Ectodomain shedding of the EGF-receptor ligand epigen is mediated by ADAM17.


:All ligands of the epidermal growth factor receptor (EGFR), which has important roles in development and disease, are made as transmembrane precursors. Proteolytic processing by ADAMs (a disintegrin and metalloprotease) regulates the bioavailability of several EGFR-ligands, yet little is known about the enzyme responsible for processing the recently identified EGFR ligand, epigen. Here we show that ectodomain shedding of epigen requires ADAM17, which can be stimulated by phorbol esters, phosphatase inhibitors and calcium influx. These results suggest that ADAM17 might be a good target to block the release of bioactive epigen, a highly mitogenic ligand of the EGFR which has been implicated in wound healing and cancer.




FEBS letters


Sahin U,Blobel CP




Has Abstract


2007-01-09 00:00:00














  • A recombinant polypeptide model of the second predicted nucleotide binding fold of the cystic fibrosis transmembrane conductance regulator is a GTP-binding protein.

    abstract::Association reactions of a recombinant CFTR-NBF-2 polypeptide fused to glutathione S-transferase with guanine nucleotides were monitored quantitatively by recording the fluorescence enhancement of excited trinitrophenol (TNP)-labelled GTP after binding to NBF-2. Binding of TNP-GTP to the recombinant NBF-2 polypeptide ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Randak C,Neth P,Auerswald EA,Assfalg-Machleidt I,Roscher AA,Hadorn HB,Machleidt W

    更新日期:1996-11-25 00:00:00

  • Two antifungal thaumatin-like proteins from barley grain.

    abstract::Antifungal activity has been associated with 2 immunochemically distinct proteins, protein R and S (Mr approximately 23 kDa; pI 9-10), which were isolated in pure form from barley grain. The proteins are homologous with thaumatin- and pathogenesis-related proteins of the PR5 family. The proteins inhibit growth of i.a....

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hejgaard J,Jacobsen S,Svendsen I

    更新日期:1991-10-07 00:00:00

  • Na,K-ATPase labelled with 5-iodoacetamidofluorescein: E2-E1 conformational transition induced by different nucleotides.

    abstract::A conformational transition between E2 and E1 forms of Na, K-ATPase induced by different nucleotides has been studied under steady state conditions using the enzyme labelled with 5-iodoacetamidofluorescein. In the presence of K+ the plot of fluorescence as a function of [ATP], [ADP] or [CTP] (in a range of 5 microM-12...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Lopina OD,Pindel EV,Boldyrev AA

    更新日期:1990-06-18 00:00:00

  • Evaluation of metal-conjugated compounds as inhibitors of 3CL protease of SARS-CoV.

    abstract::3C-like (3CL) protease is essential for the life cycle of severe acute respiratory syndrome-coronavirus (SARS-CoV) and therefore represents a key anti-viral target. A compound library consisting of 960 commercially available drugs and biologically active substances was screened for inhibition of SARS-CoV 3CL protease....

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Hsu JT,Kuo CJ,Hsieh HP,Wang YC,Huang KK,Lin CP,Huang PF,Chen X,Liang PH

    更新日期:2004-09-10 00:00:00

  • Cytokinin affinity purification and identification of a tobacco BY-2 adenosine kinase.

    abstract::Adenosine kinase is one of the enzymes potentially responsible for the formation of cytokinin nucleotides in plants. Using a zeatin affinity column a 40 kDa protein was isolated from tobacco Bright Yellow 2 (TBY-2) and identified by mass spectrometry as adenosine kinase. The ligand interaction reported here can be dis...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Laukens K,Lenobel R,Strnad M,Van Onckelen H,Witters E

    更新日期:2003-01-02 00:00:00

  • Phosphorylation of high- and low-molecular-mass atrial natriuretic peptide analogs by cyclic AMP-dependent protein kinase.

    abstract::Synthetic high- and low-molecular-mass atrial peptides were phosphorylated in vitro by cyclic AMP-dependent protein kinase and [32P]ATP. From a series of atrial peptide analogs, it was deduced that the amino acid sequence, Arg101-Ser104 of atriopeptin was required for optimal phosphorylation. Phosphorylated AP(99-126)...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Olins GM,Mehta PP,Blehm DJ,Patton DR,Zupec ME,Whipple DE,Tjoeng FS,Adams SP,Olins PO,Gierse JK

    更新日期:1987-11-30 00:00:00

  • Antithrombin Budapest 3. An antithrombin variant with reduced heparin affinity resulting from the substitution L99F.

