Interaction between human amphipathic apolipoproteins and amyloid beta-peptide: surface plasmon resonance studies.


:Several apolipoproteins including apoE and apoA-I are known to be associated with amyloid beta-peptide, a major component of senile plaques in Alzheimer's disease. In the present study the interaction between three human amphipathic apolipoproteins apoE3, apoA-I and apoA-II and immobilized amyloid beta-peptide (1-40) was quantified by plasmon resonance. The interactions were saturable and reversible. The results demonstrated a high affinity of the binding of amphipathic apolipoproteins to amyloid beta-peptide. On the other hand, only a small population of synthetic amyloid beta-peptide participated in the interaction. The apparent equilibrium dissociation constants K(D) were 10 nM for apoE3, 25 nM for apoA-I and 80 nM for apoA-II under physiological conditions. The affinity of the apoE3-amyloid beta-peptide binding was not affected by pH in the range 6.0-8.0 but was significantly increased by high salt concentration. ApoA-I mainly followed similar patterns. A major participation of hydrophobic forces in the binding of apoE3 and apoA-I to amyloid beta-peptide was suggested.




FEBS letters


Shuvaev VV,Siest G




Has Abstract


1996-03-25 00:00:00














  • Solution NMR determination of hydrogen bonding and base pairing between the glyQS T box riboswitch Specifier domain and the anticodon loop of tRNA(Gly).

    abstract::In Gram-positive bacteria the tRNA-dependent T box riboswitch regulates the expression of many amino acid biosynthetic and aminoacyl-tRNA synthetase genes through a transcription attenuation mechanism. The Specifier domain of the T box riboswitch contains the Specifier sequence that is complementary to the tRNA antico...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Chang AT,Nikonowicz EP

    更新日期:2013-11-01 00:00:00

  • Translational regulations as specific traits of chloroplast gene expression.

    abstract::Studies of protein synthesis in the chloroplast compartment have revealed a unique combination of translational autoregulations and trans-regulations due to the delivery of a variety of nuclear factors that act post-transcriptionally. We show how these two characteristics concur to set the major step in the regulation...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Choquet Y,Wollman FA

    更新日期:2002-10-02 00:00:00

  • A photo-CIDNP study of the active sites of Megasphaera elsdenii and Clostridium MP flavodoxins.

    abstract::Megasphaera elsdenii and Clostridium MP flavodoxins have been investigated by photo-CIDNP techniques. Using time-resolved spectroscopy and external dyes carrying different charges it was possible to assign unambiguously the resonance lines in the NMR-spectra to tyrosine, tryptophan and methionine residues in the two p...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Moonen CT,Hore PJ,Müller F,Kaptein R,Mayhew SG

    更新日期:1982-11-22 00:00:00

  • Expression of c-fos gene inhibits proteoglycan synthesis in transfected chondrocyte.

    abstract::The effect of expression of c-fos gene on proteoglycan synthesis, one of the important markers of cartilage metabolism, was examined by introducing the c-fos DNA into HCS 2/8 chondrocytes. The [35S]sulfate incorporation into proteoglycan was decreased in the c-fos transfectants expressing exogenous c-fos mRNA, when co...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tsuji M,Funahashi S,Takigawa M,Seiki M,Fujii K,Yoshida T

    更新日期:1996-03-04 00:00:00

  • Dimerization, ubiquitylation and endocytosis go together in growth hormone receptor function.

    abstract::Internalization of membrane proteins has been studied for more than three decades without solving all the underlying mechanisms. Our knowledge of the clathrin-coated endocytosis is sufficient to understand the basic principles. However, more detailed insight is required to recognize why different proteins enter clathr...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Strous GJ,Gent J

    更新日期:2002-10-02 00:00:00

  • A comparison of demethoxyviridin and wortmannin as inhibitors of phosphatidylinositol 3-kinase.

    abstract::The mammalian Ptdlns 3-kinase is shown to be inhibited by low nanomolar concentrations of demethoxyviridin, an antifungal agent structurally related to wortmannin. The inhibitory potency of both compounds could be observed in purified Ptdlns 3-kinase whether or not the regulatory subunit (p85 alpha) was present, sugge...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Woscholski R,Kodaki T,McKinnon M,Waterfield MD,Parker PJ

    更新日期:1994-04-04 00:00:00

  • Phospholipid phase transitions in homogeneous nanometer scale bilayer discs.

