Abstract:
:We have studied the interaction of the yeast RAS2 protein with guanine nucleotides using energetic parameters for the dissociation of RAS.nucleotide complexes. The results indicated that a Gly-->Ser substitution at position 82 led to an altered interaction with GppNHp and, to a lesser extent, also with GDP. It was also possible to conclude that structural perturbation of Gly82 can stimulate nucleotide release by decreasing the energetic barrier for nucleotide dissociation. This, together with the observation that residues 80 and 81 are involved in the response of RAS to nucleotide exchange factors without affecting GDP binding per se, suggests a potential mechanism for exchange factor-stimulated GDP release.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
De Vendittis E,Fasano Odoi
10.1016/0014-5793(94)00521-4subject
Has Abstractpub_date
1994-06-27 00:00:00pages
133-6issue
2-3eissn
0014-5793issn
1873-3468pii
0014-5793(94)00521-4journal_volume
347pub_type
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