Regulation of the MAP kinase cascade in PC12 cells: B-Raf activates MEK-1 (MAP kinase or ERK kinase) and is inhibited by cAMP.

abstract：:DNA-dependent RNA polymerase from blood forms and culture forms of the parasitic protozoan Trypanosoma brucei was resolved into multiple peaks of activity by DEAE-Sephadex chromatography. The enzyme from the two forms was found to be different by several criteria, suggesting that it is subject to developmental control...

journal_title：FEBS letters

authors： Tittawella I

更新日期：1988-01-25 00:00:00

abstract：:The omega3 fatty-acid desaturases: FAD7 and FAD8 (plastid) and FAD3 (reticular) are responsible for trienoic fatty-acid (TA) production in plants. The expression of these enzymes seemed to be regulated differently in response to light. Darkness leads to a decrease in total TA level. Under such conditions, FAD3 and FAD...

journal_title：FEBS letters

authors： Collados R,Andreu V,Picorel R,Alfonso M

更新日期：2006-09-04 00:00:00

abstract：:We performed microarray and computational gene network analyses to identify the detailed mechanisms by which sodium butyrate (SB) induces cell growth arrest and the differentiation of mouse colonic epithelial MCE301 cells. Two thousand six hundred four differentially expressed probe sets were identified in the cells t...

journal_title：FEBS letters

authors： Tabuchi Y,Takasaki I,Doi T,Ishii Y,Sakai H,Kondo T

更新日期：2006-05-29 00:00:00

abstract：:Undifferentiated rat pheochromocytoma PC12 cells were voltage clamped using the whole cell technique. After blockade of outward currents, calcium currents were elicited from -40 and -100 mV. A subpopulation of cells displayed only one current component activated at -10 mV and slowly decaying. In other cells this curre...

journal_title：FEBS letters

authors： Janigro D,Maccaferri G,Meldolesi J

更新日期：1989-09-25 00:00:00

abstract：:The ATPase Cdc48 is required for membrane fusion and protein degradation. Recently it has been suggested that Cdc48 in a complex with Ufd1 and Npl4 acts as an ubiquitin-dependent chaperone. Here it is shown that recombinant Cdc48 alone can distinguish between the native and the non-native conformation of model substra...

journal_title：FEBS letters

authors： Thoms S

更新日期：2002-06-05 00:00:00

abstract：:The fluorescent Ca2+ indicator Fura 2 was used to monitor Ca2+ release induced by the Ins(1,4,5)P3-mobilizing agonist angiotensin II (Ag II), caffeine and 2,5-di-(tert-butyl)-1,4-benzohydroquinone (tuBHQ), in intact bovine adrenal chromaffin cells. Under low external Ca2+ conditions, tuBHQ, Ag II and caffeine elicited...

journal_title：FEBS letters

authors： Robinson IM,Burgoyne RD

更新日期：1991-09-09 00:00:00

abstract：:Bovine leukaemia virus (BLV) is the aetiological agent of Leukosis enzootica bovis [Viral Oncology (1980), G. Klein (Ed.) Raven Press, New York, pp. 231-238], a widely spread disease in cattle. BLV is reported as the animal model of human T-cell leukaemia virus (HLTV) which is the causative agent of adult T-cell leuka...

journal_title：FEBS letters

authors： Précigoux G,Geoffre S,Léonard R,Llido S,Dautant A,d'Estaintot BL,Picard P,Ménard A,Guillemain B,Hospital M

更新日期：1993-07-12 00:00:00

abstract：:Biotops with extreme temperatures such as deserts force animals to avoid or escape high temperatures by biochemical, behavioural or morphological adaptation. In this context we tested the resistance to heat of the oxygen carrier hemocyanin from the ancient tarantula Eurypelma californicum, which is found in arid zones...

journal_title：FEBS letters

authors： Sterner R,Vogl T,Hinz HJ,Penz F,Hoff R,Föll R,Decker H

更新日期：1995-05-01 00:00:00

abstract：:Oscillations of free intracellular calcium [Ca2+]i were seen in individual Jurkat cells as response to a 50 Hz, 0.15 mT magnetic field (MF). In contrast, a CD45-deficient Jurkat cell line was unable to respond to MF stimulation. The phosphatase activity of CD45 has been implicated to regulate p56lck tyrosine kinase ac...

journal_title：FEBS letters

authors： Lindström E,Berglund A,Mild KH,Lindström P,Lundgren E

更新日期：1995-08-14 00:00:00

abstract：:Cap-independent translation (CIT) occurs at the leader sequences of uncapped plant viral RNAs, but also at a number of normally capped cellular mRNAs and has been correlated with sequence complementarity to 18S rRNA. The ribosomal protein S18 (RPS18) is a component of the small ribosomal subunit and is encoded by thre...