    abstract::Nanoscale protein supported phospholipid bilayer discs, or Nanodiscs, were produced for the purpose of studying the phase transition behavior of the incorporated lipids. Nanodiscs and vesicles were prepared with two phospholipids, dipalmitoyl phosphatidylcholine and dimyristoyl phosphatidylcholine, and the phase trans...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Shaw AW,McLean MA,Sligar SG

    更新日期:2004-01-02 00:00:00

  • Pore mutations affecting tetrameric assembly and functioning of the potassium channel KcsA from Streptomyces lividans.

    abstract::Designed mutations within the Streptomyces lividans kcsA gene resulted in a set of mutant proteins, which were characterized in respect to their assembly and channel activities. (i) The amino acid residue leucine 81 located at the external side of KcsA was found to be exchangeable by a cysteine residue without affecti...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Splitt H,Meuser D,Borovok I,Betzler M,Schrempf H

    更新日期:2000-04-21 00:00:00

  • Proteins required for vacuolar function are targets of lysine polyphosphorylation in yeast.

    abstract::Polyphosphates (polyP) are long chains of inorganic phosphates that can be attached to lysine residues of target proteins as a nonenzymatic post-translational modification. This modification, termed polyphosphorylation, may be particularly prevalent in bacterial and fungal species that synthesize large quantities of p...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: McCarthy L,Bentley-DeSousa A,Denoncourt A,Tseng YC,Gabriel M,Downey M

    更新日期:2020-01-01 00:00:00

  • H(2)O(2) generation during the auto-oxidation of coniferyl alcohol drives the oxidase activity of a highly conserved class III peroxidase involved in lignin biosynthesis.

    abstract::Characterization of lignified Zinnia elegans hypocotyls by both alkaline nitrobenzene oxidation and thioacidolysis reveals that coniferyl alcohol units are mainly found as part of 4-O-linked end groups and aryl-glycerol-beta-aryl ether (beta-O-4) structures. Z. elegans hypocotyls also contain a basic peroxidase (EC 1....

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pomar F,Caballero N,Pedreño M,Ros Barceló A

    更新日期:2002-10-09 00:00:00

  • Crystal structure of the human tau PHF core domain VQIINK complexed with the Fab domain of monoclonal antibody Tau2r3.

    abstract::Neurofibrillary tangles formed by abnormally aggregated tau protein are a histopathological feature of tauopathies. A tau aggregation inhibitor is a potential therapeutic agent for tauopathies. In this study, we prepared a monoclonal antibody for tau, monoclonal antibody to tau protein (Tau2r3), using as epitope the 2...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Tsuchida T,Susa K,Kibiki T,Tsuchiya T,Miyamoto K,In Y,Minoura K,Taniguchi T,Ishida T,Tomoo K

    更新日期:2020-04-13 00:00:00

  • Involvement of MAPK signaling molecules and Runx2 in the NELL1-induced osteoblastic differentiation.

    abstract::NELL1 is an extracellular protein inducing osteogenic differentiation and bone formation of osteoblastic cells. To elucidate the intracellular signaling cascade evoked by NELL1, we have shown that NELL1 protein transiently activates the MAPK signaling cascade, induces the phosphorylation of Runx2, and promotes the rap...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bokui N,Otani T,Igarashi K,Kaku J,Oda M,Nagaoka T,Seno M,Tatematsu K,Okajima T,Matsuzaki T,Ting K,Tanizawa K,Kuroda S

    更新日期:2008-01-23 00:00:00

  • Pulmonary surfactant is a potential endogenous inhibitor of proteolytic activation of Sendai virus and influenza A virus.

    abstract::The pathogenicities of influenza viruses and paramyxoviruses have been proposed to be primarily determined by a host cell protease(s) that activates viral infectivity by proteolytic cleavage of the envelope glycoproteins. We recently isolated a trypsin-type endoprotease, named tryptase Clara, from rat bronchial and br...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kido H,Sakai K,Kishino Y,Tashiro M

    更新日期:1993-05-10 00:00:00

  • Nature of the pH-induced conformational changes and exposure of the C-terminal region of chromogranin A.

    abstract::Chromogranin A is known to undergo pH induced conformational changes, and the difference in conformation is supposed to be responsible for the difference in Ca2+ binding property. To gain insight regarding the overall structure and the nature of pH-induced conformational changes of chromogranin A, limited trypsin dige...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Yoo SH,Ferretti JA

    更新日期:1993-11-22 00:00:00

  • Effect of inhibitor binding to beta subunits of F1ATPase on enzyme thermostability: a kinetic and FT-IR spectroscopic analysis.

