Hypotonic shocks activate rat TRPV4 in yeast in the absence of polyunsaturated fatty acids.

Abstract:

:Transient-receptor-potential channels (TRPs) underlie the sensing of chemicals, heat, and mechanical force. We expressed the rat TRPV1 and TRPV4 subtypes in yeast and monitored their activities in vivo as Ca(2+) rise using transgenic aequorin. Heat and capsaicin activate TRPV1 but not TRPV4 in yeast. Hypotonic shocks activate TRPV4 but not TRPV1. Osmotic swelling is modeled to activate enzyme(s), producing polyunsaturated fatty acids (PUFAs) to open TRPV4 in mammalian cells. This model relegates mechanosensitivity to the enzyme and not the channel. Yeast has only a single Delta9 fatty-acid monodesaturase and cannot make PUFAs suggesting an alternative mechanism for TRPV4 activation. We discuss possible explanations of this difference.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Loukin SH,Su Z,Kung C

doi

10.1016/j.febslet.2009.01.027

subject

Has Abstract

pub_date

2009-02-18 00:00:00

pages

754-8

issue

4

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(09)00049-0

journal_volume

583

pub_type

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