Reconstitution of proteasome activator PA28 from isolated subunits: optimal activity is associated with an alpha,beta-heteromultimer.

abstract：:We have purified a 38 kDa protein from bovine brain which is cross-reactive with an affinity purified antibody against the 35 kDa phosphatidylinositol transfer protein from the same source. Controlled trypsinization of the 38 kDa protein yielded an immunoreactive protein of 35 kDa which displayed a 6-fold increase in ...

journal_title：FEBS letters

authors： van den Akker WM,Westerman J,Gadella TW Jr,Wirtz KW,Snoek GT

更新日期：1990-12-10 00:00:00

abstract：:(7-methoxycoumarin-4-yl)Acetyl-Pro-Leu-Gly-Leu-(3-[2,4-dinitrophenyl]-L- 2,3-diaminopropionyl)-Ala-Arg-NH2 (Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2) has been synthesised as a fluorogenic substrate for the matrix metalloproteinases. The highly fluorescent 7-methoxycoumarin group is efficiently quenched by energy transfer t...

journal_title：FEBS letters

authors： Knight CG,Willenbrock F,Murphy G

更新日期：1992-01-27 00:00:00

abstract：:Chlorella virus-encoded membrane protein Kcv represents a new class of potassium channel. This 94-amino acids miniature K(+) channel consists of two trans-membrane alpha-helix domains intermediated by a pore domain that contains a highly conserved K(+) selectivity filter. Therefore, as an archetypal K(+) channel, the ...

journal_title：FEBS letters

authors： Shim JW,Yang M,Gu LQ

更新日期：2007-03-06 00:00:00

abstract：:Mutual arrangement of histone H1 molecules in chromatin extended in low salt-EDTA buffer and additionally in the presence of urea was studied by means of reversible cross-linking combined with chymotryptic digestion. In the chromatins tested, the chymotryptic halves of H1 were cross-linked in all possible combinations...

journal_title：FEBS letters

authors： Nikolaev LG,Glotov BO,Dashkevich VK,Barbashov SF,Severin ES

更新日期：1983-10-31 00:00:00

abstract：:Signaling via G-protein coupled receptors is initiated by receptor-catalyzed nucleotide exchange on Galpha subunits normally bound to GDP and Gbetagamma. Activated Galpha . GTP then regulates effectors such as adenylyl cyclase. Except for Gbetagamma, no known regulators bind the adenylyl cyclase-stimulatory subunit Ga...

journal_title：FEBS letters

authors： Johnston CA,Ramer JK,Blaesius R,Fredericks Z,Watts VJ,Siderovski DP

更新日期：2005-10-24 00:00:00

abstract：:Only recently have products of the enzymatic breakdown of the chlorophylls been characterized for the first time. All catabolites isolated until now from a chlorophyte and from angiosperms have in common the position at which the primary oxygenative ring cleavage occurs, yielding linear tetrapyrroles (19-formyl-1[21H,...

journal_title：FEBS letters

authors： Curty C,Engel N,Gossauer A

更新日期：1995-05-01 00:00:00

abstract：:Bacterial DNA supercoiling is controlled by balancing the supercoiling activity of DNA gyrase and the relaxing activity of DNA topoisomerase I. We have characterized the gyrB gene from a top A deletion mutant of Escherichia coli (DM800) that has a compensatory mutation in gyrB, lowering the activity of gyrase 10-fold,...

journal_title：FEBS letters

authors： McEachern F,Fisher LM

更新日期：1989-08-14 00:00:00

abstract：:The cytochrome bc(1) complex catalyzes electron transfer from ubiquinol to cytochrome c by a protonmotive Q cycle mechanism in which electron transfer is linked to proton translocation across the inner mitochondrial membrane. In the Q cycle mechanism proton translocation is the net result of topographically segregated...

journal_title：FEBS letters

authors： Hunte C,Palsdottir H,Trumpower BL

更新日期：2003-06-12 00:00:00

abstract：:Benzimidazoles are widely used as anthelmintic agents and systemic fungicides. In susceptible organisms, benzimidazoles bind to beta-tubulin and block microtubule polymerization. To further characterize this interaction, site-directed mutagenesis followed by gene replacement was used to change Saccharomyces cerevisiae...

journal_title：FEBS letters

authors： Li J,Katiyar SK,Edlind TD

更新日期：1996-04-29 00:00:00

abstract：:Recombinant Plasmodium falciparum glyoxalase I (PfGlx I) was characterized as monomeric Zn(2+)-containing enzyme of 44 kDa. The K(M) value of the methylglyoxal-glutathione adduct is 77+/-15 microM, the k(cat) value being 4000 min(-1) at 25 degrees C and pH 7.0. PfGlx I consists of two halves, each of which is homologo...

