The effect of glycosyl inositol-phosphate on cAMP production in isolated rat fat-cells is transduced by a pertussis toxin sensitive G-protein.

abstract：:The Alanine Serine Cysteine Transporter 2 (ASCT2) is involved in balancing the intracellular amino acid pool. This function is allowed by the antiport mechanism and the asymmetric specificity towards different neutral amino acids, distinctive of this transporter. In the present work, the interaction of the putative su...

journal_title：FEBS letters

authors： Scalise M,Pochini L,Pingitore P,Hedfalk K,Indiveri C

更新日期：2015-11-30 00:00:00

abstract：:The present study was designed to investigate whether and how the purinergic stimulation of rat ventricular myocytes modulates the cAMP-dependent pathway. Stimulation of cardiomyocytes with ATPgammaS in the presence of the phosphodiesterase inhibitor IBMX increases by 3-fold intracellular cAMP content. In contrast to ...

journal_title：FEBS letters

authors： Pucéat M,Bony C,Jaconi M,Vassort G

更新日期：1998-07-17 00:00:00

abstract：:Synaptotagmin I, the calcium sensor for neurotransmission, is palmitoylated. We have identified the palmitoylation sites as five cysteine residues located between the transmembrane and cytoplasmic regions. In contrast to wild-type synaptotagmin, the non-acylated mutant is not converted to the endoglycosidase-H-resista...

journal_title：FEBS letters

authors： Heindel U,Schmidt MF,Veit M

更新日期：2003-06-05 00:00:00

abstract：:Only recently have products of the enzymatic breakdown of the chlorophylls been characterized for the first time. All catabolites isolated until now from a chlorophyte and from angiosperms have in common the position at which the primary oxygenative ring cleavage occurs, yielding linear tetrapyrroles (19-formyl-1[21H,...

journal_title：FEBS letters

authors： Curty C,Engel N,Gossauer A

更新日期：1995-05-01 00:00:00

abstract：:4-Picoloine-2,2':6',2"-terpyridine-platinum(II) is shown in a ligation assay to unwind and so intercalate into DNA. Circular dichroism is used to determine an equilibrium binding constant of approximately 2 x 10(7) M(-1) for the most stable binding mode of 4-picoline-2,2':6',2"-terpyridine-platinum(II) to poly[d(A-T)2...

journal_title：FEBS letters

authors： McCoubrey A,Latham HC,Cook PR,Rodger A,Lowe G

更新日期：1996-02-12 00:00:00

abstract：:The addition of 1-acyl-sn-glycero-3-phosphocholine (1-acyl-GPC) to peroxisomes decreased the production of acid-soluble radioactivity formed by beta-oxidation of [1-(14)C]arachidonate due to substrate removal by esterification into the acceptor. This peroxisomal-associated acyl-CoA:1-acyl-GPC acyltransferase activity ...

journal_title：FEBS letters

authors： Baykousheva SP,Luthria DL,Sprecher H

更新日期：1995-06-26 00:00:00

abstract：:A number of human diseases can be linked to aberrations in protein folding which cause an imbalance in protein homeostasis. Molecular chaperones, including heat shock proteins, act to assist protein folding, stability and activity in the cell. Attention has begun to focus on modulating the expression and/or activity o...

journal_title：FEBS letters

authors： Powers MV,Workman P

更新日期：2007-07-31 00:00:00

abstract：:The role of necrotic and apoptotic pathways in cytolysis mediated by LAK cells was studied. The contribution of necrotic and apoptotic processes to cytolysis depends both on the LAK cells' phenotype and the type of target cells. CD16+/CD8+/CD3- LAK cells induced necrosis of K562 and L929 target cells. The cell death i...

journal_title：FEBS letters

authors： Sashchenko LP,Kabanova OD,Blishchenko LYu,Lukjianova TI,Satpaev DK,Chertov OYu,Gnuchev NV

更新日期：1993-12-06 00:00:00

abstract：:Redox homeostasis is a prerequisite for survival of the pathogen Mycobacterium tuberculosis (Mtb) which employs the low molecular weight thiol mycothiol (MSH). The Mycobacterial NADPH-dependent mycothione reductase (MtMtr), composed of an NADPH-, FAD-, and a dimerization-domain connected by linkers, regulates the bala...

journal_title：FEBS letters

authors： Kumar A,Subramanian Manimekalai MS,Grüber G

更新日期：2018-02-01 00:00:00

abstract：:We show that inverted membrane vesicles from Corynebacterium glutamicum, a Gram-positive bacterium, are able to generate and maintain an electrochemical gradient of protons in response to the addition of NADH. This result indicates that the respiratory chain is intact and that the vesicles are reasonably impermeable t...

