Diffusion of fluorescein-labelled molecules in suspensions of erythrocyte ghosts.

abstract：:The FMN-heme interdomain (intraprotein) electron transfer (IET) kinetics in full length and oxygenase/FMN (oxyFMN) construct of human iNOS were determined by laser flash photolysis over the temperature range from 283 to 304K. An appreciable increase in the rate constant value was observed with an increase in the tempe...

journal_title：FEBS letters

authors： Li W,Chen L,Fan W,Feng C

更新日期：2012-01-20 00:00:00

abstract：:Plasma citrate levels were found to be elevated in non-alcoholic fatty liver disease (NAFLD) patients. Cellular experiments indicated that increased citrate levels might originate from an excess of fatty acids. The impact of elevated citrate levels on oxidative stress was examined. It was found that citrate stimulated...

journal_title：FEBS letters

authors： van de Wier B,Balk JM,Haenen GR,Giamouridis D,Bakker JA,Bast BC,den Hartog GJ,Koek GH,Bast A

更新日期：2013-08-02 00:00:00

abstract：:Yeast Golgi ecto-ATPase Ynd1p is an unusual type III membrane protein with the longest translocated N-terminus reported. Sequential deletion analysis reveals that translocation of this 500-residue-long hydrophilic domain across the membranes requires the C-terminal transmembrane domain of Ynd1p and its flanking region...

journal_title：FEBS letters

authors： Zhong X,Malhotra R,Guidotti G

更新日期：2005-10-24 00:00:00

abstract：:Dimethylsulphoxide (DMSO) reductase from R. capsulatus contains a molybdenum-pterin cofactor at its active site. As prepared the molybdenum is in the 6+ oxidation state, devoid of EPR signals. Stepwise reduction generates an EPR signal characteristic of Mo(V) having hyperfine coupling to a single proton and integratin...

journal_title：FEBS letters

authors： Benson N,Farrar JA,McEwan AG,Thomson AJ

更新日期：1992-07-28 00:00:00

abstract：:A recombinant antigen (p22-NS3), possessing putative HCV nucleocapsid protein (p22) and non-structural protein 3 (NS3) epitopes, was heavily expressed in E. coli and purified. The p22-NS3 purified recombinant antigen strongly reacts with sera containing human antibodies directed against p22 and NS3 providing a startin...

journal_title：FEBS letters

authors： Osborne S,Cecconato E,Griva S,Garetto F,Calogero R,Rosa C,Bonelli F

更新日期：1993-06-21 00:00:00

abstract：:The Kv3.1 channel plays a crucial role in regulating the high-frequency firing properties of neurons. Here, we determined whether Src regulates the subcellular distributions of the Kv3.1b channel. Co-expression of active Src induced a dramatic redistribution of Kv3.1b to the endoplasmic reticulum. Furthermore, co-expr...

journal_title：FEBS letters

authors： Bae SH,Kim DH,Shin SK,Choi JS,Park KS

更新日期：2014-01-03 00:00:00

abstract：:The activity of calcium, phospholipid-dependent protein kinase (PKc), which is thought to play an important role in cell proliferation, has been measured in the particulate and soluble fractions of cultured cells, under different proliferative conditions. Our results indicate that proliferating cells display higher PK...

journal_title：FEBS letters

authors： Adamo S,Caporale C,Aguanno S,Lazdins J,Faggioni A,Belli L,Cortesi E,Nervi C,Gastaldi R,Molinaro M

更新日期：1986-01-20 00:00:00

abstract：:A UV irradiation-induced mutant of Bacillus sphaericus 2362 whose sporulation was inhibited neither by natural amino acids nor by L-ethionine was selected. The mutant (A61) grew slowly in rich amino acid medium and contained increased concentrations of heat-resistant spores throughout the growth. Slow growth of A61 wa...

journal_title：FEBS letters

authors： Andreev J,Dibrov PA,Braun S

更新日期：1994-06-27 00:00:00

abstract：:A serum-free medium conditioned by U937, a human cell line of monocyte/macrophage origin, was found capable of inducing apoptosis on exponentially growing U937 cells themselves (autocrine suicide). The apoptosis-inducing agent is a macromolecule and possibly a protein (SKT factor), with a relative molecular mass in th...

journal_title：FEBS letters

authors： Ghibelli L,Coppola S,Nosseri C,Bergamini A,Beninati S

更新日期：1994-05-09 00:00:00

abstract：:We report isolation of a murine gene, NPYR-D, which predicts an intronless novel G protein-coupled receptor of 375 amino acids. Percent identities of NPYR-D to the clone Y1, Y2, rat Y4/PP1 and human Y4/PP1 receptors are 45, 32, 92 and 76, respectively. Southern blots indicate that NPYR-D and human Y4/PP1 receptor gene...

