Empirical free energy as a target function in docking and design: application to HIV-1 protease inhibitors.

abstract：:S100, a subfamily of the EF-hand type calcium sensing proteins, is implicated in many cellular functions including muscle contractility. Two isoforms, S100A1 and S100B, at 2-10 microM significantly inhibit active tension, stiffness and ATPase of skinned single rabbit psoas muscle fibers at sub-maximal (pCa approximate...

journal_title：FEBS letters

authors： Adhikari BB,Wang K

更新日期：2001-05-25 00:00:00

abstract：:The phosphopeptide Ser (P)-Ser(P)-Ser-(P)-Glu-Glu-Ser22-Ile-Thr, reproducing the 17-24 segment of beta-casein A2 including the seryl residue (Ser-22) which is targeted by casein kinase-1 was synthesized and used as model substrate for this enzyme. Its phosphorylation efficiency is actually higher than that of intact b...

journal_title：FEBS letters

authors： Meggio F,Perich JW,Reynolds EC,Pinna LA

更新日期：1991-06-03 00:00:00

abstract：:Various cDNAs coding for part or all of human pre-pro lactase-phlorizin hydrolase (pre-proLPH) were transfected into COS cells and the subcellular location of the lactase-related proteins assessed. Only the complete proLPH reached the plasma membrane. LPH without the pro sequence, and a construct containing the pro se...

journal_title：FEBS letters

authors： Oberholzer T,Mantei N,Semenza G

更新日期：1993-10-25 00:00:00

abstract：:Lipid droplets (LDs) were perceived as static storage deposits, which passively participate in the energy homeostasis of both cells and entire organisms. However, this view has changed recently after the realization of a complex and highly dynamic LD proteome. The proteome contains key components of the fat mobilizati...

journal_title：FEBS letters

authors： Beller M,Thiel K,Thul PJ,Jäckle H

更新日期：2010-06-03 00:00:00

abstract：:SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)1H-imi dazole) is widely used as a specific inhibitor of p38 mitogen-activated protein kinase (MAPK). Here, we report that SB203580 activates the serine/threonine kinase Raf-1 in quiescent smooth muscle cells in a dose-dependent fashion. The concentr...

journal_title：FEBS letters

authors： Kalmes A,Deou J,Clowes AW,Daum G

更新日期：1999-02-05 00:00:00

abstract：:The mutation of Asp198 to Asn in the receptor for glucagon-like peptide-1(7-36)amide (GLP-1) had no effect upon GLP-1 affinity whereas substitution with Ala greatly reduced affinity, demonstrating the importance of polarity rather than negative charge at Asp198. However, the Asp198-Ala mutation had less effect upon th...

journal_title：FEBS letters

authors： López de Maturana R,Donnelly D

更新日期：2002-10-23 00:00:00

abstract：:Incubation of cholinergic synaptic vesicles purified from the electric organ of Torpedo ocellata with radiolabeled [D-Ala2, D-Leu5]-enkephalin results in its accumulation in the vesicles. Kinetic analysis of the initial rates of uptake reveals temperature-dependent saturation kinetics which are best fitted by a single...

journal_title：FEBS letters

authors： Day NC,Wien D,Michaelson DM

更新日期：1985-04-08 00:00:00

abstract：:Chlorella virus-encoded membrane protein Kcv represents a new class of potassium channel. This 94-amino acids miniature K(+) channel consists of two trans-membrane alpha-helix domains intermediated by a pore domain that contains a highly conserved K(+) selectivity filter. Therefore, as an archetypal K(+) channel, the ...

journal_title：FEBS letters

authors： Shim JW,Yang M,Gu LQ

更新日期：2007-03-06 00:00:00

abstract：:Coenzyme A ligases play an important role in metabolism by catalyzing the activation of carboxylic acids. In this study we describe the synthesis of aminoacyl-coenzyme As (CoAs) catalyzed by a CoA ligase from Penicillium chrysogenum. The enzyme accepted medium-chain length fatty acids as the best substrates, but the p...

journal_title：FEBS letters

authors： Koetsier MJ,Jekel PA,Wijma HJ,Bovenberg RA,Janssen DB

更新日期：2011-03-23 00:00:00

abstract：:The anomolous protein synthesis, sensitive to cycloheximide, which has been observed in brain mitochondrial and synaptosomal preparations, has been studied. It is concluded that this protein synthesis is due to the presence, as a contaminant in both preparations, of a ribosome-containing particle which contains solubl...

