Abstract:
:Accurate cDNA data is useful to validate gene structures in a genome. We sequenced 35189 expressed sequence tags (ESTs) obtained from the highly destructive rice blast fungus, Magnaporthe grisea. Our custom-made computational programs mapped these ESTs on the M. grisea genome sequence, and reconstructed gene structures as well as protein-coding regions. As a result, we predicted 4480 protein-coding sequences, which were more accurate than ab initio predictions. Moreover, cross-species comparisons suggested that our predicted proteins were nearly complete. The cDNA clones obtained in this study will be important for further experimental studies. Our genome annotation is available at http://www.mg.dna.affrc.go.jp/.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Numa H,Nishimura M,Tanaka T,Kanamori H,Yang CC,Matsumoto T,Nagamura Y,Itoh Tdoi
10.1016/j.febslet.2009.01.041subject
Has Abstractpub_date
2009-02-18 00:00:00pages
797-800issue
4eissn
0014-5793issn
1873-3468pii
S0014-5793(09)00075-1journal_volume
583pub_type
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