Abstract:
:Dun1p and Rad53p of the budding yeast Saccharomyces cerevisiae are members of a conserved family of cell cycle checkpoint protein kinases that contain forkhead-associated (FHA) domains. Here, we demonstrate that these FHA domains contain 130-140 residues, and are thus considerably larger than previously predicted by sequence comparisons (55-75 residues). In vivo, expression of the proteolytically defined Dun1p FHA domain, but not a fragment containing only the predicted domain boundaries, inhibited the transcriptional induction of repair genes following replication blocks. This indicates that the non-catalytic FHA domain plays an important role in the transcriptional function of the Dun1p protein kinase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Hammet A,Pike BL,Mitchelhill KI,Teh T,Kobe B,House CM,Kemp BE,Heierhorst Jdoi
10.1016/s0014-5793(00)01392-2keywords:
subject
Has Abstractpub_date
2000-04-14 00:00:00pages
141-6issue
2-3eissn
0014-5793issn
1873-3468pii
S0014-5793(00)01392-2journal_volume
471pub_type
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