journal_title：FEBS letters

authors： Morgan IG

更新日期：1970-10-16 00:00:00

abstract：:2-( Dimethylaminostyryl )-l-methyl pyridinium, a fluorescent indicator of mitochondrial membrane potential, is accumulated up to 400-fold by intact, isolated brown adipocytes from cold-adapted guinea pigs. The indicator can be optically localized within the in situ mitochondria. Ionophores which would be predicted to ...

journal_title：FEBS letters

authors： Rafael J,Nicholls DG

更新日期：1984-05-07 00:00:00

abstract：:In Saccharomyces cerevisiae, four proteins, Shu1, Shu2, Psy3 and Csm2, form a stable SHU-complex both in vivo and in vitro. These proteins are involved in the early stages of the homologous recombination DNA damage repair process. In this paper, the crystal structure of the Psy3-Csm2 sub-complex is presented at 1.8Å r...

journal_title：FEBS letters

authors： She Z,Gao ZQ,Liu Y,Wang WJ,Liu GF,Shtykova EV,Xu JH,Dong YH

更新日期：2012-07-30 00:00:00

abstract：:Even though the plant photoreceptors cryptochromes were discovered more than 20 years ago, the mechanism through which they transduce light signals to their partner molecules such as COP1 (Constitutive Photomorphogenic 1) or SPA1 (Suppressor of Phytochrome A) still remains to be established. We propose that a negative...

journal_title：FEBS letters

authors： Müller P,Bouly JP

更新日期：2015-01-16 00:00:00

abstract：:In Drosophila, the tumor suppressor Scribble is localized at the septate junctions of epithelial cells. Its mammalian homologue, hScrib, is a basolateral protein likely associated to proteins of the cell-cell junctions. We report the direct interaction between hScrib and ZO-2, a junction-associated protein. This inter...

journal_title：FEBS letters

authors： Métais JY,Navarro C,Santoni MJ,Audebert S,Borg JP

更新日期：2005-07-04 00:00:00

abstract：:To address biophysical principles and lipid interactions that underlie the properties of membrane proteins, modifications that vary the neighbors of tryptophan residues in the highly dynamic transmembrane helix of GW4,20 ALP23 (acetyl-GGAW4 A(LA)6 LAW20 AGA-amide) were examined using deuterium NMR spectroscopy. It was...

journal_title：FEBS letters

authors： McKay MJ,Greathouse DV,Koeppe RE 2nd

更新日期：2020-12-01 00:00:00

abstract：:The marked difference in auxin sensitivity between plant roots and shoots was studied in terms of auxin-induced stimulation of membrane P-type ATPase. The results suggest the existence in rice seedlings of, at least, two isoforms of soluble proteinous factors (SPF), SPF(I) and SPF(II), which are involved in the stimul...

journal_title：FEBS letters

authors： Kim D,Kim YS,Jung J

更新日期：1997-06-09 00:00:00

abstract：:We show that N-1 in adenine of chromosomal DNA is methylated by treatment of metaphase chromosomes with dimethylsulphate while this is not the case in chromatin. The data on methylation are consistent with those obtained from the experiments with S1-nuclease treatment of chromatin and chromosomes. This suggests a disa...

journal_title：FEBS letters

authors： Belyaev ND,Budker VG,Dubrovskaya VA,Matveeva NM

更新日期：1983-04-18 00:00:00

abstract：:Acrosin inhibitors of seminal vesicle origin, after binding to their acceptor molecules on the anterior part of ejaculated sperm, are thought to be important capacitation factors, protecting zona binding sites during sperm uterine passage, and then dissociating to allow sperm binding to the zona pellucida of the oocyt...

journal_title：FEBS letters

authors： Jonáková V,Calvete JJ,Mann K,Schäfer W,Schmid ER,Töpfer-Petersen E

更新日期：1992-02-03 00:00:00

abstract：:Syntabulin is a microtubule-associated protein that mediates anterograde transport of vesicles to neuronal processes. Here, we found that syntabulin was expressed in mouse pancreas and insulin-secreting β-cells, and that it partially co-localized with microtubule and insulin-containing granules. The association of syn...

journal_title：FEBS letters

authors： Ying Y,Li L,Cao W,Yan D,Zeng Q,Kong X,Lu L,Yan M,Xu X,Qu J,Su Q,Ma X

更新日期：2012-10-19 00:00:00

abstract：:Extraction and identification of the non-covalently bound heme groups from crude membrane preparations of photoheterotrophically grown Synechocystis sp. PCC 6803 by reversed phase high performance liquid chromatography and optical spectrophotometry led to the detection of heme O in addition to hemes B and A which latt...

