Abstract:
:Tyrosine-rich acidic matrix protein (TRAMP; 22 kDa extracellular matrix protein; dermatopontin) is a protein that co-purifies with lysyl oxidase and with dermatan sulphate proteoglycans, with possible functions in cell-matrix interactions and matrix assembly. Using a rabbit polyclonal antiserum raised against porcine TRAMP, which cross-reacts with both the human and murine forms of the protein, we show by immunoblotting that TRAMP has a widespread tissue distribution, including skin, skeletal muscle, heart, lung, kidney, cartilage and bone. In cultures of human skin fibroblasts, TRAMP incorporates both [35S]sulphate and [3H]tyrosine and is secreted into the medium, as shown by immunoprecipitation. Amino acid analysis of immunoprecipitated TRAMP demonstrates that many of the tyrosine residues in TRAMP are sulphated.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Forbes EG,Cronshaw AD,MacBeath JR,Hulmes DJdoi
10.1016/0014-5793(94)00907-4subject
Has Abstractpub_date
1994-09-12 00:00:00pages
433-6issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(94)00907-4journal_volume
351pub_type
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