journal_title：FEBS letters

authors： Iozef R,Rahlfs S,Chang T,Schirmer H,Becker K

更新日期：2003-11-20 00:00:00

abstract：:The effect of platelet-derived growth factor (PDGF) on Rap1 expression was investigated in rat vascular smooth muscle cells (SMC). First, evidence for Rap1 proteins was shown by their: (i) detection in membranes using a specific anti-Rap1 antibody, (ii) typical shift in electrophoretic mobility as a consequence of red...

journal_title：FEBS letters

authors： Quarck R,Bryckaert M,Magnier C,Corvazier E,Bredoux R,de Gunzburg J,Fontenay M,Tobelem G,Enouf J

更新日期：1994-04-04 00:00:00

abstract：:Treatment of MCF-7 breast cancer cells with 50 nM okadaic acid triggers an apoptotic response which is accompanied by a 7-fold increase in the activity of a protein kinase with a relative molecular mass of 53 kDa. The activity of the kinase was stimulated by cell treatment with inhibitors of phosphoprotein phosphatase...

journal_title：FEBS letters

authors： Rossini GP,Pinna C,Viviani R

更新日期：1997-06-30 00:00:00

abstract：:The plant-specific GAI-RGA-and-SCR (GRAS) family of proteins function as transcriptional regulators and play critical roles in development and signalling. Recent structural studies have shed light on the molecular functions at the structural level. The conserved GRAS domain comprises an α-helical cap and α/β core subd...

journal_title：FEBS letters

authors： Hakoshima T

更新日期：2018-02-01 00:00:00

abstract：:The complexity of the proteasome gene family in higher plants was investigated by identification and sequencing cDNA clones from the Arabidopsis thaliana database showing homologies to 20S proteasome subunits. We identified plant counterparts for each of the 14 proteasomal subunit subfamilies. Moreover, several of the...

journal_title：FEBS letters

authors： Parmentier Y,Bouchez D,Fleck J,Genschik P

更新日期：1997-10-27 00:00:00

abstract：:1,2-Didecanoylglycerol (diC10) is taken up by human platelets and sequentially converted to 1,2-didecanoylphosphatidic acid (PA10) and 1,2-didecanoylphosphatidylinositol (PI10). Agents that increase cyclic AMP in platelets, such as prostacyclin and forskolin, sequentially convert diC10 to PA10 and PI10. They decrease ...

journal_title：FEBS letters

authors： Lapetina EG

更新日期：1986-01-20 00:00:00

abstract：:The structure of the complex of Ras with the Ras-binding domain of its effector RalGDS (RGS-RBD), the first genuine Ras-effector complex, has been solved by X-ray crystallography. As with the Rap-RafRBD complex (Nasser et al., 1995), the interaction is via an inter-protein beta-sheet between the switch I region of Ras...

journal_title：FEBS letters

authors： Vetter IR,Linnemann T,Wohlgemuth S,Geyer M,Kalbitzer HR,Herrmann C,Wittinghofer A

更新日期：1999-05-21 00:00:00

abstract：:Dictyostelium discoideum Ax2 produces both L-erythro-tetrahydrobiopterin (BH4) and its stereoisomer D-threo-BH4 (DH4). The putative cofactor function of them for phenylalanine hydroxylase (PAH) was investigated through genetic manipulation and quantitative determination of pteridines. In addition to establishing that ...

journal_title：FEBS letters

authors： Kim HL,Park MB,Park YS

更新日期：2011-10-03 00:00:00

abstract：:The non-selective gastrin/cholecystokinin receptor antagonists proglumide and benzotript inhibit colon carcinoma cell proliferation by binding to the 78 kDa gastrin-binding protein (GBP) (Baldwin, Proc. Natl. Acad. Sci. USA, 91 (1994) 7593-7597). However, although most colon carcinoma cell lines synthesize progastrin,...

journal_title：FEBS letters

authors： Baldwin GS

更新日期：1995-02-06 00:00:00

abstract：:A novel potassium channel beta-subunit (Kv beta 3) was cloned from rat brain being the third member of a Kv beta subunit gene family. It is a protein of 403 amino acid residues with a 68% amino acid sequence homology to Kv beta 1.1. Kv beta 3 is primarily expressed in rat brain having a distribution distinct to those ...

journal_title：FEBS letters

authors： Heinemann SH,Rettig J,Wunder F,Pongs O

更新日期：1995-12-27 00:00:00

abstract：:Endoplasmic reticulum stress (ER-stress) is associated with ataxia telangiectasia mutated (ATM) gene. We present here conclusive data showing that ATM blocks ER-stress induced by tunicamycin or ionizing radiation (IR). X-box protein-1 (XBP-1) splicing, GRP78 expression and caspase-12 activation were increased by tunic...

