Abstract:
:A rapidly maturing variant of the red fluorescent protein DsRed was optimized for bacterial expression by random mutagenesis. The brightest variant contains six mutations, two of which (S4T and a silent mutation in codon 2) explain most of the fluorescence enhancement. The novel variants are expressed at 9-60-fold higher levels in Escherichia coli compared to DsRed.T3, but are not superior fluorophores on a per molecule basis. In contrast to previously available DsRed variants, DsRed.T3_S4T is sufficiently bright to monitor Salmonella gene expression in infected animals using flow cytometry. However, no fluorescence enhancement was observed in Leishmania or HeLa cells, indicating that these novel variants are specifically useful for bacteria.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sörensen M,Lippuner C,Kaiser T,Misslitz A,Aebischer T,Bumann Ddoi
10.1016/s0014-5793(03)00856-1keywords:
subject
Has Abstractpub_date
2003-09-25 00:00:00pages
110-4issue
2-3eissn
0014-5793issn
1873-3468pii
S0014579303008561journal_volume
552pub_type
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