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An improved purification of lactose permease.

Abstract：

:The integral membrane protein lactose permease of Escherichia coli was purified to homogeneity in detergent micelles. No other proteins could be detected in the final product. The molar ratio of lactose permease to lipid was less than 0.2 in detergent solution, thus the preparation was essentially lipid-free. All molecules were functionally active as shown by substrate binding.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Dornmair K

doi

10.1016/0014-5793(88)81229-8

subject

Has Abstract

pub_date

1988-08-01 00:00:00

pages

35-9

issue

1-2

eissn

0014-5793

issn

1873-3468

pii

0014-5793(88)81229-8

journal_volume

235

pub_type

杂志文章

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