Genetic networks responsive to sodium butyrate in colonic epithelial cells.

abstract：:The transport of proteins from the cytoplasm into the nucleus is a multistep process. The nuclear localization sequence (NLS) of a transport substrate associates with the heterodimeric NLS-receptor which binds to a subset of proteins of the nuclear pore complex (NPC). Translocation through the NPC is energy-dependent ...

journal_title：FEBS letters

authors： Schlenstedt G

更新日期：1996-06-24 00:00:00

abstract：:A one-electron reductive metabolism of 1,2-dibromoethane (DBE) is described that gives rise to a free radical intermediate, which can be stabilized by a spin trapping agent and detected by electron spin resonance spectroscopy. Using rat liver microsomes or isolated hepatocytes from phenobarbitone pretreated animals, u...

journal_title：FEBS letters

authors： Tomasi A,Albano E,Dianzani MU,Slater TF,Vannini V

更新日期：1983-08-22 00:00:00

abstract：:Escherichia coli heat-shock proteins GroEL and GroES stimulate (in an ATP-dependent manner) the folding of various proteins. In this study scanning microcalorimetry was applied to investigate GroEL thermostability in the presence of its ligands. Mg2+ and K+ ions stabilize while ADP destabilizes the GroEL molecule agai...

journal_title：FEBS letters

authors： Surin AK,Kotova NV,Kashparov IA,Marchenkov VV,Marchenkova SYu,Semisotnov GV

更新日期：1997-04-01 00:00:00

abstract：:Adiponectin and resistin, two recently identified adipocyte-specific secretory factors, are able to modulate insulin actions in target tissues. To investigate their expression and hormonal regulation in brown adipocytes, we used the brown adipocyte cell line T37i, which, beside uncoupling protein expression, secretes ...

journal_title：FEBS letters

authors： Viengchareun S,Zennaro MC,Pascual-Le Tallec L,Lombes M

更新日期：2002-12-18 00:00:00

abstract：:Using an all-atom, molecular dynamics-based, flexible docking method, the tertiary and quaternary structures of protofilaments of the "K3" fragment from beta(2)-microglobulin (residues Ser20-Lys41) were predicted at low pH in a continuous mixture of water and 2,2,2-trifluoroethanol (TFE). Tetramers with energies very ...

journal_title：FEBS letters

authors： Standley DM,Yonezawa Y,Goto Y,Nakamura H

更新日期：2006-11-13 00:00:00

abstract：:VP64 is widely used as a transcriptional activator to investigate the biological function of genes, but its potential for application in genetic improvement of crops has not been fully investigated. Here, we characterized an OsMYB1R1-VP64 fusion protein that enhanced the grain yield of rice cultivar 'Kita-ake' by 35%....

journal_title：FEBS letters

authors： Wang J,Wu F,Zhu S,Xu Y,Cheng Z,Wang J,Li C,Sheng P,Zhang H,Cai M,Guo X,Zhang X,Wang C,Wan J

更新日期：2016-10-01 00:00:00

abstract：:The reversible folding/unfolding of a short peptide in solution is studied by molecular dynamics simulations. The effects of long-range electrostatic interactions are examined and found to be important both for the equilibrium between folded and unfolded states and the dynamics of the folding process. The neglect of l...

journal_title：FEBS letters

authors： Aqvist J

更新日期：1999-09-03 00:00:00

abstract：:The active A1 peptide of cholera toxin was linked by a disulphide bond to the lectin of Wisteria floribunda. The resulting conjugate activated the adenylate cyclase of intact U937 or K562 cells at the same concentrations as native toxin did, but to a greater extent. Activation was inhibited by N-acetyl-D-galactosamine...

journal_title：FEBS letters

authors： van Heyningen S

更新日期：1983-11-28 00:00:00

abstract：:Phospholipase A2 has been purified from the venom of Horned viper (Cerastes cerastes) by gel permeation chromatography followed by reverse-phase HPLC. The primary structure was established by sequence analysis of the intact protein and its enzymic peptides. The structure has 120 residues, properties like other group I...

journal_title：FEBS letters

authors： Siddiqi AR,Shafqat J,Zaidi ZH,Jörnvall H

更新日期：1991-01-14 00:00:00

abstract：:Myotonic dystrophy protein kinase (DMPK) is a serine-threonine protein kinase encoded by the myotonic dystrophy (DM) locus on human chromosome 19q13.3. It is a close relative of other kinases that interact with members of the Rho family of small GTPases. We show here that the actin cytoskeleton-linked GTPase Rac-1 bin...

