expression by random mutagenesis. The brightest variant contains six mutations, two of which (S4T and a silent mutation in codon 2) explain most of the fluorescence enhancement. The novel variants are expressed at 9-60-fold hig...

journal_title：FEBS letters

authors： Sörensen M,Lippuner C,Kaiser T,Misslitz A,Aebischer T,Bumann D

更新日期：2003-09-25 00:00:00

abstract：:Mitogen-activated protein (MAP) kinases mediate cellular responses to a wide variety of stimuli. Activation of a MAP kinase occurs after phosphorylation by an upstream dual-specificity protein kinase, known as a MAP kinase kinase or MEK. The Arabidopsis thaliana genome encodes 10 MEKs but few of these have been shown ...

journal_title：FEBS letters

authors： Melikant B,Giuliani C,Halbmayer-Watzina S,Limmongkon A,Heberle-Bors E,Wilson C

更新日期：2004-10-08 00:00:00

abstract：:The structure determination of apotransketolase and the comparison of its three-dimensional structure with that of the holoenzyme has revealed that no large conformational changes are associated with cofactor binding. Two loops at the active site are flexible in the apoenzyme which enables ThDP to reach its binding si...

journal_title：FEBS letters

authors： Sundström M,Lindqvist Y,Schneider G

更新日期：1992-11-30 00:00:00

abstract：:The murine mammary FM3A/O and the thymidylate (dTMP) synthetase-deficient FM3A/TS- carcinoma cell lines can be considered as a novel and useful test system for the detection of nucleoside analogues which are directly aimed at the thymidylate synthetase. These compounds should be inhibitory for FM3A/O but not for FM3A/...

journal_title：FEBS letters

authors： Balzarini J,de Clercq E,Ayusawa D,Seno T

更新日期：1984-07-23 00:00:00

abstract：:The farnesoid X receptor (FXR) is a nuclear receptor activated by bile acids (BAs). In response to ligand-binding, FXR regulates many genes involved in BA, lipid, and lipoprotein metabolism. To identify new FXR target genes, microarray technology was used to profile total RNA extracted from HepG2 cells treated with th...

journal_title：FEBS letters

authors： Sirvent A,Claudel T,Martin G,Brozek J,Kosykh V,Darteil R,Hum DW,Fruchart JC,Staels B

更新日期：2004-05-21 00:00:00

abstract：:Nuclear magnetic resonance (NMR) spectroscopy was used to determine the secondary structure and the three-dimensional polypeptide backbone fold of the human sterol carrier protein 2 (hSCP2), which is a basic protein with 123 residues believed to participate in the intracellular transport of cholesterol and various oth...

journal_title：FEBS letters

authors： Szyperski T,Scheek S,Johansson J,Assmann G,Seedorf U,Wüthrich K

更新日期：1993-11-29 00:00:00

abstract：:The X-ray crystal structures of the complexes formed with bovine trypsin and the N alpha-tosylated piperidides of m-amidino-, p-amidino- and p-guanidino-D,L-phenylalanine (3-TAPAP, 4-TAPAP and 4-TGPAP) were determined with data to 1.8 A resolution. The L-stereoisomer of 3-TAPAP binds as a compact entity into the activ...

journal_title：FEBS letters

authors： Turk D,Stürzebecher J,Bode W

更新日期：1991-08-05 00:00:00

abstract：:Rab proteins are small GTPases involved in the regulation of membrane traffic. Rab5a has been shown to regulate transport in the early endocytic pathway. Here we report the isolation of cDNA clones encoding two highly related isoforms, Rab5b and Rab5c. The two proteins share with Rab5a all the structural features requ...

journal_title：FEBS letters

authors： Bucci C,Lütcke A,Steele-Mortimer O,Olkkonen VM,Dupree P,Chiariello M,Bruni CB,Simons K,Zerial M

