Abstract:
:A novel allergenic member of the family of Ca(2+)-binding proteins has been cloned from olive tree pollen. The isolated DNA codes for a protein of 171 amino acid residues, which displays four EF-hand sequence motifs. The encoded protein was overproduced in Escherichia coli and purified. The protein (18¿ omitted¿795 Da), which binds Ca(2+) and IgE antibodies from patients allergic to olive pollen, undergoes Ca(2+)-dependent conformational changes. It is retained on a phenyl-Sepharose column, which indicates the existence of regulatory EF-hand domains. This fact suggests its involvement in Ca(2+)-dependent signal transduction events of the pollen grain. This allergen could be considered as a member of a new subfamily of EF-hand Ca(2+)-binding proteins since it displays a low amino acid sequence similarity with the so far known proteins.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ledesma A,Villalba M,Rodríguez Rdoi
10.1016/s0014-5793(99)01790-1keywords:
subject
Has Abstractpub_date
2000-01-21 00:00:00pages
192-6issue
1eissn
0014-5793issn
1873-3468pii
S0014-5793(99)01790-1journal_volume
466pub_type
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