The structural elements of hirudin which bind to the fibrinogen recognition site of thrombin are exclusively located within its acidic C-terminal tail.

abstract：:Adenosine and ATP modulate cellular and tissue functions via specific P1 and P2 receptors, respectively. Although, in general, adenosine inhibits excitability and ATP functions as an excitatory transmitter in the central nervous system, little is known about the direct interaction between P1 and P2 receptors. We recen...

journal_title：FEBS letters

authors： Yoshioka K,Hosoda R,Kuroda Y,Nakata H

更新日期：2002-11-06 00:00:00

abstract：:Heat shock (44 degrees C for 10 min) or ATP depletion by an uncoupler (CCCP for 20 min) is shown to result in stimulation of hsp68/70 synthesis in Ehrlich tumor cells. After 3 h of recovery, the cells become thermotolerant. Surprisingly, repeated ATP depletion caused by CCCP or rotenone (a respiratory inhibitor) treat...

journal_title：FEBS letters

authors： Gabai VL,Kabakov AE

更新日期：1993-08-02 00:00:00

abstract：:The high cGMP sensitivity of cAMP-dependent protein kinase A (type II) (PKAII) from invertebrates led to the hypothesis that cGMP directly activates PKAII under physiological conditions. We tested this idea using PKAII holoenzyme purified from the honeybee brain in an assay with short stimulation times. In the presenc...

journal_title：FEBS letters

authors： Leboulle G,Müller U

更新日期：2004-10-08 00:00:00

abstract：:The fluorescent Ca2+ indicator Fura 2 was used to monitor Ca2+ release induced by the Ins(1,4,5)P3-mobilizing agonist angiotensin II (Ag II), caffeine and 2,5-di-(tert-butyl)-1,4-benzohydroquinone (tuBHQ), in intact bovine adrenal chromaffin cells. Under low external Ca2+ conditions, tuBHQ, Ag II and caffeine elicited...

journal_title：FEBS letters

authors： Robinson IM,Burgoyne RD

更新日期：1991-09-09 00:00:00

abstract：:The complete amino acid sequence of porcine muscarinic acetylcholine receptor III has been deduced by cloning and sequencing the genomic DNA. The antagonist binding properties of muscarinic acetylcholine receptor III expressed from the cloned DNA in Xenopus oocytes correspond most closely to those of the pharmacologic...

journal_title：FEBS letters

authors： Akiba I,Kubo T,Maeda A,Bujo H,Nakai J,Mishina M,Numa S

更新日期：1988-08-01 00:00:00

abstract：:Isoenzymes of creatine kinase (CK, EC 2.7.3.2) in guinea-pig smooth, cardiac and skeletal muscles as well as in brain were analyzed by cellulose acetate electrophoresis and FPLC gel permeation chromatography. In crude tissue extracts of smooth muscles brain type BB-CK and the hybrid form MB-CK were detected, but in en...

journal_title：FEBS letters

authors： Ishida Y,Wyss M,Hemmer W,Wallimann T

更新日期：1991-05-20 00:00:00

abstract：:Incubation of rat adipocytes with the phorbol ester TPA caused a dose-dependent, persistent increase in phosphatidate phosphohydrolase activity and decrease in fatty acyl-CoA synthetase activity. Half-maximal effects were obtained with 1-2 X 10(-10) M TPA. TPA did not alter basal or noradrenaline-stimulated lipolysis....

journal_title：FEBS letters

authors： Hall M,Taylor SJ,Saggerson ED

更新日期：1985-01-07 00:00:00

abstract：:T cells express two different types of voltage-independent Ca(2+)-activated K(+) channels with small (SK) and intermediate (IK) conductance that serve important roles in the activation of T lymphocytes. In contrast to the IK channels from T lymphocytes which are upregulated upon mitogen stimulation, SK channels of Jur...

journal_title：FEBS letters

authors： Jäger H,Adelman JP,Grissmer S

更新日期：2000-03-10 00:00:00

abstract：:Vasopressin elicited a dose-dependent inhibition of glucagon-induced cAMP accumulation in isolated hepatocytes. This response was not diminished by incubation of cells with the calmodulin antagonists trifluoperazine or chlorpromazine and was only slightly reduced in Ca2+-depleted hepatocytes. Half-maximal inhibition o...

journal_title：FEBS letters

authors： Morgan NG,Shipp CC,Exton JH

更新日期：1983-11-14 00:00:00

abstract：:Immature Drosophila rhodopsin is N-glycosylated, but undergoes complete deglycosylation during the process of protein maturation. In order to elucidate the site of glycosylation and its role in rhodopsin synthesis, we investigated the in vitro and in vivo synthesis of rhodopsin whose putative N-glycosylation sites (As...

