Purification and characterization of mouse DNA polymerase alpha devoid of primase activity.

abstract：:The marked difference in auxin sensitivity between plant roots and shoots was studied in terms of auxin-induced stimulation of membrane P-type ATPase. The results suggest the existence in rice seedlings of, at least, two isoforms of soluble proteinous factors (SPF), SPF(I) and SPF(II), which are involved in the stimul...

journal_title：FEBS letters

authors： Kim D,Kim YS,Jung J

更新日期：1997-06-09 00:00:00

abstract：:The effects of anaphylatoxin C3a on human neutrophils were studied in comparison with C5a. Both peptides induced a transient shape change response and a respiratory burst. In both cases C3a was 50- to 100-times less potent than C5a. A marked chemotactic response with bimodal concentration dependence was obtained with ...

journal_title：FEBS letters

authors： Ehrengruber MU,Geiser T,Deranleau DA

更新日期：1994-06-13 00:00:00

abstract：:Transglutaminase 2 (TG2) is a GTP-binding protein with transglutaminase activity. Despite advances in the characterization of TG2 functions and their impact on cellular processes, the role of TG2 in Human chronic myelogenous leukemia K562 cell line is still poorly understood. To understand the biological significance ...

journal_title：FEBS letters

authors： Kang SK,Lee JY,Chung TW,Kim CH

更新日期：2004-11-19 00:00:00

abstract：:A possible interaction between tumor necrosis factor-alpha (TNF) and other cytokines/growth factors in stimulating the production of nerve growth factor (NGF) in Swiss 3T3 cells was studied. TNF's stimulatory activity on fibroblast NGF production was synergized by interleukin-1 alpha (IL-1 alpha), IL-1 beta and interf...

journal_title：FEBS letters

authors： Hattori A,Iwasaki S,Murase K,Tsujimoto M,Sato M,Hayashi K,Kohno M

更新日期：1994-03-07 00:00:00

abstract：:The MDM2 oncoprotein is overexpressed in many human tumors and cancers. MDM2 functions as an E3 ligase for p53 and for itself. MDM2 also interacts with the retinoblastoma protein (RB) and the transcription factor E2F1 to promote cell cycle S-phase entry. Here, we report that MDM2 protein expression is cell cycle-regul...

journal_title：FEBS letters

authors： Gu L,Ying H,Zheng H,Murray SA,Xiao ZX

更新日期：2003-06-05 00:00:00

abstract：:Thrombin is a multifunctional enzyme involved in coagulation, cell modulation and inflammation. We recently reported a novel membrane-associated prothrombin activator, abbreviated as MAPA, found in cultured fibroblasts and glial cell lines. In this study, we examined the physiological role of this enzyme. MAPA-like ac...

journal_title：FEBS letters

authors： Shikamoto Y,Shibusawa S,Okuyama I,Morita T

更新日期：1997-08-04 00:00:00

abstract：:The glucagon-like peptide 1 (7-37)/(7-36) amide (GLP-1) receptor belongs to a new subclass of seven transmembrane domain, G-protein coupled receptors comprising several receptors for peptide hormones. The receptors of this family share many common motifs including a relatively large N-terminal extracellular domain. Th...

journal_title：FEBS letters

authors： Wilmen A,Göke B,Göke R

更新日期：1996-11-25 00:00:00

abstract：:The role of Arp3 in mouse development was investigated utilizing a gene trap mutation in the Arp3 gene. Heterozygous Arp3(WT/GT) mice are normal, however, homozygous Arp3(GT/GT) embryos die at blastocyst stage. Earlier embryonic stages appear unaffected by the mutation, probably due to maternal Arp3 protein. Mutant bl...

journal_title：FEBS letters

authors： Vauti F,Prochnow BR,Freese E,Ramasamy SK,Ruiz P,Arnold HH

更新日期：2007-12-11 00:00:00

abstract：:NADP-malic enzyme (NADP-ME) is a widely distributed enzyme that catalyzes the oxidative decarboxylation of L-malate. Photosynthetic NADP-MEs are found in C4 bundle sheath chloroplasts and in the cytosol of CAM plants, while non-photosynthetic NADP-MEs are either plastidic or cytosolic in various plants. We propose a c...

journal_title：FEBS letters

authors： Drincovich MF,Casati P,Andreo CS

更新日期：2001-02-09 00:00:00

abstract：:The anomolous protein synthesis, sensitive to cycloheximide, which has been observed in brain mitochondrial and synaptosomal preparations, has been studied. It is concluded that this protein synthesis is due to the presence, as a contaminant in both preparations, of a ribosome-containing particle which contains solubl...