更新日期：1995-06-05 00:00:00

abstract：:The WW domain is known as one of the smallest protein modules with a triple-stranded beta-sheet fold. Here, we present the solution structure of the second WW domain from the mouse salvador homolog 1 protein. This WW domain forms a homodimer with a beta-clam-like motif, as evidenced by size exclusion chromatography, a...

journal_title：FEBS letters

authors： Ohnishi S,Güntert P,Koshiba S,Tomizawa T,Akasaka R,Tochio N,Sato M,Inoue M,Harada T,Watanabe S,Tanaka A,Shirouzu M,Kigawa T,Yokoyama S

更新日期：2007-02-06 00:00:00

abstract：:Recombinant adipogenesis inhibitory factor (AGIF) was purified to homogeneity from the conditioned medium of COS-1 cells transfected with human AGIF cDNA. The amino-terminal sequence analysis of the mature AGIF revealed that AGIF was produced as a precursor consisting of 199 amino acids and processed into a mature for...

journal_title：FEBS letters

authors： Ohsumi J,Miyadai K,Kawashima I,Ishikawa-Ohsumi H,Sakakibara S,Mita-Honjo K,Takiguchi Y

更新日期：1991-08-19 00:00:00

abstract：:Pheromone receptors are expressed in the accessory olfactory system, which is vital for non-specific chemical communication and for sexual behavior. Under the hypothesis that some of the pheromone molecules released from female reproductive organs might regulate sperm chemotaxis or chemokinesis, we examined whether th...

journal_title：FEBS letters

authors： Tatsura H,Nagao H,Tamada A,Sasaki S,Kohri K,Mori K

更新日期：2001-01-19 00:00:00

abstract：:The cellulases cellobiohydrolase 1 (CBH 1) and endoglucanase 1 (EG 1) from the fungus Trichoderma reesei are closely related with 40% sequence identity and very similar in structure. In CBH 1 the active site is enclosed by long loops and some antiparallel beta-strands forming a 40 A long tunnel, whereas in EG 1 part o...

journal_title：FEBS letters

authors： Henriksson H,Ståhlberg J,Isaksson R,Pettersson G

更新日期：1996-07-29 00:00:00

abstract：:To investigate the biochemical basis of the HIV-1 resistance to AZT we obtained the RT mutant containing four amino acid substitutions by an oligonucleotide-directed mutagenesis technique. Enzymatic properties of the wild type and mutant RTs were compared. 'AZT-resistant' mutations in RT were shown to be associated wi...

journal_title：FEBS letters

authors： Pokholok DK,Gudima SO,Yesipov DS,Dobrynin VN,Rechinsky VO,Kochetkov SN

更新日期：1993-07-05 00:00:00

abstract：:The heterotrophic nitrifier Paracoccus denitrificans expresses a membrane-associated ammonia monooxygenase. The active enzyme has been solubilized in the detergent dodecyl-beta-D-maltoside and purified by standard chromatographic techniques. This is the first purification of an ammonia monooxygenase. The enzyme consis...

journal_title：FEBS letters

authors： Moir JW,Crossman LC,Spiro S,Richardson DJ

更新日期：1996-05-27 00:00:00

abstract：:The non-ionic detergent Brij 58P is recommended as a stabilizing agent for protein storage; for example, the aggregation-prone chaperone DnaJ can be maintained in solution by low concentrations of Brij 58P. During protein folding studies with alpha-glucosidase, rhodanese and citrate synthase as model proteins, we disc...

journal_title：FEBS letters

authors： Krause M,Rudolph R,Schwarz E

更新日期：2002-12-04 00:00:00

abstract：:The carbohydrate chains of the pathological human immunoglobulins M from two patients with Waldenström's macroglobulinemia were released by hydrazinolysis. The N-acetyllactosamine-type glycans were obtained by affinity chromatography on concanavalin A and fractionated by high-voltage paper electrophoresis. The primary...