journal_title：FEBS letters

authors： He L,Kim SO,Kwon O,Jeong SJ,Kim MS,Lee HG,Osada H,Jung M,Ahn JS,Kim BY

更新日期：2009-03-04 00:00:00

abstract：:Extracellular laccase (benzenediol: oxygen oxidoreductase EC 1.10.3.2) from the lignin-degrading fungus, Phlebia radiata, was shown to contain a novel combination of electron carriers as its prosthetic groups. In addition to two copper atoms per enzyme molecule, one molecule of PQQ was included as a cofactor. The EPR ...

journal_title：FEBS letters

authors： Karhunen E,Niku-Paavola ML,Viikari L,Haltia T,van der Meer RA,Duine JA

更新日期：1990-07-02 00:00:00

abstract：:A conformational transition between E2 and E1 forms of Na, K-ATPase induced by different nucleotides has been studied under steady state conditions using the enzyme labelled with 5-iodoacetamidofluorescein. In the presence of K+ the plot of fluorescence as a function of [ATP], [ADP] or [CTP] (in a range of 5 microM-12...

journal_title：FEBS letters

authors： Lopina OD,Pindel EV,Boldyrev AA

更新日期：1990-06-18 00:00:00

abstract：:2-thiouracil (TU), an established antithyroid drug and melanoma-seeker, was found to selectively inhibit neuronal nitric oxide synthase (nNOS) in a competitive manner (K(i)=20 microM), being inactive on the other NOS isoforms. The drug apparently interfered with the substrate- and tetrahydrobiopterin (BH(4))-binding t...

journal_title：FEBS letters

authors： Palumbo A,d'Ischia M,Cioffi FA

更新日期：2000-11-24 00:00:00

abstract：:G-quadruplex ligands DODC and TMPyP4 have different binding modes to quadruplex structure and cause cell proliferation arrest. Here we showed that DODC was more efficient in cell growth inhibition than TMPyP4. Both G-quadruplex ligands induced nuclear-cytoplasmic shuttling and accumulation of TERT in mitochondria. Thi...

journal_title：FEBS letters

authors： Zhuang XY,Yao YG

更新日期：2013-06-05 00:00:00

abstract：:Holocytochrome c synthase (HCCS) attaches heme covalently to mitochondrial respiratory cytochromes c. Little is known about the reaction of heme attachment to apocytochromes c by HCCS, although recently it has been established that the CXXCH motif and the N-terminus of the apocytochrome polypeptide are important prote...

journal_title：FEBS letters

authors： Zhang Y,Stevens JM,Ferguson SJ

更新日期：2014-09-17 00:00:00

abstract：:Magnesium (Mg(2+)) transport across membranes plays an essential role in cellular growth and survival. TRPM7 is the unique fusion of a Mg(2+) permeable pore with an active cytosolic kinase domain, and is considered a master regulator of cellular Mg(2+) homeostasis. We previously found that the genetic deletion of TRPM...

journal_title：FEBS letters

authors： Deason-Towne F,Perraud AL,Schmitz C

更新日期：2011-07-21 00:00:00

abstract：:Binding of cysteine proteinases of the papain superfamily (papain and cathepsins B, B-like and L) to basement membranes was studied by using the enzymatic activity of these proteinases against their specific fluorogenic substrates. Papain inactivated by E64 was used for Kd determination by competition experiments. The...

journal_title：FEBS letters

authors： Guinec N,Dalet-Fumeron V,Pagano M

更新日期：1992-08-24 00:00:00

abstract：:Structure/function relationships in troponin C are studied with vertebrate fast-twitch fibers by exchanging the skeletal troponin C with two bacterially synthesized recombinant proteins designed by site-directed mutagenesis of cardiac troponin C. One mutant (CBM1) contained an additional active site, by deleting Val-2...

journal_title：FEBS letters

authors： Gulati J,Babu A,Putkey JA

更新日期：1989-05-08 00:00:00

abstract：:We have cloned a novel nuclear gene for a ribosomal protein of rice and Arabidopsis that is like the bacterial ribosomal protein S9. To determine the subcellular localization of the gene product, we fused the N-terminal region and green fluorescent protein and expressed it transiently in rice seedlings. Localized fluo...

journal_title：FEBS letters

authors： Arimura S,Takusagawa S,Hatano S,Nakazono M,Hirai A,Tsutsumi N

更新日期：1999-05-07 00:00:00

abstract：:Antithrombin III Hamilton is a structural variant of antithrombin III (AT-III) with normal heparin affinity but impaired serine protease inhibitory activity. The molecular defect of AT-III-Hamilton is a substitution of threonine for alanine at amino acid residue 382. Recently it has been shown that both plasma-derived...

journal_title：FEBS letters

authors： Austin RC,Rachubinski RA,Blajchman MA

更新日期：1991-03-25 00:00:00