Abstract:
:Dictyostelium discoideum Ax2 produces both L-erythro-tetrahydrobiopterin (BH4) and its stereoisomer D-threo-BH4 (DH4). The putative cofactor function of them for phenylalanine hydroxylase (PAH) was investigated through genetic manipulation and quantitative determination of pteridines. In addition to establishing that dihydropteridine reductase (DHPR) and dihydrofolate reductase (DHFR) constitute the regeneration pathway of both BH4 and DH4, the results suggested that BH4 is a preferential cofactor for PAH in vivo, not a secondary product of DH4, which functions mainly as an antioxidant. Our result also demonstrated that PAH may be essential for Dictyostelium growth in nature, and thus it appears that the organism has evolved a strategy to maintain BH4 level via regeneration pathway at the expense of DH4 under oxidative stress conditions.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kim HL,Park MB,Park YSdoi
10.1016/j.febslet.2011.08.026subject
Has Abstractpub_date
2011-10-03 00:00:00pages
3047-51issue
19eissn
0014-5793issn
1873-3468pii
S0014-5793(11)00628-4journal_volume
585pub_type
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