Abstract:
:Snake venoms are rich sources of serine proteinase inhibitors that are members of the Kunitz/BPTI (bovine pancreatic trypsin inhibitor) family. However, only a few of their gene sequences have been determined from snakes. We therefore cloned the cDNAs for the trypsin and chymotrypsin inhibitors from a Vipera ammodytes venom gland cDNA library. Phylogenetic analysis of these and other snake Kunitz/BPTI homologs shows the presence of three clusters, where sequences cluster by functional role. Analysis of the nucleotide sequences from the snake Kunitz/BPTI family shows that positive Darwinian selection was operating on the highly conserved BPTI fold, indicating that this family evolved by gene duplication and rapid diversification.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Zupunski V,Kordis D,Gubensek Fdoi
10.1016/s0014-5793(03)00693-8keywords:
subject
Has Abstractpub_date
2003-07-17 00:00:00pages
131-6issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579303006938journal_volume
547pub_type
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