FHA domain boundaries of the dun1p and rad53p cell cycle checkpoint kinases.

Abstract:

:Dun1p and Rad53p of the budding yeast Saccharomyces cerevisiae are members of a conserved family of cell cycle checkpoint protein kinases that contain forkhead-associated (FHA) domains. Here, we demonstrate that these FHA domains contain 130-140 residues, and are thus considerably larger than previously predicted by sequence comparisons (55-75 residues). In vivo, expression of the proteolytically defined Dun1p FHA domain, but not a fragment containing only the predicted domain boundaries, inhibited the transcriptional induction of repair genes following replication blocks. This indicates that the non-catalytic FHA domain plays an important role in the transcriptional function of the Dun1p protein kinase.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Hammet A,Pike BL,Mitchelhill KI,Teh T,Kobe B,House CM,Kemp BE,Heierhorst J

doi

10.1016/s0014-5793(00)01392-2

keywords:

subject

Has Abstract

pub_date

2000-04-14 00:00:00

pages

141-6

issue

2-3

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(00)01392-2

journal_volume

471

pub_type

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