Abstract:
:A comparison is made between the PQA----P+QA- and PQAQB----P+QAQB-transitions in Rps. viridis and Rb. sphaeroides reaction centers (RCs) by the use of light-induced Fourier transform infrared (FTIR) difference spectroscopy. In Rb. sphaeroides RCs, we identify a signal at 1650 cm-1 which is present in the P+QA-minus-PQA spectrum and not in the P+QAQB(-)-minus-PQAQB spectrum. In contrast, this signal is present in both P+QA(-)-minus-PQA- and P+QAQB(-)-minus-PQAQB spectra of Rps. viridis RCs. These data are interpreted in terms of a conformational change of the protein backbone near QA (possible at the peptide C = O of a conserved alanine residue in the QA pocket) and of the different bonding interactions of QB with the protein in the RC of the two species.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Nabedryk E,Bagley KA,Thibodeau DL,Bauscher M,Mäntele W,Breton Jdoi
10.1016/0014-5793(90)81506-jsubject
Has Abstractpub_date
1990-06-18 00:00:00pages
59-62issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(90)81506-Jjournal_volume
266pub_type
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