Abstract:
:Transcription mediators are evolutionarily conserved from yeast to human. We previously reported the specific in vivo roles of mediators during development. Transcriptional mediators including med-6, med-7, and med-10 were shown to be involved in the regulated transcription of specific genes, but not in the transcription of ubiquitous genes. In this report we have identified and characterized the Caenorhabditis elegans med-7 gene. A genetic mutation in the med-7 gene was identified by comparing genetic and physical maps and determining the molecular lesion. let-49 was found to have a nonsense mutation in the coding region of the med-7 gene. The identification of let-49 as the med-7 gene was confirmed by rescue experiments. The phenotype of the let-49 mutation indicated that the med-7 gene is required for normal postembryonic development. RNAi experiments showed that med-7 is also involved in embryogenesis and the gonad and germ cell development.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kwon JY,Kim-Ha J,Lee BJ,Lee Jdoi
10.1016/s0014-5793(01)03072-1keywords:
subject
Has Abstractpub_date
2001-11-23 00:00:00pages
305-8issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(01)03072-1journal_volume
508pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::The addition of 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) to peroxisomes decreased the production of acid-soluble radioactivity formed by beta-oxidation of [1-(14)C]arachidonate due to substrate removal by esterification into the acceptor. This peroxisomal-associated acyl-CoA:1-acyl-GPC acyltransferase activity ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00565-q
更新日期:1995-06-26 00:00:00
abstract::The cytosolic lipid peroxidation product 4-hydroxynonenal (HNE) is rapidly metabolized in mitochondria isolated from rat kidney cortex. About 80% of HNE was degraded within 3 min of incubation. Main products of HNE which were identified in mitochondria were the hydroxynonenoic acid, the 1,4-dihydroxynonene and the glu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00922-8
更新日期:1994-09-19 00:00:00
abstract::Human Tid-1 (hTid-1) is a DnaJ chaperone protein with homology to the Drosophila tumor suppressor Tid56. We report the first case of a tumor-associated mutation at the human TID1 locus, which was identified in the SF767 glioma cell line giving rise to aberrantly high levels of a hTid-1(L) mutant variant. In this study...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.11.034
更新日期:2004-12-17 00:00:00
abstract::The Ca(2+)-activated photoprotein, aequorin, contains six tryptophan residues and has a bioluminescence emission maximum at 465 nm. On converting the six tryptophan residues to phenylalanine, the mutant aequorins exhibited varied luminescence activities and spectra, but one mutant, with tryptophan-86 replaced by pheny...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81247-j
更新日期:1992-04-20 00:00:00
abstract::Endothelial cells play a pivotal role in the development of atherosclerosis. An 'activated' phenotype of these cells is manifested by signal transduction-dependent expression of genes encoding cytokines, pro- and anticoagulant factors, and cell adhesion molecules. In the current study we examined the effect of ouabain...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01301-6
更新日期:1995-12-11 00:00:00
abstract::PA28, a 200 kDa activator of 20S proteasomes, was purified from human placenta and was gel electrophoretically resolved into two different subunits, alpha and beta. In reconstitution experiments, alpha-subunits alone were found to re-associate forming homooligomers with an M(r) of about 200 kDa, which elicit a stimula...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00946-5
更新日期:1996-09-30 00:00:00
abstract::Ischemic preconditioning (IPrec) improves post-ischemic dysfunctions of the myocardium along with activation of protein kinase C isozymes including PKCdelta. Moreover, expression of cardio-protective determinants can reduce ischemic damages. Because IPrec is limited in aged hearts, we assessed in an experimental model...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)01342-5
更新日期:2003-12-18 00:00:00
abstract::An approach is presented for the determination of the branch location of 1 or 2 extra N-acetyllactosamine units in sialo N-linked carbohydrate chains from glycoproteins. Tetraantennary oligosaccharides containing extra N-acetyllactosamine units were digested with endo-beta-galactosidase, followed by treatment with N-a...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80931-r
