Abstract:
:Structural gene expression of human immunodeficiency virus type 1 (HIV-1) requires a viral regulatory protein, Rev transactivator. We investigated Rev-dependency of HIV-1 gene expression by various reporter systems. Expression of unspliced and single-spliced viral mRNAs was demonstrated to be differentially dependent on the Rev function. This difference of Rev-dependency was found not to be determined by cis-elements in gag, pol, and env coding sequences reported so far, and was lost when the reporter constructs containing minimum elements for Rev-responsiveness such as splice signals and rev responsive element were used for experiments. These findings indicated that the fundamental structure of HIV-1 mRNA was critical for the differential regulation of gene expression by Rev transactivator.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Sakai H,Furuta RA,Tokunaga K,Kawamura M,Hatanaka M,Adachi Adoi
10.1016/0014-5793(95)00444-esubject
Has Abstractpub_date
1995-05-29 00:00:00pages
141-5issue
2-3eissn
0014-5793issn
1873-3468pii
0014-5793(95)00444-Ejournal_volume
365pub_type
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