    abstract::FT-IR analysis shows that treatment of F1ATPase with the inhibitors DCCD and Nbf-Cl, in the presence of saturating concentrations of ADP and AMP-PNP and in the absence of Mg2+, does not modify the secondary structure of the enzyme, but significantly modifies its compactness and thermal stability, although to different...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lippe G,Tanfani F,Di Pancrazio F,Contessi S,Bertoli E,Dabbeni-Sala F

    更新日期:1998-08-07 00:00:00

  • The predicted molecular structure suggests that CTF/NF-I may function as a histone acetylase.

    abstract::The primary structure of nuclear factor-I (CTF/NF-I), a eukaryotic regulatory DNA-binding protein involved in both DNA replication and gene transcription, and the secondary structure predictable from it, are compared here with those of a number of prokaryotic acetylases. Hydropathy and Chou-Fasman analyses reveal that...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Oikarinen J,Mannermaa RM

    更新日期:1990-10-29 00:00:00

  • An inhibitory effect of actin on casein kinase II activity in vitro.

    abstract::The inhibitory effect of actin on protein phosphorylation by three distinct protein kinases (CK-II, A-kinase and MAP-kinase) was examined in vitro. It was found that: (i) actin inhibits the activities of alpha-monomeric CK-II (CK-IIalpha) as well as oligomeric CK-II (alpha2beta2) in a dose-dependent manner, but has no...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Karino A,Tanoue S,Fukuda M,Nakamura T,Ohtsuki K

    更新日期:1996-12-02 00:00:00

  • miR-1273g-3p modulates activation and apoptosis of hepatic stellate cells by directly targeting PTEN in HCV-related liver fibrosis.

    abstract::MicroRNA (miRNA) play a pivotal role in the development of liver fibrosis. However, the functions of miRNA in hepatitis C virus (HCV)-related liver fibrosis remain unclear. In this study, we systematically analyzed the microarray data of the serum miRNA in patients with HCV-induced hepatic fibrosis. Among 41 dysregula...

    journal_title:FEBS letters

    pub_type: 信件


    authors: Niu X,Fu N,Du J,Wang R,Wang Y,Zhao S,Du H,Wang B,Zhang Y,Sun D,Nan Y

    更新日期:2016-08-01 00:00:00

  • Bcl-2 and Bax are involved in the sympathetic protection of brown adipocytes from obesity-linked apoptosis.

    abstract::Obesity is linked to functional brown adipose tissue (BAT) atrophy, partially due to adipocyte apoptosis. The brown adipocytes of obese rats have lower Bcl-2/Bax mRNA and protein ratios than those of their lean littermates. Exposure to a low temperature for three days markedly increased the Bcl-2/Bax ratio, by increas...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Briscini L,Tonello C,Dioni L,Carruba MO,Nisoli E

    更新日期:1998-07-10 00:00:00

  • Leukotriene A4 hydrolase, a bifunctional enzyme. Distinction of leukotriene A4 hydrolase and aminopeptidase activities by site-directed mutagenesis at Glu-297.

    abstract::We previously obtained evidence for intrinsic aminopeptidase activity for leukotriene (LT)A4 hydrolase, an enzyme characterized to specifically catalyse the hydrolysis of LTA4 to LTB4, a chemotactic compound. From a sequence homology search between LTA4 hydrolase and several aminopeptidases, it became clear that they ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Minami M,Bito H,Ohishi N,Tsuge H,Miyano M,Mori M,Wada H,Mutoh H,Shimada S,Izumi T

    更新日期:1992-09-14 00:00:00

  • Structural organization of the bovine cathelicidin gene family and identification of a novel member.

    abstract::Cathelicidins are a group of myeloid antimicrobial peptide precursors found in a variety of mammalian species. Transcripts of this family show a highly conserved 5' region corresponding to the 5' untranslated region, signal peptide and propiece, and diverse 3' regions encoding structurally varied C-terminal sequences ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Scocchi M,Wang S,Zanetti M

    更新日期:1997-11-17 00:00:00

  • Ubiquitylation of DNA polymerase λ.

    abstract::DNA polymerase (pol) λ, one of the 15 cellular pols, belongs to the X family. It is a small 575 amino-acid protein containing a polymerase, a dRP-lyase, a proline/serine rich and a BRCT domain. Pol λ shows various enzymatic activities including DNA polymerization, terminal transferase and dRP-lyase. It has been implic...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Markkanen E,van Loon B,Ferrari E,Hübscher U