    abstract::The molecular basis and functional properties of a variant antithrombin (AT) protein. AT Budapest 3, were studied. A single base substitution was identified in codon 99, CTC----TTC, altering the normal leucine to phenylalanine. The proband presented with a history of venous thrombotic disease and was found to be homoz...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Olds RJ,Lane DA,Boisclair M,Sas G,Bock SC,Thein SL

    更新日期:1992-04-06 00:00:00

  • ADP-ribosylation of nuclear proteins is increased by phenobarbital. Identification of the ADP-ribosylated histone fractions in rat liver nuclei.

    abstract::Changes in the ADP-ribosylation of total proteins and purified histones of rat liver nuclei after phenobarbital treatment (80 mg/kg, 24 h) have been studied. The [32P]NAD incorporation into total trichloroacetic acid precipitated proteins, in histone Hl and in core histones was evaluated, the specific radioactivities ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Bràz J,Lechner MC

    更新日期:1986-04-21 00:00:00

  • Influence of neopterin on the generation of reactive oxygen species in human neutrophils.

    abstract::Neopterin is synthesized by human monocyte-derived macrophages primarily upon stimulation with the cytokine interferon-gamma. We studied the influence of neopterin on the generation of reactive oxygen species (ROS) in human peripheral blood neutrophils. Radical formation was measured using a biochemiluminometer. Neutr...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Razumovitch JA,Semenkova GN,Fuchs D,Cherenkevich SN

    更新日期:2003-08-14 00:00:00

  • Cross-linking of a synthetic partial-length (1-28) peptide of the Alzheimer beta/A4 amyloid protein by transglutaminase.

    abstract::Cerebral deposits of beta/A4 amyloid protein is a pathologic sign of Alzheimer's disease. A synthetic partial-length (1-28) peptide of this protein contains one glutamine and two lysine residues. Here we show that this peptide can be a substrate of transglutaminase, which catalyzes cross-linking between glutamine and ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ikura K,Takahata K,Sasaki R

    更新日期:1993-07-12 00:00:00

  • A lipid transfer protein binds to a receptor involved in the control of plant defence responses.

    abstract::Lipid transfer proteins (LTPs) and elicitins are both able to load and transfer lipidic molecules and share some structural and functional properties. While elicitins are known as elicitors of plant defence mechanisms, the biological function of LTP is still an enigma. We show that a wheat LTP1 binds with high affinit...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Buhot N,Douliez JP,Jacquemard A,Marion D,Tran V,Maume BF,Milat ML,Ponchet M,Mikès V,Kader JC,Blein JP

    更新日期:2001-11-30 00:00:00

  • Structural features in aminoacyl-tRNAs required for recognition by elongation factor Tu.

    abstract::In bacterial polypeptide synthesis aminoacyl-tRNA (aa-tRNA) bound to elongation factor Tu (EF-Tu) and GTP is part of a crucial intermediate ribonucleoprotein complex involved in the decoding of messenger RNA. The conformation and topology as well as the affinity of the macromolecules in this ternary aa-tRNA X EF-Tu X ...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Faulhammer HG,Joshi RL

    更新日期:1987-06-15 00:00:00

  • Dictyostelium discoideum requires an Alix/AIP1 homolog, DdAlix, for morphogenesis in alkaline environments.

    abstract::Alix and its homologs are involved in various phenomena such as endosomal protein-sorting and adaptation to stress conditions. In this study, we found that development of Dictyostelium discoideum Alix (DdAlix) deletion mutant (alx-) cells was impaired in alkaline pH environments. The fruiting body formation efficiency...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Ohkouchi S,Saito H,Aruga F,Maeda T,Shibata H,Maki M

    更新日期:2005-03-14 00:00:00

  • Effect of rutin and its copper complex on superoxide formation and lipid peroxidation in rat liver microsomes.

    abstract::Two free radical scavengers, bioflavonoid rutin and the copper-rutin complex Cu(Rut)Cl2, inhibited lucigenin-amplified chemiluminescence and lipid peroxidation in rat liver microsomes, Cu(Rut)Cl2 being a 5-9 times more efficient inhibitor than rutin. The enhanced inhibitory activity of Cu(Rut)Cl2 was due to the presen...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Afanas'ev IB,Ostrachovich EA,Korkina LG

    更新日期:1998-03-27 00:00:00

  • Biogenesis of Y RNA-derived small RNAs is independent of the microRNA pathway.

