Abstract:
:To test if mitochondrial uncoupling in white adipocytes is responsible for obesity resistance of the aP2-Ucp transgenic mice expressing ectopic uncoupling protein 1 (UCPI) in white fat, mitochondrial membrane potential (delta psi(m)) was estimated by flow cytometry in adipocytes isolated from gonadal fat. Ectopic UCP1 (approximately 0.8 mol UCP1/mol respiratory chain) decreased the delta psi(m) and rendered the potential sensitive to GDP and fatty acids. These ligands of UCP1 had no effect on delta psi(m) in white adipocytes from non-transgenic mice, suggesting that the function of endogenous UCP2 in adipocytes was not affected. The results support the hypothesis that mitochondrial uncoupling in white fat may prevent development of obesity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Baumruk F,Flachs P,Horáková M,Floryk D,Kopecký Jdoi
10.1016/s0014-5793(99)00053-8keywords:
subject
Has Abstractpub_date
1999-02-12 00:00:00pages
206-10issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(99)00053-8journal_volume
444pub_type
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