更新日期:1991-07-29 00:00:00
abstract::Crystals of methylamine-treated alpha 2-macroglobulin (alpha 2M-MA), alpha 2-macroglobulin in complex with two molecules of trypsin, alpha 2M-T2, one molecule of plasmin, alpha 2M-PL, and one molecule of plasmin followed by methylamine-treatment, alpha 2M-PL(MA), have reproducibly been obtained using ammonium sulfate ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80881-3
更新日期:1991-11-04 00:00:00
abstract::The heat shock factor (HSF1) is the central regulator of the heat stress (hs) response and is required for stimulating the transcription of the hs genes and consequently the expression of heat shock proteins. To promote the polymerase II-dependent transcription of the hs genes, HSF has to communicate with the basal tr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01152-1
更新日期:1998-10-09 00:00:00
abstract::5(S)-Hydroxyicosatetraenoate stimulates human polymorphonuclear neutrophils (PMNs) to raise their cytosolic calcium. It also potentiates the cells' degranulation responses to platelet-activating factor and diacylglycerols. We synthesized 5(R)-hydroxyicosatetraenoate and found it to be 20-100-fold weaker than the natur...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80360-0
更新日期:1988-11-21 00:00:00
abstract::Various vertebrates use ultraviolet (UV) vision for such basic behaviors as mating, foraging, and predation. We have successfully interchanged the color-sensitivities of the mouse UV pigment and the human blue pigment by introducing forward and reverse mutations at five sites. This unveils for the first time the gener...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02269-9
更新日期:2000-12-08 00:00:00
abstract::A steady-state framework was applied to the ubiquitous tricyclic enzyme cascade structure, as seen in the mitogen-activated protein (MAP) kinase system, to analyze the effect of upstream kinase concentrations on final output response. The results suggest that signal amplification achieved by the cascade structure ensu...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/S0014-5793(04)00021-3
更新日期:2004-01-30 00:00:00
abstract::Under physiological conditions significant amounts of low-density lipoprotein LDL particles ar taken up by cells independently of specific high-affinity LDL receptors (apo-B receptors). Previously it was established that some cells contain surface sites capable of binding liposomes. We proposed that liposome-binding s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80006-o
更新日期:1991-08-05 00:00:00
abstract::NELL1 is an extracellular protein inducing osteogenic differentiation and bone formation of osteoblastic cells. To elucidate the intracellular signaling cascade evoked by NELL1, we have shown that NELL1 protein transiently activates the MAPK signaling cascade, induces the phosphorylation of Runx2, and promotes the rap...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.12.006
更新日期:2008-01-23 00:00:00
abstract::Chromogranin A is known to undergo pH induced conformational changes, and the difference in conformation is supposed to be responsible for the difference in Ca2+ binding property. To gain insight regarding the overall structure and the nature of pH-induced conformational changes of chromogranin A, limited trypsin dige...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80715-7
更新日期:1993-11-22 00:00:00
abstract::The classical short-term effect (within minutes) of arginine vasopressin (AVP) consists in increasing sodium, chloride and water transport in kidney cells. More recently, long-term actions (several hours) of the hormone have been evidenced on water and sodium fluxes, due to transcriptional enhancement in the expressio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01408-8
更新日期:1999-11-05 00:00:00
abstract::In rat cerebrocortical synaptosomes, the addition of 4 beta-phorbol dibutyrate (4 beta-PDBu) and arachidonic acid enhances and decreases, respectively, the glutamate release evoked by 4-aminopyridine. Pretreatment of synaptosomes with 12-O-tetradecanoylphorbol 13-acetate (TPA) or pre-incubation with staurosporine, pre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80312-5
更新日期:1992-01-27 00:00:00
abstract::A 569 bp probe against the beta-chain of hepatotropin was used to examine expression of RNA for this growth factor in human adult and foetal liver, foetal kidney and pancreas, and rat liver after partial hepatectomy. Low level expression of a 6 kb RNA occurred in human adult and normal rat liver. 70% hepatectomy incre...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81239-k