    abstract::Y RNAs are approximately 100 nucleotide long conserved cytoplasmic non-coding RNAs, which produce smaller RNA fragments during apoptosis. Here we show that these smaller RNA molecules are also produced in non-stressed cells and in a range of human cancerous and non-cancerous cell types. Recent reports have speculated ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nicolas FE,Hall AE,Csorba T,Turnbull C,Dalmay T

    更新日期:2012-04-24 00:00:00

  • Inactivation of spermidine N1-acetyltransferase with alkaline phosphatase.

    abstract::Spermidine N1-acetyltransferase in an extract from phytohemagglutinin-stimulated bovine lymphocytes was inactivated by preincubation with alkaline phosphatase. Inactivation of the acetylase with the phosphatase was totally inhibited by addition of pyrophosphate. These results suggest that spermidine N1-acetyltransfera...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Matsui I,Otani S,Kamei M,Morisawa S

    更新日期:1982-12-13 00:00:00

  • Molecular cloning of the cDNA coding for Xenopus laevis prion protein.

    abstract::Isolation and characterization of the cDNA coding for the 216-residue Xenopus laevis prion protein is reported. Existence of this protein in amphibians was suggested by an EST fragment (accession number BG813008), while a conclusive demonstration is presented here. This protein exhibits a higher identity level to avia...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Strumbo B,Ronchi S,Bolis LC,Simonic T

    更新日期:2001-11-16 00:00:00

  • An inverse relationship between receptor internalization and the fraction of laterally mobile receptors for the vasopressin renal-type V2-receptor. An active role for receptor immobilization in down-regulation?

    abstract::Lateral mobility of the vasopressin renal-type V2-receptor was investigated in LLC-PK1 porcine epithelial cells using the technique of fluorescence microphotolysis (photobleaching) and a rhodamine-labelled vasopressin analogue. At various times after ligand addition, cells were analyzed for both receptor lateral mobil...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Jans DA,Peters R,Fahrenholz F

    更新日期:1990-11-12 00:00:00

  • Porcine tissue plasminogen activator. Immunoaffinity purification, structural properties and glycosylation pattern.

    abstract::Tissue plasminogen activator was purified in high yield from pig heart by immunoaffinity chromatography and characterized by analysis of the glycosylation pattern and the N-terminal amino acid sequence. Comparisons with the human enzyme reveals residue exchanges in the A-chain at positions 3 (porcine Arg/human Gln) an...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Pohl G,Jörnvall H,Kok P,Wallén P

    更新日期:1986-09-01 00:00:00

  • Water channel activities of Mimosa pudica plasma membrane intrinsic proteins are regulated by direct interaction and phosphorylation.

    abstract::cDNAs encoding aquaporins PIP1;1, PIP2;1, and TIP1;1 were isolated from Mimosa pudica (Mp) cDNA library. MpPIP1;1 exhibited no water channel activity; however, it facilitated the water channel activity of MpPIP2;1 in a phosphorylation-dependent manner. Mutagenesis analysis revealed that Ser-131 of MpPIP1;1 was phospho...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Temmei Y,Uchida S,Hoshino D,Kanzawa N,Kuwahara M,Sasaki S,Tsuchiya T

    更新日期:2005-08-15 00:00:00

  • Heterogeneous turnover of terminal and core sugars within the carbohydrate chain of dipeptidylaminopeptidase IV isolated from rat liver plasma membrane.

    abstract::Dipeptidylaminopeptidase IV, a plasma membrane-bound glycoprotein, is characterized by an intramolecular heterogeneous turnover of the protein backbone and carbohydrate chain. The faster turnover of the latter is restricted only to the outer sugars. The inner core sugars D-mannose and N-acetyl-D-glucosamine turn over ...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Volk BA,Kreisel W,Köttgen E,Gerok W,Reutter W

    更新日期:1983-10-31 00:00:00

  • Chemoenzymatic synthesis of 6-phospho-cyclophellitol as a novel probe of 6-phospho-β-glucosidases.

    abstract::Covalent, mechanism-based inhibitors of glycosidases are valuable probe molecules for visualizing enzyme activities in complex systems. We, here, describe the chemoenzymatic synthesis of 6-phospho-cyclophellitol and evaluate its behaviour as a mechanism-based inactivator of the Streptococcus pyogenes 6-phospho-β-gluco...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Kwan DH,Jin Y,Jiang J,Chen HM,Kötzler MP,Overkleeft HS,Davies GJ,Withers SG

    更新日期:2016-02-01 00:00:00

  • 2'-OH of mRNA are critical for the binding of its codons at the 40S ribosomal P site but not at the mRNA entry site.