journal_title：FEBS letters

authors： Katanosaka K,Tokunaga F,Kawamura S,Ozaki K

更新日期：1998-03-13 00:00:00

abstract：:Plasma membrane, sarco-endoplasmic reticulum and secretory pathway Ca2+-ATPases (designated PMCA, SERCA and SPCA) regulate intracellular Ca2+ in animal cells. The presence of PMCA, and the absence of SERCA, in sea urchin sperm is known. By using inhibitors of Ca2+-ATPases, we now show the presence of SPCA and Ca2+ sto...

journal_title：FEBS letters

authors： Gunaratne HJ,Vacquier VD

更新日期：2006-07-10 00:00:00

abstract：:We have studied the modulation and pharmacological properties of two anion channels in T84 cells by recording single channel and transepithelial currents. One channel had an outwardly rectifying current-voltage I/V curve, was rarely active in cell-attached patches, and was unaffected by cAMP. The other channel had low...

journal_title：FEBS letters

authors： Tabcharani JA,Low W,Elie D,Hanrahan JW

更新日期：1990-09-17 00:00:00

abstract：:Interleukin-9 (IL-9) stimulation results in JAK, STAT and IRS1/2 phosphorylation. The role of IRS adaptor proteins in IL-9 signaling is not clear. We show that IL-9 induces IRS2 phosphorylation and association with phosphatidylinositol-3 kinase (PI 3-K) p85 subunit in TS1 cells and BaF/9R cells, which proliferate upon...

journal_title：FEBS letters

authors： Demoulin JB,Grasso L,Atkins JM,Stevens M,Louahed J,Levitt RC,Nicolaides NC,Renauld JC

更新日期：2000-10-06 00:00:00

abstract：:Plasma citrate levels were found to be elevated in non-alcoholic fatty liver disease (NAFLD) patients. Cellular experiments indicated that increased citrate levels might originate from an excess of fatty acids. The impact of elevated citrate levels on oxidative stress was examined. It was found that citrate stimulated...

journal_title：FEBS letters

authors： van de Wier B,Balk JM,Haenen GR,Giamouridis D,Bakker JA,Bast BC,den Hartog GJ,Koek GH,Bast A

更新日期：2013-08-02 00:00:00

abstract：:C1qTNF-related proteins (CTRPs) are involved in diverse processes including metabolism, inflammation host defense, apoptosis, cell differentiation, autoimmunity, hibernation, and organogenesis. However, the physiological role of CTRP6 remains poorly understood. Here we demonstrate that the globular domain of CTRP6 med...

journal_title：FEBS letters

authors： Lee W,Kim MJ,Park EJ,Choi YJ,Park SY

更新日期：2010-03-05 00:00:00

abstract：:The ABCG2 protein has a key role in the transport of a wide range of structurally dissimilar endo- and xenobiotics in the human body, especially in the tissue barriers and the metabolizing or secreting organs. The human ABCG2 gene harbors a high number of polymorphisms and mutations, which may significantly modulate i...

journal_title：FEBS letters

authors： Sarkadi B,Homolya L,Hegedűs T

更新日期：2020-10-04 00:00:00

abstract：:The inhibitory effects of various nitrogen-containing sugars on porcine kidney trehalase were studied. Validamycin A, validoxylamine A and MDL 25,637 were found to be potent, time-dependent inhibitors of the enzyme in vitro. The validoxylamine A-inhibited enzyme showed slow but reversible reactivation over time (t1/2 ...

journal_title：FEBS letters

authors： Salleh HM,Honek JF

更新日期：1990-03-26 00:00:00

abstract：:Glycine amide (GlyAd), a typically amidated amino acid, is a versatile additive that suppresses protein aggregation during refolding, heat treatment, and crystallization. In spite of its effectiveness, the exact mechanism by which GlyAd suppresses protein aggregation remains to be elucidated. Here, we show the crystal...

journal_title：FEBS letters

authors： Ito L,Shiraki K,Makino M,Hasegawa K,Kumasaka T

更新日期：2011-02-04 00:00:00

abstract：:The cytochrome bc1 complex is a key component of the mitochondrial respiratory chains of many eukaryotic microorganisms that are pathogenic for plants or humans, such as fungi responsible for crop diseases and Plasmodium falciparum, which causes human malaria. Cytochrome bc1 is an enzyme that contains two (ubi)quinone...

journal_title：FEBS letters

authors： Fisher N,Meunier B,Biagini GA

更新日期：2020-09-01 00:00:00

abstract：:CDK11(p58), a 58kDa protein of the PITSLRE kinase family, plays an important role in cell cycle progression, and is closely related to cell apoptosis. To gain further insight into the function of CDK11(p58), we screened a human fetal liver cDNA library for its interacting proteins using the yeast two-hybrid system. He...

journal_title：FEBS letters

authors： Zong H,Li Z,Liu L,Hong Y,Yun X,Jiang J,Chi Y,Wang H,Shen X,Hu Y,Niu Z,Gu J