journal_title：FEBS letters

authors： Peschek GA,Wastyn M,Fromwald S,Mayer B

更新日期：1995-09-04 00:00:00

abstract：:Using top-of-the-line fold recognition methods, we assigned an oligonucleotide/oligosaccharide-binding (OB)-fold structure to a family of previously uncharacterized hypothetical proteins from several bacterial genomes. This novel family of bacterial OB-fold (BOF) proteins present in a number of pathogenic strains enco...

journal_title：FEBS letters

authors： Ginalski K,Kinch L,Rychlewski L,Grishin NV

更新日期：2004-06-04 00:00:00

abstract：:Holocytochrome c synthase (HCCS) attaches heme covalently to mitochondrial respiratory cytochromes c. Little is known about the reaction of heme attachment to apocytochromes c by HCCS, although recently it has been established that the CXXCH motif and the N-terminus of the apocytochrome polypeptide are important prote...

journal_title：FEBS letters

authors： Zhang Y,Stevens JM,Ferguson SJ

更新日期：2014-09-17 00:00:00

abstract：:We have successfully delivered FITC and FITC-Dextran (70, 250 kDa) into canola protoplasts by centrifuging cells with different amounts of microbubbles at variable centrifuge speed. The efficiency is around 90%, while cell viability remains high. Confocal microscopy images show that both FITC and FITC-Dextran are scat...

journal_title：FEBS letters

authors： He C,Gu Q,Huang M,Xing J,Chen J

更新日期：2013-01-31 00:00:00

abstract：:SARS 8b is one of the putative accessory proteins of the severe acute respiratory syndrome-associated coronavirus (SARS-CoV) with unknown functions. In this study, the cellular localization and activity of this estimated 9.6 kDa protein were examined. Confocal microscopy results indicated that SARS 8b is localized in ...

journal_title：FEBS letters

authors： Law PY,Liu YM,Geng H,Kwan KH,Waye MM,Ho YY

更新日期：2006-06-26 00:00:00

abstract：:14-3-3 proteins were the first signaling molecules to be identified as discrete phosphoserine/threonine binding modules. This family of proteins, which includes seven isotypes in human cells and up to 15 in plants, plays critical roles in cell signaling events that control progress through the cell cycle, transcriptio...

journal_title：FEBS letters

authors： Yaffe MB

更新日期：2002-02-20 00:00:00

abstract：:Two phosphoenolpyruvate carboxylase (PEPC) kinase genes (PPCk1 and PPCk2) are present in the Arabidopsis genome; only PPCk1 is expressed in rosette leaves. Homozygous lines of two independent PPCk1 T-DNA-insertional mutants showed very little (dln1), or no (csi8) light-induced PEPC phosphorylation and a clear retard i...

journal_title：FEBS letters

authors： Meimoun P,Gousset-Dupont A,Lebouteiller B,Ambard-Bretteville F,Besin E,Lelarge C,Mauve C,Hodges M,Vidal J

更新日期：2009-05-19 00:00:00

abstract：:Human prothymosin alpha mutants were generated with the aid of random mutagenesis and screened for their ability to inhibit yeast Saccharomyces cerevisiae cell growth. Conversion of Lys-87 to Glu resulted in an inactivated prothymosin alpha mutant, which lost the ability of the wild-type protein to block yeast cell gr...

journal_title：FEBS letters

authors： Rubtsov Y,Vartapetian A

更新日期：1996-11-18 00:00:00

abstract：:The molecular chaperone protein Hsp78, a member of the Clp/Hsp100 family localized in the mitochondria of Saccharomyces cerevisiae, is required for maintenance of mitochondrial functions under heat stress. To characterize the biochemical mechanisms of Hsp78 function, Hsp78 was purified to homogeneity and its role in t...

journal_title：FEBS letters

authors： Krzewska J,Langer T,Liberek K

更新日期：2001-01-26 00:00:00

abstract：:The mutation of a single leucine residue (L38) to methionine (M) is known experimentally to significantly increase the affinity of the synthetic four-alpha-helix bundle (Aalpha(2))(2) for the anesthetic halothane. We present a molecular dynamics study of the mutant (Aalpha(2)-L38M)(2) peptide, which consists of a dime...

journal_title：FEBS letters

authors： Davies LA,Zhong Q,Klein ML,Scharf D

更新日期：2000-07-28 00:00:00

abstract：:The domains of the pore-forming protein responsible for the gate and channel formations were separated and identified in the outer membrane of Pseudomonas aeruginosa. The proteolytic cleavage of the 46K channel protein, protein D2, yielded two major domains with apparent M(r) of 27K and 19K. We identified the 27K poly...

journal_title：FEBS letters

authors： Yoshihara E,Nakae T

更新日期：1992-07-13 00:00:00