journal_title：FEBS letters

authors： Cahour A,Debeire P,Hartmann L,Montreuil J,van Halbeek H,Vliegenthart JF

更新日期：1984-05-21 00:00:00

abstract：:Absence through mutation of two proteins involved in periplasmic disulphide bond formation, DsbA and DsbB, results in failure of anaerobically grown Escherichia coli to synthesise the holo forms of either its endogenous c-type cytochrome nitrite reductase or exogenous cytochrome c550 from Paracoccus denitrificans. The...

journal_title：FEBS letters

authors： Sambongi Y,Ferguson SJ

更新日期：1996-12-02 00:00:00

abstract：:The solution conformation of the cardiac stimulatory sea anemone polypeptide anthopleurin-A has been characterised using distance geometry and restrained molecular dynamics calculations. A set of 253 approximate interproton distance restraints and 14 peptide backbone torsion angle restraints derived from two-dimension...

journal_title：FEBS letters

authors： Torda AE,Mabbutt BC,van Gunsteren WF,Norton RS

更新日期：1988-11-07 00:00:00

abstract：:We have previously shown that the Mycoplasma mycoides glycine tRNA (anticodon UCC) effectively reads the codons GGU and GGC in violation of the classic codon reading rules. We have attempted to elucidate what structural elements in this tRNA molecule confer this translational property and in the course of this investi...

journal_title：FEBS letters

authors： Claesson C,Samuelsson T,Lustig F,Borén T

更新日期：1990-10-29 00:00:00

abstract：:The expression of rat brain voltage-sensitive Na+ channel mRNAs in Schwann cells was examined using in situ hybridization cytochemistry and RT-PCR. The mRNAs of rat brain Na+ channel subtype II and III, but not subtype I, were detected in cultured Schwann cells from sciatic nerve and in intact sciatic nerve, which con...

journal_title：FEBS letters

authors： Oh Y,Black JA,Waxman SG

更新日期：1994-08-22 00:00:00

abstract：:A 1000-bp fragment of Enterococcus hirae genomic DNA was amplified by the polymerase chain reaction method, using the oligonucleotide primers designed from amino acid sequences of both amino-terminal and a tryptic fragment of the Na(+)-ATPase alpha-subunit in this organism. DNA sequencing of this product revealed that...

journal_title：FEBS letters

authors： Kakinuma Y,Igarashi K,Konishi K,Yamato I

更新日期：1991-11-04 00:00:00

abstract：:We have used DNase I footprinting to examine the effect of a novel naphthylquinoline dimer, designed as a triplex-specific bis-intercalator, on the stability of intermolecular DNA triplexes. We find that this compound efficiently promotes triplex formation between the 9-mer oligonucleotide 5'-TTTTTTCTT and its oligopu...

journal_title：FEBS letters

authors： Keppler M,Zegrocka O,Strekowski L,Fox KR

更新日期：1999-03-26 00:00:00

abstract：:The amyloid precursor protein (APP) is proteolytically processed by β- and γ-secretases to release amyloid-β peptide (Aβ), the main component found in senile plaques of Alzheimer's disease (AD) patient brains. Alternatively, APP can be cleaved within the Aβ sequence by α-secretase, thus precluding the generation of Aβ...

journal_title：FEBS letters

authors： León-Otegui M,Gómez-Villafuertes R,Díaz-Hernández JI,Díaz-Hernández M,Miras-Portugal MT,Gualix J

更新日期：2011-07-21 00:00:00

abstract：:The mammalian Ptdlns 3-kinase is shown to be inhibited by low nanomolar concentrations of demethoxyviridin, an antifungal agent structurally related to wortmannin. The inhibitory potency of both compounds could be observed in purified Ptdlns 3-kinase whether or not the regulatory subunit (p85 alpha) was present, sugge...

journal_title：FEBS letters

authors： Woscholski R,Kodaki T,McKinnon M,Waterfield MD,Parker PJ

更新日期：1994-04-04 00:00:00