journal_title：FEBS letters

authors： Vanderhaeghen R,De Clercq R,Karimi M,Van Montagu M,Hilson P,Van Lijsebettens M

更新日期：2006-05-15 00:00:00

abstract：:Analysis of glutamic acid decarboxylase (GDC) (EC 4.1.1.15) from Escherichia coli ATCC 11246 revealed the presence of six pyridoxal phosphates (PLPs) as well as six covalently bound pyrroloquinoline quinones (PQQs) per hexameric enzyme molecule. This is the second example of a pyridoxo-quinoprotein, suggesting that ot...

journal_title：FEBS letters

authors： van der Meer RA,Groen BW,Duine JA

更新日期：1989-03-27 00:00:00

abstract：:PCR-coupled cDNA subtraction hybridization was adapted to identify the genes expressed in the adrenocortical tissues from high salt diet-treated rat. A novel cDNA clone, termed salt-inducible kinase (SIK), encoding a polypeptide (776 amino acids) with significant similarity to protein serine/ threonine kinases in the ...

journal_title：FEBS letters

authors： Wang Z,Takemori H,Halder SK,Nonaka Y,Okamoto M

更新日期：1999-06-18 00:00:00

abstract：:A 1000-bp fragment of Enterococcus hirae genomic DNA was amplified by the polymerase chain reaction method, using the oligonucleotide primers designed from amino acid sequences of both amino-terminal and a tryptic fragment of the Na(+)-ATPase alpha-subunit in this organism. DNA sequencing of this product revealed that...

journal_title：FEBS letters

authors： Kakinuma Y,Igarashi K,Konishi K,Yamato I

更新日期：1991-11-04 00:00:00

abstract：:Hypohalous acids are poor one-electron oxidizing agents, such that reactions with hydrogen peroxide to yield radical species are not feasible. However, the oxidation of superoxide by hypohalous acids can be a source of hydroxyl or haline radicals. The oxidation of nitrogen monoxide by hypochlorous acid is favourable, ...

journal_title：FEBS letters

authors： Koppenol WH

更新日期：1994-06-20 00:00:00

abstract：:Here we make a brief survey of the present state of antibiotic research and use. We also describe a novel antibiotic that contains a basic peptide covalently bound to a morpholino oligonucleotide. ...

journal_title：FEBS letters

authors： Altman S

更新日期：2014-01-03 00:00:00

abstract：:The effects of insulin and IGF-I on the cell surface quantities of GLUT1, GLUT3 and GLUT4 glucose transporters in L6 myotubes were determined with the exofacial bis-mannose phololabel (ATB-BMPA). In basal cells, an equal molar quantity of each transporter isoform was found at the cell surface. Insulin stimulated the t...

journal_title：FEBS letters

authors： Wilson CM,Mitsumoto Y,Maher F,Klip A

更新日期：1995-07-10 00:00:00

abstract：:Recent studies have revealed that the sensory nervous system is involved in bone metabolism. However, the mechanism of communication between neurons and osteoblasts is yet to be elucidated. In this study, we investigated the signaling pathways between sensory neurons of the dorsal root ganglion (DRG) and the osteoblas...

journal_title：FEBS letters

authors： Kodama D,Hirai T,Kondo H,Hamamura K,Togari A

更新日期：2017-02-01 00:00:00

abstract：:The peroxisome assembly factor-1 (PAF-1) is reported here to contain the signature subsequence for a ring finger motif in its carboxyl-terminus. This conserved subsequence in PAF-1 may be the key to a gene expression regulatory pathway important in peroxisome biogenesis. ...

journal_title：FEBS letters

authors： Patarca R,Fletcher MA

更新日期：1992-11-02 00:00:00

abstract：:To test if mitochondrial uncoupling in white adipocytes is responsible for obesity resistance of the aP2-Ucp transgenic mice expressing ectopic uncoupling protein 1 (UCPI) in white fat, mitochondrial membrane potential (delta psi(m)) was estimated by flow cytometry in adipocytes isolated from gonadal fat. Ectopic UCP1...

journal_title：FEBS letters

authors： Baumruk F,Flachs P,Horáková M,Floryk D,Kopecký J

更新日期：1999-02-12 00:00:00

abstract：:Cultured human fibroblasts isolated from embryonic muscle, skin and peripheral nerve tissues were found to accumulate [3H]L-glutamate by a Na(+)-dependent uptake process strongly inhibited by several glutamate/aspartate analogues including D- and L-aspartate, D- and L-threo-3-hydroxyaspartate and L-trans-pyrrolidine-2...

journal_title：FEBS letters

authors： Balcar VJ,Shen J,Bao S,King NJ

更新日期：1994-02-14 00:00:00

abstract：:Monocyte chemoattractant protein-1 (MCP-1) is a member of the beta chemokine family which acts through specific seven transmembrane receptors to recruit monocytes, basophils, and T lymphocytes to sites of inflammation. To identify regions of the human MCP-1 protein which are important for its biological activity, we h...

