Abstract:
:Rat glutathione transferase 8-8 is one of the less abundant cytosolic glutathione transferases, accounting for approx. 1% of the total activity with 1-chloro-2,4-dinitrobenzene in liver. The enzyme is eluted at pH 6.3 upon chromatofocusing and has so far been identified in liver, kidney, lung and testis. Characteristic properties include high relative activity with ethacrynic acid (70% of the specific activity with 1-chloro-2,4-dinitrobenzene) and an apparent subunit Mr of 24 500. The most significant property noted is the high catalytic activity in the conjugation of 4-hydroxyalk-2-enals, major products of lipid peroxidation. The catalytic efficiency with these substrates exceeds corresponding values for all known substrates tested with any glutathione transferase, which suggests that transferase 8-8 may have evolved to detoxify 4-hydroxyalk-2-enals.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Jensson H,Guthenberg C,Alin P,Mannervik Bdoi
10.1016/0014-5793(86)80743-8subject
Has Abstractpub_date
1986-07-28 00:00:00pages
207-9issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(86)80743-8journal_volume
203pub_type
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