更新日期:1990-09-17 00:00:00
abstract::SUPERMAN was identified as a putative regulator of transcription that acts in floral development, but its function remains to be clarified. We demonstrate here that SUPERMAN is an active repressor whose repression domain is located in the carboxy-terminal region. Ectopic expression of SUPERMAN that lacked the repressi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02435-3
更新日期:2002-03-13 00:00:00
abstract::Hematopoietic progenitors have been shown to retain plasticity and switch lineages by appropriate stimuli. However, mature blood cells hardly showed such differentiation plasticity. In this paper, we tried to reprogram mature B cells into erythroid lineage by expressing various hematopoietic transcription factors. Amo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2012.08.019
更新日期:2012-10-19 00:00:00
abstract::The existence of the internal promoter Pi in the 16 S/23 S intergenic spacers of the rRNA operons of an eubacterium Escherichia coli and archaebacterium Halobacterium halobium is proposed. The possible functional significance of these promoters is discussed. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80233-8
更新日期:1987-07-27 00:00:00
abstract::Yeasts with disruptions in the genes PYC1 and PYC2 encoding the isoenzymes of pyruvate carboxylase cannot grow in a glucose-ammonium medium (Stucka et al. (1991) Mol. Gen. Genet. 229, 307-315). We have isolated a dominant mutation, BPC1-1, that allows growth in this medium of yeasts with interrupted PYC1 and PYC2 gene...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)01337-7
更新日期:1995-12-18 00:00:00
abstract::The action of substrate analogs containing the tetrazolyl group instead of the C-terminal carboxy group on the peptidase activity of carboxypeptidase A is studied. The analogs compete with the substrate for the secondary binding site thus showing activation phenomena. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81561-2
更新日期:1987-02-09 00:00:00
abstract::Purified plasma membrane vesicles isolated from multidrug-resistant human KB-V1 cells accumulate [3H]vinblastine in an energy-dependent manner. The accumulation of [3H]vinblastine in the presence of ATP is a saturable process. ATP can be replaced by other purine nucleotide triphosphates, of which GTP is the most effic...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80632-q
更新日期:1992-06-15 00:00:00
abstract::AoXlnR was isolated as a transcriptional activator of the major xylanase gene, xynF1, in Aspergillus oryzae. To investigate the spectrum of genes under the control of AoXlnR, expression of the xylanolytic and cellulolytic genes in an A. oryzae wild type strain, an AoxlnR disruptant and an AoXlnR overexpressed strain w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03328-8
更新日期:2002-09-25 00:00:00
abstract::The ∆Np63 isoform of the p53-family transcription factor Trp63 is a key regulator of mammary epithelial stem cells that is involved in breast cancer development. To investigate the role of ∆Np63 at different stages of normal mammary gland development, we generated a ∆Np63-inducible conditional knockout (cKO) mouse mod...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13703
更新日期:2020-03-01 00:00:00
abstract::A covalent complex formed by bacterial tRNAs and prothymosin alpha, an abundant acidic nuclear protein involved in proliferation of mammalian cells, upon production of the recombinant rat protein in Escherichia coli cells was studied. Several tRNA attachment sites were identified in the prothymosin alpha molecule usin...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00580-3
更新日期:1999-05-21 00:00:00
abstract::The two subunits of gamma-glutamyl transpeptidase (EC 2.3.2.2) are derived from a single-chain glycosylated precursor. A small fraction of the propeptide survives proteolytic processing in the rat kidney and has been purified by an immunoaffinity technique. The propeptide contains determinants for both the subunits an...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80046-1
更新日期:1986-01-01 00:00:00
abstract::The informational content of the primary structure of thymopoietin (TP) is investigated using the informational spectrum method (ISM). We show that the sequence of TP shares common information with the sequences of long postsynaptic snake neurotoxins, although no apparent similarity was found among their primary struc...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80561-1
更新日期:1988-10-24 00:00:00