journal_title：FEBS letters

authors： Schrempp S,Bayan N,Shechter E

更新日期：1994-12-12 00:00:00

abstract：:In yeast, three AAA superfamily metalloproteases (Yme1p, Afg3p and Rca1p) are localized to the mitochondrial inner membrane where they perform roles in the assembly and turnover of the respiratory chain complexes. We have investigated the function of the proposed human orthologue of yeast Yme1p, encoded by the YME1L g...

journal_title：FEBS letters

authors： Shah ZH,Hakkaart GA,Arku B,de Jong L,van der Spek H,Grivell LA,Jacobs HT

更新日期：2000-08-04 00:00:00

abstract：:The F(1)F(0) ATP synthase has been purified from the hyperthermophilic eubacterium Aquifex aeolicus and characterized. Its subunits have been identified by MALDI-mass spectrometry through peptide mass fingerprinting and MS/MS. It contains the canonical subunits alpha, beta, gamma, delta and epsilon of F(1) and subunit...

journal_title：FEBS letters

authors： Peng G,Bostina M,Radermacher M,Rais I,Karas M,Michel H

更新日期：2006-10-30 00:00:00

abstract：:Heme is essential and synthesized via highly regulated processes. For this reason, most organisms strive to recycle it or acquire it from their environment. When heme is bound to proteins noncovalently, degradation of the polypeptide is sufficient to release it. However, in some hemoproteins, such as c-type cytochrome...

journal_title：FEBS letters

authors： Murphey AC,Mavridou DAI,Hodgkin J,Ferguson SJ

更新日期：2018-03-01 00:00:00

abstract：:Data obtained from peptide mapping of the active and inactive forms of human renin show that there are extensive regions of common sequence in the two forms of the enzyme, and are consistent with the hypothesis that inactive renin is a renin zymogen. ...

journal_title：FEBS letters

authors： McIntyre GD,Chaudhuri S,Coggins JR

更新日期：1984-10-01 00:00:00

abstract：:The NAD+-requiring enzymes of glycoside hydrolase family 4 (GHF4) contain a region with a conserved Gly-XXX-Gly-Ser (GXGS) motif near their N-termini that is reminiscent of the fingerprint region of the Rossmann fold, a conserved structural motif of classical nicotinamide nucleotide-binding proteins. The function of t...

journal_title：FEBS letters

authors： Raasch C,Armbrecht M,Streit W,Höcker B,Sträter N,Liebl W

更新日期：2002-04-24 00:00:00

abstract：:We compared oxidative DNA damage in strictly anaerobic Prevotella melaninogenica, aerotolerant anaerobic Bacteroides fragilis, and facultative anaerobic Salmonella typhimurium after exposure to O2 or H2O2. Using HPLC with electrochemical detection, we measured 8-hydroxydeoxyguanosine (8OHdG) as a damage marker. O2 ind...

journal_title：FEBS letters

authors： Takeuchi T,Nakaya Y,Kato N,Watanabe K,Morimoto K

更新日期：1999-05-07 00:00:00

abstract：:A novel allergenic member of the family of Ca(2+)-binding proteins has been cloned from olive tree pollen. The isolated DNA codes for a protein of 171 amino acid residues, which displays four EF-hand sequence motifs. The encoded protein was overproduced in Escherichia coli and purified. The protein (18¿ omitted¿795 Da...

journal_title：FEBS letters

authors： Ledesma A,Villalba M,Rodríguez R

更新日期：2000-01-21 00:00:00

abstract：:Ischemia/reperfusion induces nuclear factor kappaB (NF-kappaB) and AP-1 in rat hearts after 15 min of ischemia followed by reperfusion (R) for various periods of time (15 and 30 min, 1, 2, 3, 6, 12, and 24 h). Low levels of NF-kappaB and no signal for AP-1 were detected in shams and in non-ischemic tissue distant from...

journal_title：FEBS letters

authors： Chandrasekar B,Freeman GL

更新日期：1997-01-13 00:00:00

abstract：:Streptomyces lividans 1326-9, a bldA+ strain, and its bldA39 mutant derivative J1725 were transformed with a cosmid containing the pur cluster, which determines the puromycin biosynthetic pathway from Streptomyces alboniger. bldA+ transformants produced puromycin in typical amounts, whereas bldA39 transformants did so...

journal_title：FEBS letters

authors： Tercero JA,Espinosa JC,Jiménez A

更新日期：1998-01-16 00:00:00

abstract：:In plant organelle genomes, homeologous recombination between heteroallelic positions of repetitive sequences is increased by dysfunction of the gene encoding MutS homolog 1 (MSH1), a plant organelle-specific homolog of bacterial mismatch-binding protein MutS1. The C-terminal region of plant MSH1 contains the GIY-YIG ...