journal_title：FEBS letters

authors： Shimizu M,Wang W,Walch ET,Dunne PW,Epstein HF

更新日期：2000-06-23 00:00:00

abstract：:Gap junctions (GJs) traverse apposing membranes of neighboring cells to mediate intercellular communication by passive diffusion of signaling molecules. We have shown previously that cells endocytose GJs utilizing the clathrin machinery. Endocytosis generates cytoplasmic double-membrane vesicles termed annular gap jun...

journal_title：FEBS letters

authors： Fong JT,Nimlamool W,Falk MM

更新日期：2014-03-03 00:00:00

abstract：:Large amounts of refined sequence material in the form of predicted, curated and annotated genes and expressed sequences tags (ESTs) have recently been added to the NCBI databases. We matched the transcript-sequences of RefSeq, Ensembl and dbEST in an attempt to provide an updated overview of how many unique human gen...

journal_title：FEBS letters

authors： Larsson TP,Murray CG,Hill T,Fredriksson R,Schiöth HB

更新日期：2005-01-31 00:00:00

abstract：:We have previously reported that Monad, a novel WD40 repeat protein, potentiates apoptosis induced by tumor necrosis factor-alpha(TNF-alpha) and cycloheximide (CHX). By affinity purification and mass spectrometry, we identified RNA polymerase II-associated protein 3 (RPAP3) as a binding protein of Monad. Overexpressio...

journal_title：FEBS letters

authors： Itsuki Y,Saeki M,Nakahara H,Egusa H,Irie Y,Terao Y,Kawabata S,Yatani H,Kamisaki Y

更新日期：2008-07-09 00:00:00

abstract：:An interpretation of alternative crystal structures of the erythropoietin receptor, with and without ligand, led to the proposal of a scissor-like mechanism of activation. This model has been propagated in the literature and is still being used to interpret crystal structures of related type-I cytokine receptors. Here...

journal_title：FEBS letters

authors： Corbett MS,Poger D,Mark AE

更新日期：2016-09-01 00:00:00

abstract：:The effect of the inositol phospholipid-binding antibiotic neomycin was studied on high-affinity GTPase in human platelet membranes. At low concentrations (up to 1 mM), neomycin by itself stimulated a high-affinity GTPase. This GTPase stimulation was additive with that caused by the hormonal factors, prostaglandin E1 ...

journal_title：FEBS letters

authors： Herrmann E,Jakobs KH

更新日期：1988-02-29 00:00:00

abstract：:Yeast genes involved in thiamin pyrophosphate (TPP) synthesis are transcriptionally induced in response to thiamin starvation. In this system, three proteins (Thi2p, Thi3p, and Pdc2p) act as positive regulatory factors. Thi3p is a TPP-binding protein and upregulates THI genes expression when TPP is not bound. We found...

journal_title：FEBS letters

authors： Nosaka K,Onozuka M,Konno H,Akaji K

更新日期：2008-12-10 00:00:00

abstract：:A selective PKC inhibitor, UCN-01, was shown to exhibit anti-tumor activity in vitro and in vivo. We investigated UCN-01 with respect to isozyme-specific PKC inhibition using purified recombinant or rabbit brain PKC isozymes, cPKC alpha, beta and gamma, nPKC delta, epsilon and eta, and a PKC zeta. Of the PKC isozymes ...

journal_title：FEBS letters

authors： Mizuno K,Noda K,Ueda Y,Hanaki H,Saido TC,Ikuta T,Kuroki T,Tamaoki T,Hirai S,Osada S

更新日期：1995-02-13 00:00:00

abstract：:When MCF-7 cells were treated with 17 beta-estradiol, dexamethasone, or promegestone at 37 degrees C, the KD of receptors for their cognate ligands was found to decrease as compared to that measured at 2 degrees C. Cell incubation with hormone at 37 degrees C did not affect the Bmax of glucocorticoid and progesterone ...

journal_title：FEBS letters

authors： Rossini GP,Malaguti C

更新日期：1995-12-04 00:00:00

abstract：:The retromer complex is a key element of the endosomal protein sorting machinery that is conserved through evolution and has been shown to play a role in diseases such as Alzheimer's disease and Parkinson's disease. Through sorting various membrane proteins (cargo), the function of retromer complex has been linked to ...

journal_title：FEBS letters

authors： Mukadam AS,Seaman MN

更新日期：2015-09-14 00:00:00

abstract：:EcL-DER, the aspartate/glutamate racemase from the pathogen Escherichia coli O157, exhibits racemase activity for l-aspartate and l-glutamate. This study reports the crystal structures of apo-EcL-DER, the EcL-DER-l-aspartate and the EcL-DER-d-aspartate complexes. The EcL-DER structure contains two domains, forming pse...