    abstract::The roles of 2'-OH groups in the binding of mRNA to human ribosomes were studied using site-directed cross-linking. We found that both mRNA and mDNA analogues bearing a cross-linker can modify ribosomal proteins (rps) S3e and S2e at the mRNA entry site independently on tRNA presence, but only mRNA analogues were capab...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Graifer D,Zhigailov A,Ven'yaminova A,Malygin A,Iskakov B,Karpova G

    更新日期:2012-10-19 00:00:00

  • The impact of PEPC phosphorylation on growth and development of Arabidopsis thaliana: molecular and physiological characterization of PEPC kinase mutants.

    abstract::Two phosphoenolpyruvate carboxylase (PEPC) kinase genes (PPCk1 and PPCk2) are present in the Arabidopsis genome; only PPCk1 is expressed in rosette leaves. Homozygous lines of two independent PPCk1 T-DNA-insertional mutants showed very little (dln1), or no (csi8) light-induced PEPC phosphorylation and a clear retard i...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Meimoun P,Gousset-Dupont A,Lebouteiller B,Ambard-Bretteville F,Besin E,Lelarge C,Mauve C,Hodges M,Vidal J

    更新日期:2009-05-19 00:00:00

  • Lipopolysaccharide signaling induces serum amyloid A (SAA) synthesis in human hepatocytes in vitro.

    abstract::To investigate the role of lipopolysaccharide (LPS) in hepatocyte activation, we examined the expression of Toll-like receptor 4 (TLR4), the putative receptor for LPS in human hepatocytes. TLR4 mRNA and protein expression was confirmed in human hepatocytes. Stimulation of human hepatocytes with LPS results in rapid de...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Migita K,Abiru S,Nakamura M,Komori A,Yoshida Y,Yokoyama T,Daikoku M,Ueki T,Takii Y,Yano K,Yastuhashi H,Eguchi K,Ishibashi H

    更新日期:2004-07-02 00:00:00

  • Astrocyte differentiation mediated by LIF in cooperation with BMP2.

    abstract::Leukemia inhibitory factor (LIF) and bone morphogenetic protein (BMP) 2 signal via different receptor systems. We have recently demonstrated that simultaneous stimulation of fetal mouse neuroepithelial cells with these distinct types of cytokines synergistically induces astrocyte differentiation in a 2-day culture. He...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Nakashima K,Yanagisawa M,Arakawa H,Taga T

    更新日期:1999-08-20 00:00:00

  • Oligomeric structure of 14-3-3 protein: what do we know about monomers?

    abstract::14-3-3s predominantly form homo-/heterodimers that are in equilibrium with corresponding monomers. Dimer/monomer equilibrium depends on the nature and phosphorylation of Ser58 of certain 14-3-3 isoforms. The structure and properties of 14-3-3 dimers are well characterized, whereas 14-3-3 monomers are less investigated...

    journal_title:FEBS letters

    pub_type: 杂志文章,评审


    authors: Sluchanko NN,Gusev NB

    更新日期:2012-12-14 00:00:00

  • Stereospecific bioactions of 5-hydroxyeicosatetraenoate.

    abstract::5(S)-Hydroxyicosatetraenoate stimulates human polymorphonuclear neutrophils (PMNs) to raise their cytosolic calcium. It also potentiates the cells' degranulation responses to platelet-activating factor and diacylglycerols. We synthesized 5(R)-hydroxyicosatetraenoate and found it to be 20-100-fold weaker than the natur...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Rossi AG,Thomas MJ,O'Flaherty JT

    更新日期:1988-11-21 00:00:00

  • α-Synuclein modifies huntingtin aggregation in living cells.

    abstract::Several neurodegenerative disorders are characterized by the accumulation of proteinaceous inclusions in the central nervous system. These inclusions are frequently composed of a mixture of aggregation-prone proteins. Here, we used a bimolecular fluorescence complementation assay to study the initial steps of the co-a...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Herrera F,Outeiro TF

    更新日期:2012-01-02 00:00:00

  • Farnesyl-diphosphate farnesyltransferase 1 regulates hepatitis C virus propagation.

    abstract::To identify the novel genes involved in lipid metabolism and lipid droplet formation that may play important roles in Hepatitis C virus (HCV) propagation, we have screened the small interfering RNA library using cell culture derived HCV (HCVcc)-infected cells. We selected and characterized the gene encoding farnesyl-d...

    journal_title:FEBS letters

    pub_type: 杂志文章


    authors: Park EM,Nguyen LN,Lim YS,Hwang SB

    更新日期:2014-05-02 00:00:00