journal_title：FEBS letters

authors： Morgan IG

更新日期：1970-10-16 00:00:00

abstract：:The effect of the inositol phospholipid-binding antibiotic neomycin was studied on high-affinity GTPase in human platelet membranes. At low concentrations (up to 1 mM), neomycin by itself stimulated a high-affinity GTPase. This GTPase stimulation was additive with that caused by the hormonal factors, prostaglandin E1 ...

journal_title：FEBS letters

authors： Herrmann E,Jakobs KH

更新日期：1988-02-29 00:00:00

abstract：:We have investigated the transcriptional activity of human cytomegalovirus, herpes thymidine kinase, human chorionic gonadotropin alpha, somatostatin, immunoglobulin kappa chain, alpha crystallin, albumin and interferon-beta promoters in the fission yeast Schizosaccharomyces pombe. Among these, the human cytomegalovir...

journal_title：FEBS letters

authors： Toyama R,Okayama H

更新日期：1990-07-30 00:00:00

abstract：:We examined the effect of sodium butyrate on in vitro angiogenesis and cyclooxygenase (COX) expression using primary cultures of human intestinal microvascular endothelial cells (HIMEC). Butyrate inhibited VEGF-induced cellular proliferation, transmigration and tube formation of HIMEC. Butyrate also inhibited COX-2 ex...

journal_title：FEBS letters

authors： Ogawa H,Rafiee P,Fisher PJ,Johnson NA,Otterson MF,Binion DG

更新日期：2003-11-06 00:00:00

abstract：:The α-subunit of the cardiac voltage-gated sodium channel (NaV1.5) plays a central role in cardiomyocyte excitability. We have recently reported that NaV1.5 is post-translationally modified by arginine methylation. Here, we aimed to identify the enzymes that methylate NaV1.5, and to describe the role of arginine methy...

journal_title：FEBS letters

authors： Beltran-Alvarez P,Espejo A,Schmauder R,Beltran C,Mrowka R,Linke T,Batlle M,Pérez-Villa F,Pérez GJ,Scornik FS,Benndorf K,Pagans S,Zimmer T,Brugada R

更新日期：2013-10-01 00:00:00

abstract：:Three inositol-containing sialyloligosaccharides were isolated from normal human urine. Structural studies including gas-liquid chromatography of mono- and disaccharide derivatives, methylation analysis, mass spectrometry and glycosidase treatments indicated the structure NeuAc (alpha 2-3)Gal(beta 1-0)scyllo-inositol ...

journal_title：FEBS letters

authors： Parkkinen J

更新日期：1983-10-31 00:00:00

abstract：:The transcription factor Pdr1p recognizes Pdr1p/Pdr3p-response element (PDRE) to activate genes involved in multidrug resistance of the budding yeast. To identify novel targets of Pdr1p, we compared transcriptomes among the yeast cells bearing wild, disrupted and gain-of-function alleles of PDR1 using a high-throughpu...

journal_title：FEBS letters

authors： Miura F,Yada T,Nakai K,Sakaki Y,Ito T

更新日期：2001-09-07 00:00:00

abstract：:The internalisation of metabotropic glutamate receptor (mGluR1alpha) and its splice variant (mGluR1beta), in response to agonist and phorbol esters (PMA), has been studied. Both mGluR1alpha and mGluR1beta exhibit a similar rate of internalisation following PMA treatment, with a shift in their distribution from plasma ...

journal_title：FEBS letters

authors： Ciruela F,McIlhinney RA

更新日期：1997-11-24 00:00:00

abstract：:Incubation of rhodanese with hche aperonins GroEL and GroES (1:2 GroEL14:GroES7 molar ratio) under functional and steady state conditions for ATP leads to the formation of a high proportion of rhodanese-bound symmetric complexes (GroEL14(GroES7)2), as revealed by native electrophoresis. Aliquots of such samples were o...

journal_title：FEBS letters

authors： Llorca O,Marco S,Carrascosa JL,Valpuesta JM

更新日期：1997-03-24 00:00:00

abstract：:An electrometric technique was used to investigate electron transfer between spinach plastocyanin (Pc) and photooxidized primary electron donor P700 in photosystem I (PS I) complexes from the cyanobacterium Synechocystis sp. PCC 6803. In the presence of Pc, the fast unresolvable kinetic phase of membrane potential gen...

journal_title：FEBS letters

authors： Mamedov MD,Mamedova AA,Chamorovsky SK,Semenov AY

更新日期：2001-07-06 00:00:00

abstract：:Mammalian culture cells have the potential for periodicity, since high concentrations of serum can elicit the circadian expression of clock genes in rat-1 fibroblasts. However, the mechanism by which serum affects circadian gene expression remains unclear. In the present study, we incubated rat-1 cells with forskolin ...