journal_title：FEBS letters

authors： Liu X,Gao F,Ma Y,Liu S,Cui Y,Yuan Z,Kang X

更新日期：2016-04-01 00:00:00

abstract：:Effects of amino acid substitutions in the first extracellular loop region of the delta- and mu-opioid receptors were examined. Substitution of lysine-108 of the delta-receptor (delta K108) with asparagine improved affinity to [D-Ala2,MePhe4,Gly-ol5]enk ephalin (DAGO), a mu-selective peptide agonist, to be comparable ...

journal_title：FEBS letters

authors： Fukuda K,Terasako K,Kato S,Mori K

更新日期：1995-10-09 00:00:00

abstract：:Biochemical, immunohistochemical and molecular biological methods were used to detect fetal myosin heavy chain (MyHC) in the human masseter of elderly and young subjects. Samples from the elderly subjects contained larger amounts of fetal MyHC than those of young adults. Only a very small amount of embryonic MyHC coul...

journal_title：FEBS letters

authors： Monemi M,Eriksson PO,Dubail I,Butler-Browne GS,Thornell LE

更新日期：1996-05-13 00:00:00

abstract：:We amplified, using the polymerase chain reaction and calcitonin receptor (CTR) specific primers, RNA extracted from medullary thyroid carcinoma (MTC) and the derived TT cell line. Both secrete large amounts of calcitonin. Electrophoresis of amplification products revealed, in both cases, an ethidium bromide-stained b...

journal_title：FEBS letters

authors： Frendo JL,Pichaud F,Mourroux RD,Bouizar Z,Segond N,Moukhtar MS,Jullienne A

更新日期：1994-04-04 00:00:00

abstract：:Complete [1H]NMR resonance assignments are presented for the second of the three zinc fingers from SW15 as it appears in both 1- and 2-finger constructs. Signals from finger 2 are unaffected by the presence or absence of finger 1, showing that the protein has a modular construction. The structure of finger 2 comprises...

journal_title：FEBS letters

authors： Neuhaus D,Nakaseko Y,Nagai K,Klug A

更新日期：1990-03-26 00:00:00

abstract：:Several protein kinase C (PKC) isozymes were analyzed by immunoblot and immunocytochemistry in cultures of hippocampal neurones at several stages of differentiation. Our findings reveal the existence of two distinct patterns of expression. Firstly, conventional PKC isozymes alpha, beta and gamma, that are expressed at...

journal_title：FEBS letters

authors： Tejero-Díez P,Rodríguez-Sánchez P,Díez-Guerra FJ

更新日期：1995-04-24 00:00:00

abstract：:The crawling locomotion and shape of eukaryotic cells have been associated with the stochastic molecular dynamics of actin and its protein regulators, chiefly Arp2/3 and Rho family GTPases, in making a cytoskeleton meshwork within cell extensions. However, the cell's actin-dependent oscillatory shape and extension dyn...

journal_title：FEBS letters

authors： Vicker MG

更新日期：2002-01-02 00:00:00

abstract：:Pathway analysis methods are frequently applied to cancer gene expression data to identify dysregulated pathways. These methods often infer pathway activity based on the expression of genes belonging to a given pathway, even though the proteins ultimately determine the activity of a given pathway. Furthermore, the ass...

journal_title：FEBS letters

authors： Szalai B,Saez-Rodriguez J

更新日期：2020-12-01 00:00:00

abstract：:The ABCG2 protein has a key role in the transport of a wide range of structurally dissimilar endo- and xenobiotics in the human body, especially in the tissue barriers and the metabolizing or secreting organs. The human ABCG2 gene harbors a high number of polymorphisms and mutations, which may significantly modulate i...

journal_title：FEBS letters

authors： Sarkadi B,Homolya L,Hegedűs T

更新日期：2020-10-04 00:00:00

abstract：:Lipoic acid is a coenzyme essential to the activity of enzymes such as pyruvate dehydrogenase, which play important roles in central metabolism. However, neither the enzymes responsible for biosynthesis nor the biosynthetic event of lipoic acid has been reported in mammalian cells. In this study, a mouse mLIP1 cDNA fo...

journal_title：FEBS letters

authors： Morikawa T,Yasuno R,Wada H

更新日期：2001-06-01 00:00:00

abstract：:Study of the relative proportions of ribosomes and polysomes released by a standardized lysing procedure from isogenic RC(str) and RC(rel) strains of Escherichia coli shows that a 20-min period of amino acid starvation of RC(str) bacteria reduces the fraction of ribosomes recovered in polysomes to about 60% of its val...

journal_title：FEBS letters

authors： Matzura H

更新日期：1970-01-15 00:00:00