journal_title：FEBS letters

authors： Gregor P,Millham ML,Feng Y,DeCarr LB,McCaleb ML,Cornfield LJ

更新日期：1996-02-26 00:00:00

abstract：:Transient-receptor-potential channels (TRPs) underlie the sensing of chemicals, heat, and mechanical force. We expressed the rat TRPV1 and TRPV4 subtypes in yeast and monitored their activities in vivo as Ca(2+) rise using transgenic aequorin. Heat and capsaicin activate TRPV1 but not TRPV4 in yeast. Hypotonic shocks ...

journal_title：FEBS letters

authors： Loukin SH,Su Z,Kung C

更新日期：2009-02-18 00:00:00

abstract：:The structure of [Val1]gramicidin A incorporated into sodium dodecyl-d25 sulphate micelles has been studied by two-dimensional proton NMR spectroscopy. Analysis of nuclear Overhauser effects, spin-spin couplings and solvent accessibility of NH groups show that the conformation of the Na+ complex of gramicidin A in det...

journal_title：FEBS letters

authors： Arseniev AS,Barsukov IL,Bystrov VF,Lomize AL,Ovchinnikov YuA

更新日期：1985-07-08 00:00:00

abstract：:Acute hyperammonemia, induced by two consecutive injections of ammonium acetate (550 and 450 mg per kg b.wt.), decreased the activity of gamma-glutamyl transpeptidase (GGT) in most brain regions of 18- and 30-day-old rats. This decrease in the brain GGT activity was more pronounced in younger than in older rats. After...

journal_title：FEBS letters

authors： Stastný F,Pitha J,Lisý V,Hilgier W,Kaucká I,Albrecht J

更新日期：1992-04-06 00:00:00

abstract：:Hepatitis B virus X protein is a major factor in the HBV-induced disease developments. Stromal cell-derived factor-1 is a small cytokine that is strongly chemotactic for lymphocytes. We explored the role of HBx on recruitment of HBV-induced virus-nonspecific immune cells into liver. Immune cell recruitment and SDF-1 e...

journal_title：FEBS letters

authors： Cho HK,Kim SY,Seong JK,Cheong J

更新日期：2014-03-03 00:00:00

abstract：:This study demonstrated that activation of tyrosine kinase of epidermal growth factor receptor (EGFR) induces its association with G protein-coupled receptor kinase 2 (GRK2). Immunoprecipitation experiments showed that EGF stimulation increased GRK2 binding to EGFR complex in HEK293 cells coexpressing EGFR and GRK2. T...

journal_title：FEBS letters

authors： Gao J,Li J,Chen Y,Ma L

更新日期：2005-01-03 00:00:00

abstract：:Tomato Cf-9, a receptor-like protein (RLP), confers resistance to races of the fungal pathogen Cladosporium fulvum that express the Avr9 avirulence gene. CITRX (Cf-9-interacting thioredoxin) was previously identified in a yeast two-hybrid screen as a protein interacting with the cytoplasmic domain of Cf-9 and shown to...

journal_title：FEBS letters

authors： Nekrasov V,Ludwig AA,Jones JD

更新日期：2006-07-24 00:00:00

abstract：:Respiratory complexes in both prokaryotes and eukaryotes contain multiple co-factors, which are coordinated in defined positions so that they can function as electron wires. Intriguingly, co-factors are usually buried deep within hetero-oligomeric protein complexes and it is not clear when or how they are incorporated...

journal_title：FEBS letters

authors： Palombo I,Daley DO

更新日期：2012-11-30 00:00:00

abstract：:Higher basal 2-deoxy-D-glucose uptake in rheumatoid synovial cells than in non-rheumatoid synovial cells, was found to be associated with an increased interleukin-1 beta (IL-1 beta) secretion (respectively 850 +/- 238 vs. 8.3 +/- 2.4 pg/24 h/10(5) cells, mean +/- S.E.M.). When exogenous human recombinant IL-1 beta was...

journal_title：FEBS letters

authors： Hernvann A,Aussel C,Cynober L,Moatti N,Ekindjian OG

更新日期：1992-05-25 00:00:00

abstract：:Redox reactions of tyrosine play key roles in many biological processes, including water oxidation and DNA synthesis. We first review the redox properties of tyrosine (and other phenols) in small molecules and related polypeptides, then report work on (H20)/(Y48)-modified Pseudomonas aeruginosa azurin. The crystal str...

journal_title：FEBS letters

authors： Warren JJ,Winkler JR,Gray HB

更新日期：2012-03-09 00:00:00

abstract：:As a member of the dynamin superfamily human guanylate-binding protein 1 (hGBP1) binds and hydrolyses GTP thereby undergoing structural changes which lead to self-assembly of the protein. Here, we employ the reactivity of hGBP1 with a cysteine reactive compound in order to monitor structural changes imposed by GTP bin...

