Identification of a unique splicing regulatory cluster in hepatitis B virus pregenomic RNA.

Abstract:

:HBV particles with genome derived from spliced mRNAs accumulate in patients with virus-derived severe liver necrosis and fibrosis. We investigated the role of an intronic element (intronic splicing silencer-long, ISS(L)) on splicing of HBV minigene transcripts. Removal of the entire ISS(L) showed two-fold increase in splicing while shorter deletions within ISS(L) indicated isolated clusters of activator and repressor domains. Activator domains stimulated splicing in presence of PRE, a long HBV 3' exon and even when present in a heterologous context. Mutations in the repressor domain unexpectedly augmented repression. The role of this intronic splicing regulatory element could be important for HBV pathogenesis.

journal_name

FEBS Lett

journal_title

FEBS letters

authors

Chowdhury JB,Roy D,Ghosh S

doi

10.1016/j.febslet.2011.09.026

subject

Has Abstract

pub_date

2011-10-20 00:00:00

pages

3348-53

issue

20

eissn

0014-5793

issn

1873-3468

pii

S0014-5793(11)00705-8

journal_volume

585

pub_type

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