journal_title：FEBS letters

authors： Steitz SA,Hasegawa K,Chiang SL,Cobb RR,Castro MA,Lobl TJ,Yamada M,Lazarides E,Cardarelli PM

更新日期：1998-07-03 00:00:00

abstract：:Evidence is presented, by means of both fluorescence and action spectroscopy, that a small, spectroscopically heterogeneous population of both Chl a and Chl b molecules is present in isolated spinach thylakoids and is active in photoinhibition. The broadness of the action spectrum suggests that degraded or incompletel...

journal_title：FEBS letters

authors： Santabarbara S,Neverov KV,Garlaschi FM,Zucchelli G,Jennings RC

更新日期：2001-02-23 00:00:00

abstract：:500 MHz 1H NMR studies of human epidermal growth factor are described. The backbone resonances of the 1-48 derivative of hEGF have been assigned using two-dimensional techniques. Analysis of the type and magnitude of the observed sequential nuclear Overhauser effects and the NH-alpha CH spin-spin coupling constants al...

journal_title：FEBS letters

authors： Carver JA,Cooke RM,Esposito G,Campbell ID,Gregory H,Sheard B

更新日期：1986-09-01 00:00:00

abstract：:We have recently reported a neural variant of microtubule-associated protein 4 with a short pro-rich region (MAP4-SP). Here, we show that the neural MAP4 has reduced microtubule-stabilizing activity, compared to the ubiquitous MAP4 with a long pro-rich region (MAP4-LP), both in vitro and in vivo. Fluorescence recovery...

journal_title：FEBS letters

authors： Hasan MR,Jin M,Matsushima K,Miyamoto S,Kotani S,Nakagawa H

更新日期：2006-06-12 00:00:00

abstract：:An alpha beta heterodimer of the F1-ATPase of Rhodospirillum rubrum was isolated by extraction of chromatophores with LiCl. Each alpha beta heterodimer contains one tightly bound ADP, which is released upon removal of medium Mg2+. The dimer can be reversibly dissociated by removal of Mg(2+)-ions. The alpha beta hetero...

journal_title：FEBS letters

authors： Andralojc PJ,Harris DA

更新日期：1992-09-28 00:00:00

abstract：:Vacuolar tandem-pore channels could not be analysed in Xenopus oocytes so far, due to misguided translocation. Owing to the conservation of their pore regions, we were able to prepare functional pore-chimeras between the plasma membrane localised TPK4 and vacuolar TPKs. Thereby, we found evidence that TPK2, TPK3 and T...

journal_title：FEBS letters

authors： Marcel D,Müller T,Hedrich R,Geiger D

更新日期：2010-06-03 00:00:00

abstract：:Inducible nitric oxide (NO) synthase (iNOS) appears to be a marker of tumor progression in colon carcinogenesis. Here we investigated effects of NO on selected chemokines that differentially regulate angiogenesis, namely pro-angiogenic interleukin (IL)-8 as well as tumor-suppressive interferon-inducible protein-10 (IP...

journal_title：FEBS letters

authors： Hellmuth M,Paulukat J,Ninic R,Pfeilschifter J,Mühl H

更新日期：2004-04-09 00:00:00

abstract：:Stromal cell-derived factor 1alpha (SDF-1alpha) is a chemokine that has been shown to prevent infection of T-tropic HIV strains and is a possible substrate of CD26/dipeptidyl peptidase IV (DPPIV). In this study, we show that SDF-1alpha was cleaved at the N-terminal region by CD26/DPPIV and as a result the inhibitory a...

journal_title：FEBS letters

authors： Ohtsuki T,Hosono O,Kobayashi H,Munakata Y,Souta A,Shioda T,Morimoto C

更新日期：1998-07-17 00:00:00

abstract：:We have isolated a portion of the canine gene encoding the orphan receptor RDC1 [1]. The complete coding sequence is contained in a single exon, and an intron divides the 5' untranslated region of RDC1 mRNA. The RDC1 protein is 94% homologous to the gene product of GPRN1, which has been proposed to serve as a VIP rece...

journal_title：FEBS letters

authors： Cook JS,Wolsing DH,Lameh J,Olson CA,Correa PE,Sadee W,Blumenthal EM,Rosenbaum JS

更新日期：1992-03-30 00:00:00

abstract：:PMA-induced leukotriene C4 synthase (LTC4S) phosphorylation was investigated over a period of 8 h in a monocytic cell line (THP-1). The level of LTC4S phosphorylation was increased 3-5 fold over a 4 h period decreasing to basal levels after 8 h. This phosphorylation event was found to be specific to THP-1 cells as the...

journal_title：FEBS letters

authors： Gupta N,Nicholson DW,Ford-Hutchinson AW

更新日期：1999-04-16 00:00:00