journal_title：FEBS letters

authors： Vöpel T,Kunzelmann S,Herrmann C

更新日期：2009-06-18 00:00:00

abstract：:In this study we identify the molecules involved in the MAPK signal transduction pathway (Ras, Raf-1, Mek, Mek-P and MAPK) in highly purified endosomal fractions isolated from rat liver. Biochemical analysis shows that only the early-sorting endocytic compartment contains activated Raf-1 and Mek. Finally, the exogenou...

journal_title：FEBS letters

authors： Pol A,Calvo M,Enrich C

更新日期：1998-12-11 00:00:00

abstract：:Laser flash-induced photovoltage responses of the D85S and D85T mutants as well as of the wild-type acid blue form are similar and reflect intraprotein charge redistribution caused by retinal isomerization. The Cl- -induced transition of all of these blue forms into purple ones is accompanied by the appearance of elec...

journal_title：FEBS letters

authors： Kalaidzidis IV,Kaulen AD

更新日期：1997-12-01 00:00:00

abstract：:The glycogen-binding (G) subunit of protein phosphatase-1 is phosphorylated in vivo. In rabbits injected with propranolol the serine residue termed site-1 was phosphorylated in 56% of the molecules isolated, and phosphorylation increased to 82% after administration of adrenalin. It is concluded that the G-subunit is a...

journal_title：FEBS letters

authors： MacKintosh C,Campbell DG,Hiraga A,Cohen P

更新日期：1988-07-04 00:00:00

abstract：:It was found for RNases of different specificities that changes in the free energy for substrate-enzyme binding induced by variations in the nucleotide base structure are accompanied by proportional changes in kcat/Km. This was considered to be a consequence of the strain in the enzyme-substrate complex. ...

journal_title：FEBS letters

authors： Yakovlev GI,Bocharov AL,Moiseyev GP

更新日期：1984-10-01 00:00:00

abstract：:With the ultimate aim of developing an effective antiviral strategy against HIV-1, a mono-DNA enzyme possessing the 10-23 catalytic motif [Santoro and Joyce (1997) Proc. Natl. Acad. Sci. USA 94, 4264-4266] was synthesized against the HIV-1 envelope gene. We tested the in vitro cleavage efficiency of the 178 bp long tr...

journal_title：FEBS letters

authors： Dash BC,Harikrishnan TA,Goila R,Shahi S,Unwalla H,Husain S,Banerjea AC

更新日期：1998-07-24 00:00:00

abstract：:A role for the cytokines produced by tissue-infiltrated inflammatory cells (mainly T-lymphocytes and mast cells) in the pathophysiology of fibrosis has been suggested by several groups. Among the products of these cells, interleukin-4 (IL-4) might be one of the factors involved in the initiation of the fibrotic proces...

journal_title：FEBS letters

authors： Gillery P,Fertin C,Nicolas JF,Chastang F,Kalis B,Banchereau J,Maquart FX

更新日期：1992-05-18 00:00:00

abstract：:F-box proteins play pivotal roles in multiple cellular processes; however, little is known about their functions in glioma progression. In this study, we found that expression of the F-box and leucine-rich repeat protein 18 (FBXL18) is significantly upregulated in glioma tissues. Depletion of FBXL18 in glioma cells su...

journal_title：FEBS letters

authors： Zhang J,Yang Z,Ou J,Xia X,Zhi F,Cui J

更新日期：2017-01-01 00:00:00

abstract：:A cDNA encoding stromal ascorbate peroxidase (sAPX) was isolated using poly(A)+ RNA of pumpkin cotyledons by RT-PCR. The cDNA encodes a polypeptide with 372 amino acids and shares complete sequence identity with pumpkin thylakoid-bound ascorbate peroxidase (tAPX), except for the deletion of a putative membrane spannin...

journal_title：FEBS letters

authors： Mano S,Yamaguchi K,Hayashi M,Nishimura M

更新日期：1997-08-11 00:00:00

abstract：:We have identified in rat vascular smooth muscle cells (SMCs) the simultaneous expression of two TGF-beta type I receptor (ALK-5) cDNAs, occurring as a consequence of alternate usage of AG splice acceptor motifs separated by 12 nucleotides located at an intron-exon junction. When translated the resultant full length p...

journal_title：FEBS letters

authors： Agrotis A,Condron M,Bobik A

更新日期：2000-02-04 00:00:00

abstract：:Formylmethanofuran dehydrogenase, a key enzyme of methanogenesis, was purified 100-fold from methanol grown Methanosarcina barkeri to apparent homogeneity and a specific activity of 34 mumol.min-1.mg protein-1. Molybdenum was found to co-migrate with the enzyme activity. The molybdenum content of purified preparations...

journal_title：FEBS letters

authors： Karrasch M,Börner G,Enssle M,Thauer RK

更新日期：1989-08-14 00:00:00