    更新日期:2011-09-16 00:00:00

  • 4-isoavenaciolide covalently binds and inhibits VHR, a dual-specificity phosphatase.

    abstract::A potent inhibitor of a dual-specificity protein phosphatase, VHR (vaccinia H1 related), was isolated during a screening of microbial metabolites. This inhibitor was identified as 4-isoavenaciolide (4-iA), and was determined to irreversibly inhibit VHR phosphatase activity with a 50% inhibitory concentration of 1.2 mi...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ueda K,Usui T,Nakayama H,Ueki M,Takio K,Ubukata M,Osada H

    更新日期:2002-08-14 00:00:00

  • Sulphur islands in the Escherichia coli genome: markers of the cell's architecture?

    abstract::Two highly contrasted images depict genomes: at first sight, genes appear to be distributed randomly along the chromosome. In contrast, their organisation into operons (or pathogenicity islands) suggests that, at least locally, related functions are in physical proximity. Analysis of the codon usage bias in orthologou...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Rocha EP,Sekowska A,Danchin A

    更新日期:2000-06-30 00:00:00

  • Farnesylcysteine analogues inhibit chemotactic peptide receptor-mediated G-protein activation in human HL-60 granulocyte membranes.

    abstract::Analogues of S-prenylated cysteine like N-acetyl-S-trans,trans-farnesyl-L-cysteine (AFC) have previously been shown to inhibit the carboxyl methylation of proteins carrying a C-terminal S-prenylated cysteine residue and to block the endotoxin-activated serum-elicited chemotactic response of mouse macrophages. Here, we...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Scheer A,Gierschik P

    更新日期:1993-03-15 00:00:00

  • Effect of the phorbol ester TPA on PTH secretion. Evidence for a role for protein kinase C in the control of PTH release.

    abstract::Parathyroid hormone (PTH) secretion is stimulated by low extracellular calcium (Ca2+) in association with a reduction in cyosolic Ca2+, indicating that this cell type does not conform to classical models of stimulus-secretion coupling. We used the phorbol ester TPA (12-O-tetradecanoyl phorbol 13-acetate), which direct...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Brown EM,Redgrave J,Thatcher J

    更新日期:1984-09-17 00:00:00

  • Low-density lipoproteins interact with liposome-binding sites on the cell surface.

    abstract::Under physiological conditions significant amounts of low-density lipoprotein LDL particles ar taken up by cells independently of specific high-affinity LDL receptors (apo-B receptors). Previously it was established that some cells contain surface sites capable of binding liposomes. We proposed that liposome-binding s...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Galkina SI,Ivanov VV,Preobrazhensky SN,Margolis LB,Bergelson LD

    更新日期:1991-08-05 00:00:00

  • A reverse genetics system of African horse sickness virus reveals existence of primary replication.

    abstract::African horse sickness virus (AHSV), a member of the orbivirus genus of the family Reoviridae, is an insect-vectored pathogen of horses of concern to the equine industry. Studies on AHSV replication and pathogenesis have been hampered by the lack of reverse genetics allowing targeted mutation of viral genomes. We demo...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Matsuo E,Celma CC,Roy P

    更新日期:2010-08-04 00:00:00

  • The mystery of the trichothecene 3-O-acetyltransferase gene. Analysis of the region around Tri101 and characterization of its homologue from Fusarium sporotrichioides.

    abstract::The trichothecene 3-O-acetyltransferase gene, Tri101, plays a pivotal role for the well-being of the type B trichothecene producer Fusarium graminearum. We have analyzed the cosmids containing Tri101 and found that this resistance gene is not in the biosynthetic gene cluster reported so far. It was located between the...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kimura M,Matsumoto G,Shingu Y,Yoneyama K,Yamaguchi I

    更新日期:1998-09-18 00:00:00

  • Conversion of the sodium channel activator aconitine into a potent alpha 7-selective nicotinic ligand.

    abstract::Methyllycaconitine (MLA) is a competitive antagonist of nicotinic acetylcholine receptors, with a remarkable preference for neuronal [125I]alpha Bgt binding sites. We have begun to investigate the structural basis of its potency and subtype selectivity. MLA is a substituted norditerpenoid alkaloid linked to a 2-(methy...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hardick DJ,Cooper G,Scott-Ward T,Blagbrough IS,Potter BV,Wonnacott S

    更新日期:1995-05-22 00:00:00