Abstract:
:To investigate the biochemical basis of the HIV-1 resistance to AZT we obtained the RT mutant containing four amino acid substitutions by an oligonucleotide-directed mutagenesis technique. Enzymatic properties of the wild type and mutant RTs were compared. 'AZT-resistant' mutations in RT were shown to be associated with the reduced capability of AZT-TP to block the DNA- but not RNA-directed DNA synthesis.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Pokholok DK,Gudima SO,Yesipov DS,Dobrynin VN,Rechinsky VO,Kochetkov SNdoi
10.1016/0014-5793(93)81080-jsubject
Has Abstractpub_date
1993-07-05 00:00:00pages
237-41issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(93)81080-Jjournal_volume
325pub_type
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