Transgenic UCP1 in white adipocytes modulates mitochondrial membrane potential.

Abstract:

:To test if mitochondrial uncoupling in white adipocytes is responsible for obesity resistance of the aP2-Ucp transgenic mice expressing ectopic uncoupling protein 1 (UCPI) in white fat, mitochondrial membrane potential (delta psi(m)) was estimated by flow cytometry in adipocytes isolated from gonadal fat. Ectopic UCP1 (approximately 0.8 mol UCP1/mol respiratory chain) decreased the delta psi(m) and rendered the potential sensitive to GDP and fatty acids. These ligands of UCP1 had no effect on delta psi(m) in white adipocytes from non-transgenic mice, suggesting that the function of endogenous UCP2 in adipocytes was not affected. The results support the hypothesis that mitochondrial uncoupling in white fat may prevent development of obesity.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Baumruk F,Flachs P,Horáková M,Floryk D,Kopecký J

doi

10.1016/s0014-5793(99)00053-8

keywords:

subject

Has Abstract

pub_date

1999-02-12 00:00:00

pages

206-10

issue

2-3

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(99)00053-8

journal_volume

444

pub_type

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