Abstract:
:The domains of the pore-forming protein responsible for the gate and channel formations were separated and identified in the outer membrane of Pseudomonas aeruginosa. The proteolytic cleavage of the 46K channel protein, protein D2, yielded two major domains with apparent M(r) of 27K and 19K. We identified the 27K polypeptide to be the channel-forming domain by an in vitro permeability assay. The channel size of purified 27K domain was indistinguishable from that of native protein D2. Degradation of the 19K domain into small subfragments increases the channel activity about ten times suggesting that the 19K polypeptide forms the gate or cap.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Yoshihara E,Nakae Tdoi
10.1016/0014-5793(92)80826-3keywords:
subject
Has Abstractpub_date
1992-07-13 00:00:00pages
5-8issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(92)80826-3journal_volume
306pub_type
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