Abstract:
:Transient-receptor-potential channels (TRPs) underlie the sensing of chemicals, heat, and mechanical force. We expressed the rat TRPV1 and TRPV4 subtypes in yeast and monitored their activities in vivo as Ca(2+) rise using transgenic aequorin. Heat and capsaicin activate TRPV1 but not TRPV4 in yeast. Hypotonic shocks activate TRPV4 but not TRPV1. Osmotic swelling is modeled to activate enzyme(s), producing polyunsaturated fatty acids (PUFAs) to open TRPV4 in mammalian cells. This model relegates mechanosensitivity to the enzyme and not the channel. Yeast has only a single Delta9 fatty-acid monodesaturase and cannot make PUFAs suggesting an alternative mechanism for TRPV4 activation. We discuss possible explanations of this difference.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Loukin SH,Su Z,Kung Cdoi
10.1016/j.febslet.2009.01.027subject
Has Abstractpub_date
2009-02-18 00:00:00pages
754-8issue
4eissn
0014-5793issn
1873-3468pii
S0014-5793(09)00049-0journal_volume
583pub_type
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