journal_title：FEBS letters

authors： Yagita K,Okamura H

更新日期：2000-01-07 00:00:00

abstract：:Acceptor stem variants of tRNA(fMet) (Escherichia coli) have been characterized by nuclear magnetic resonance. The wild type contains a C1-A72 mismatch pair which is crucial for its biological function. For comparison, the mismatch was replaced by regular pairs U1-A72 and C1-G72. Further variants contain an altered di...

journal_title：FEBS letters

authors： Zuleeg T,Vogtherr M,Schübel H,Limmer S

更新日期：2000-04-28 00:00:00

abstract：:We investigated expression levels of Na(+),K(+)-ATPase alpha-isoforms and their ATPase activities in human colorectal cancer tissue and the accompanying normal mucosa. A decrease in expression of the alpha1-isoform protein was observed in all sampled cancer tissues compared with the normal mucosae. The level of ouabai...

journal_title：FEBS letters

authors： Sakai H,Suzuki T,Maeda M,Takahashi Y,Horikawa N,Minamimura T,Tsukada K,Takeguchi N

更新日期：2004-04-09 00:00:00

abstract：:Macrophages contain arginase and an inducible NO synthase, demonstrated by using L-arginine, the common substrate, for production of both nitric oxide and urea. Arginase was inhibited by nitrite, the stable end product of NO. This inhibition was non-competitive, and could not be explained by the reaction of nitrite wi...

journal_title：FEBS letters

authors： Hrabák A,Bajor T,Temesi A,Mészáros G

更新日期：1996-07-22 00:00:00

abstract：:Osteoblastic cells have been shown to be involved in osteoclast formation through cell to cell contacts. This study was designed to examine the possible function of vascular cell adhesion molecule 1 (VCAM-1) during osteoclastogenesis. As a source for stromal cells we used the recently established mouse bone marrow str...

journal_title：FEBS letters

authors： Feuerbach D,Feyen JH

更新日期：1997-01-27 00:00:00

abstract：:The conversion of the cellular prion protein into the beta-sheet-rich scrapie prion protein is thought to be the key step in the pathogenesis of prion diseases. To gain insight into this structural conversion, we analyzed the intrinsic structural propensity of the amino acid sequence of the murine prion C-terminal dom...

journal_title：FEBS letters

authors： Jamin N,Coïc YM,Landon C,Ovtracht L,Baleux F,Neumann JM,Sanson A

更新日期：2002-10-09 00:00:00

abstract：:The pluripotent nature of human embryonic stem cells (hESC) has attracted great interest in using them as a source of cells or tissue in cell therapy. However, in order to be used in regenerative medicine, the pluripotent hESC lines should be established and propagated according to good manufacturing practice quality ...

journal_title：FEBS letters

authors： Skottman H,Dilber MS,Hovatta O

更新日期：2006-05-22 00:00:00

abstract：:Tripeptidyl peptidase I (TPP-I) is a lysosomal enzyme that cleaves tripeptides from the N-terminus of polypeptides. A comparison of TPP-I amino acid sequences with sequences derived from an EST database suggested that TPP-I is identical to a pepstatin-insensitive carboxyl proteinase of unknown specificity which is mut...

journal_title：FEBS letters

authors： Vines DJ,Warburton MJ

更新日期：1999-01-25 00:00:00

abstract：:Transcription mediators are evolutionarily conserved from yeast to human. We previously reported the specific in vivo roles of mediators during development. Transcriptional mediators including med-6, med-7, and med-10 were shown to be involved in the regulated transcription of specific genes, but not in the transcript...

journal_title：FEBS letters

authors： Kwon JY,Kim-Ha J,Lee BJ,Lee J

更新日期：2001-11-23 00:00:00

abstract：:Peroxisome proliferator-activated receptor gamma (PPARgamma) ligands have been shown to inhibit the effects of proinflammatory cytokines such as interleukin-1beta (IL-1beta). This cytokine plays a key role in articular pathophysiologies by inducing the production of inflammatory mediators such as nitric oxide (NO) and...

journal_title：FEBS letters

authors： Boyault S,Bianchi A,Moulin D,Morin S,Francois M,Netter P,Terlain B,Bordji K

更新日期：2004-08-13 00:00:00

abstract：:Using radiolysis with (32)P enriched phosphate as an internal source of ionizing radiation, the formation of hydroperoxo-ferric complex from oxy-ferrous precursor with a high yield was monitored at 77 K in heme oxygenase (HO) by means of optical absorption spectroscopy. Well-resolved absorption spectra (maxima at 421 ...

journal_title：FEBS letters

authors： Denisov IG,Ikeda-Saito M,Yoshida T,Sligar SG

更新日期：2002-12-04 00:00:00