journal_title：FEBS letters

authors： Fukui K,Harada A,Wakamatsu T,Minobe A,Ohshita K,Ashiuchi M,Yano T

更新日期：2018-12-01 00:00:00

abstract：:Cyanobacteria possess genes encoding phytochrome-related proteins. We used a DNA microarray approach to evaluate the impact of the phytochromes Cph1 and Cph2 on red light (R)- and far-red light (FR)-dependent gene expression in the unicellular cyanobacterium Synechocystis sp. PCC 6803. In cells of wild-type and phytoc...

journal_title：FEBS letters

authors： Hübschmann T,Yamamoto H,Gieler T,Murata N,Börner T

更新日期：2005-03-14 00:00:00

abstract：:The rate of whole cell H2O2 metabolism in several salivarian and stercorarian trypanosomes and Leishmania species was measured. These cells metabolized H2O2 at rates between 2.3 and 48.2 nmol/10(8) cells per min depending upon the species employed. H2O2 metabolism was largely insensitive to NaN3, implying that typical...

journal_title：FEBS letters

authors： Penketh PG,Kennedy WP,Patton CL,Sartorelli AC

更新日期：1987-09-14 00:00:00

abstract：:In exocrine acinar cells a variety of neurotransmitters (e.g. acetylcholine) stimulate phosphatidylinositol 4,5-bisphosphate hydrolysis elevating intracellular calcium to activate calcium-dependent membrane currents (outward K+ and inward Cl-). This study shows that in lacrimal acinar cells extracellular application o...

journal_title：FEBS letters

authors： Sasaki T,Gallacher DV

更新日期：1990-05-07 00:00:00

abstract：:The temperature dependence of single-channel conductance and open probability for outer membrane protein A (OmpA) of Escherichia coli were examined in planar lipid bilayers. OmpA formed two interconvertible conductance states, small channels, 36-140 pS, between 15 and 37 degrees C, and large channels, 115-373 pS, betw...

journal_title：FEBS letters

authors： Zakharian E,Reusch RN

更新日期：2003-12-04 00:00:00

abstract：:According to the leading theory, the agent responsible for prion diseases would be the conformational isomer PrP(Sc) of a cellular protein PrP(C), the pathogenic form PrP(Sc) multiplying by converting the normal protein into a likeness of itself. The pathogenic isoform could catalyze the conformational transition so t...

journal_title：FEBS letters

authors： Laurent M

更新日期：1997-04-21 00:00:00

abstract：:We investigated the signaling pathways participating in hyperosmotic regulation of the human taurine transporter (TAUT) by using specific inhibitors of various intracellular signaling molecules. Among them, the specific inhibitor of calcium/calmodulin-dependent protein kinase II (Ca(2+)/CaM kinase II) completely repre...

journal_title：FEBS letters

authors： Satsu H,Manabe M,Shimizu M

更新日期：2004-07-02 00:00:00

abstract：:A one-electron reductive metabolism of 1,2-dibromoethane (DBE) is described that gives rise to a free radical intermediate, which can be stabilized by a spin trapping agent and detected by electron spin resonance spectroscopy. Using rat liver microsomes or isolated hepatocytes from phenobarbitone pretreated animals, u...

journal_title：FEBS letters

authors： Tomasi A,Albano E,Dianzani MU,Slater TF,Vannini V

更新日期：1983-08-22 00:00:00

abstract：:Human CBS1 is a methionine sulfoxide reductase of type B (MSRB) as it specifically reduced Met-R-SO in peptides with dithiothreitol or the thioredoxin system as reductants. Mutation C169S in the active site completely abolished enzymatic activity, while mutation W110A only reduced activity and C105S had no effect. Lik...

journal_title：FEBS letters

authors： Jung S,Hansel A,Kasperczyk H,Hoshi T,Heinemann SH

更新日期：2002-09-11 00:00:00

abstract：:We have constructed cDNA clones covering the entire coding region of mouse, human and rabbit preprocathepsin K mRNA for studies on bone turnover. The clone pMCatK-1 for mouse cathepsin K shares 87% nucleotide homology with the corresponding human and rabbit sequences. Analysis of a panel of mouse tissues for tissue di...

journal_title：FEBS letters

authors： Rantakokko J,Aro HT,Savontaus M,Vuorio E

更新日期：1996-09-16 00:00:00

abstract：:Studies of mutants of the yeast Saccharomyces cerevisiae have led to the identification of genes required for high affinity iron uptake. Reduction of iron (III) outside the cell is accomplished by means of reductases encoded by FRE1 and FRE2, homologues of the gp91-phox component of the oxygen reductase of human granu...

journal_title：FEBS letters

authors： Klausner RD,Dancis A

更新日期：1994-11-28 00:00:00