更新日期：2005-07-04 00:00:00

abstract：:It was found for RNases of different specificities that changes in the free energy for substrate-enzyme binding induced by variations in the nucleotide base structure are accompanied by proportional changes in kcat/Km. This was considered to be a consequence of the strain in the enzyme-substrate complex. ...

journal_title：FEBS letters

authors： Yakovlev GI,Bocharov AL,Moiseyev GP

更新日期：1984-10-01 00:00:00

abstract：:A human Cu,Zn-superoxide dismutase (Cu,Zn-SOD) gene was fused with a gene fragment encoding the nine amino acid transactivator of transcription (Tat) protein transduction domain (RKKRRQRRR) of HIV-1 in a bacterial expression vector to produce a genetic in-frame Tat-SOD fusion protein. The expressed and purified Tat-SO...

journal_title：FEBS letters

authors： Kwon HY,Eum WS,Jang HW,Kang JH,Ryu J,Ryong Lee B,Jin LH,Park J,Choi SY

更新日期：2000-11-24 00:00:00

abstract：:The alpha3/beta4 subtype of neuronal nicotinic acetylcholine receptor (nAChR) was stably expressed in human embryonic kidney (HEK) 293 cells that co-expressed a voltage-gated Ca2+ channel. alpha3/beta4-nAChR-expressing clones were identified using the fura-2 Ca2+ imaging technique, and were further characterised by si...

journal_title：FEBS letters

authors： Stetzer E,Ebbinghaus U,Storch A,Poteur L,Schrattenholz A,Kramer G,Methfessel C,Maelicke A

更新日期：1996-11-11 00:00:00

abstract：:Recently, it has been shown that really interesting new gene (RING)-in between ring finger (IBR)-RING domain-containing proteins, such as Parkin and Parc, are E3 ubiquitin ligases and are involved in regulation of apoptosis. In this report, we show that p53-inducible RING-finger protein (p53RFP), a p53-inducible E3 ub...

journal_title：FEBS letters

authors： Huang J,Xu LG,Liu T,Zhai Z,Shu HB

更新日期：2006-02-06 00:00:00

abstract：:p38, which has been suggested to be a scaffold protein for the assembly of a macromolecular tRNA synthetase complex, contains a leucine zipper-like motif. To understand the importance of the leucine zipper-like motif of p38 (p38LZ) in macromolecular assembly, the p38LZ solution structure was investigated by circular d...

journal_title：FEBS letters

authors： Ahn HC,Kim S,Lee BJ

更新日期：2003-05-08 00:00:00

abstract：:The involvement of H2O2 generated by photosystem II (PSII) in the process of photoinhibition of thylakoids with a functional oxygen-evolving complex (OEC) was investigated. The rate of photoinhibition was decreased to the rate of loss of activity in the dark when bovine Fe-catalase was present during the photoinhibito...

journal_title：FEBS letters

authors： Bradley RL,Long KM,Frasch WD

更新日期：1991-07-29 00:00:00

abstract：:The further biochemical characterization and subcellular localization of cardiotin, a high molecular weight (300 kDa) constituent of cardiac muscle, is described. Immunofluorescence assays revealed a colocalization of cardiotin and the Ca2+ pump SERCA2a in the longitudinal sarcoplasmic reticulum (SR). However, in cont...

journal_title：FEBS letters

authors： Schaart G,Moens L,Endert JM,Ramaekers FC

更新日期：1997-02-17 00:00:00

abstract：:The effect of dexamethasone on mRNA and protein synthesis of lipocortins (LCT) 1, 2 and 5 has been investigated in U-937 cells. A constitutive expression of both mRNAs and proteins was detected in undifferentiated U-937 cells. This constitutive level was increased time- and dose-dependently by incubation with phorbol ...

journal_title：FEBS letters

authors： Solito E,Raugei G,Melli M,Parente L

更新日期：1991-10-21 00:00:00

abstract：:The thioredoxin-catalyzed insulin reduction by dihydrolipoate was applied to study the 2-oxoacid: lipoate oxidoreductase activity of 2-oxoacid dehydrogenase complexes. The enzymatic and non-enzymatic mechanisms of the transfer of reducing equivalents from the complexes to free lipoic acid (alpha-lipoic acid, 6,8-thioo...

journal_title：FEBS letters

authors： Bunik V,Shoubnikova A,Loeffelhardt S,Bisswanger H,Borbe HO,Follmann H

更新日期：1995-09-04 00:00:00

abstract：:The hexose transporter family of Saccharomyces cerevisiae comprises 18 proteins (Hxt1-17, Gal2). Here, we demonstrate that all these proteins, except Hxt12, and additionally three members of the maltose transporter family (Agt1, Ydl247, Yjr160) are able to transport hexoses. In a yeast strain deleted for HXT1-17, GAL2...

journal_title：FEBS letters

authors： Wieczorke R,Krampe S,Weierstall T,Freidel K,Hollenberg CP,Boles E

更新日期：1999-12-31 00:00:00