Metabolic activation of 1,2-dibromoethane to a free radical intermediate by rat liver microsomes and isolated hepatocytes.

abstract：:The present study provides the first evidence that the abundance of catalytic alpha1-subunit of Na,K-ATPase increases in the course of T cell blast transformation. Immunodepressant cyclosporin A at anti-proliferative doses diminished the induction of alpha1 protein in activated lymphocytes. Furthermore, in competent T...

journal_title：FEBS letters

authors： Marakhova I,Karitskaya I,Aksenov N,Zenin V,Vinogradova T

更新日期：2005-05-23 00:00:00

abstract：:The E-E* model for calcium pumping by the CaATPase of sarcoplasmic reticulum includes two distinct conformational states of the enzyme, E and E*. Exterior Ca2+ binds only to E and interior Ca2+ binds only to E*. Therefore, it is expected that there will be competition between the binding of calcium to the unphosphoryl...

journal_title：FEBS letters

authors： Myung J,Jencks WP

更新日期：1991-01-14 00:00:00

abstract：:On the basis of recent results, we propose a model for DNA polymerase translocation along DNA. Human immunodeficiency virus reverse transcriptase is taken as an example. According to the model, movement of the enzyme is the result of transition of the enzyme-bound DNA from the A- to B-form which is accompanied by leng...

journal_title：FEBS letters

authors： Wlassoff WA,Dymshits GM,Lavrik OI

更新日期：1996-07-15 00:00:00

abstract：:The NAD+-requiring enzymes of glycoside hydrolase family 4 (GHF4) contain a region with a conserved Gly-XXX-Gly-Ser (GXGS) motif near their N-termini that is reminiscent of the fingerprint region of the Rossmann fold, a conserved structural motif of classical nicotinamide nucleotide-binding proteins. The function of t...

journal_title：FEBS letters

authors： Raasch C,Armbrecht M,Streit W,Höcker B,Sträter N,Liebl W

更新日期：2002-04-24 00:00:00

abstract：:Interaction of the water oxidising manganese complex of photosystem II with the aqueous environment has been investigated using electron paramagnetic resonance spectroscopy and electron spin echo envelope modulation spectroscopy to detect interaction of [2H]methanol with the complex in the S2 state. The experiments sh...

journal_title：FEBS letters

authors： Evans MC,Gourovskaya K,Nugent JH

更新日期：1999-05-07 00:00:00

abstract：:C26, the C-terminal 26 residue peptide of serpin A1, significantly increased cell proliferation in cultures of hepatoma cells, but not in porcine kidney epithelial cells, human skin fibroblasts or keratinocytes. The mitogenic activity of C26 was preferentially inhibited with a protein kinase C (PKC) inhibitor, an anti...

journal_title：FEBS letters

authors： Congote LF,Temmel N

更新日期：2004-10-22 00:00:00

abstract：:Using antibodies recognizing the phosphorylation state of specific sites, phosphorylation states of tau were monitored in fetal rat primary cultured neurons. When cultured neurons were treated with okadaic acid (OA) or calyculin A (CalA) at concentrations sufficient to inhibit protein phosphatase 2A (PP2A), phosphoryl...

journal_title：FEBS letters

authors： Saito T,Ishiguro K,Uchida T,Miyamoto E,Kishimoto T,Hisanaga S

更新日期：1995-12-04 00:00:00

abstract：:A collagenase cleaving native type I [14C]collagen but inactive against the synthetic substrate Pz-Pro-Leu-Gly-Pro-D-Arg was extracted from mineralized human dental tissue. The enzyme specifically degrades native collagen into characteristic products (3/4) and (1/4). Its apparent molecular mass of 68 kDa is relatively...

journal_title：FEBS letters

authors： Dumas J,Hurion N,Weill R,Keil B

更新日期：1985-07-22 00:00:00

abstract：:SV40 large T antigen is phosphorylated at up to ten different amino acids clustered in an N-terminal and a C-terminal part of the polypeptide chain. The N-terminal phosphorylated residues include Ser 123 and Thr 124. We have analyzed the oligomerization, the complex formation with the cellular oncoprotein p53 and the ...

journal_title：FEBS letters

authors： Montenarh M,Müller D

更新日期：1987-09-14 00:00:00

abstract：:Cav3.2 T-type Ca(2+) channel is required for the activation of calcineurin/NFAT signaling in cardiac hypertrophy. We aimed to investigate how Cav3.2 and calcineurin interact. We found that Ca(2+) and calmodulin modulate the Cav3.2/calcineurin interaction. Calcineurin binding to Cav3.2 decreases the enzyme's phosphatas...

journal_title：FEBS letters

authors： Huang CH,Chen YC,Chen CC

更新日期：2013-06-19 00:00:00

abstract：:PTPRO is often silenced by DNA hypermethylation in primary human tumors and cancer cell lines and functions as a tumor suppressor. Here we show that PTPRO is a target of E2F1. In addition, the microRNA cluster miR-17-92, another target of E2F1, participates in PTPRO regulation. PTPRO mRNA was up-regulated during S pha...

journal_title：FEBS letters

authors： Xu X,Hong Y,Kong C,Xu L,Tan J,Liang Q,Huang B,Lu J

更新日期：2008-08-20 00:00:00

abstract：:Monoamine oxidase B (MAO B) is an outer mitochondrial membrane protein that oxidizes arylalkylamine neurotransmitters and has been a valuable drug target for many neurological disorders. The 1.7 angstrom resolution structure of human MAO B shows the enzyme is dimeric with a C-terminal transmembrane helix protruding fr...

journal_title：FEBS letters

authors： Binda C,Hubálek F,Li M,Edmondson DE,Mattevi A

更新日期：2004-04-30 00:00:00

abstract：:Despite significant increases in circulating concentrations of lipid fuels (triacylglycerol, non-esterified fatty acids (NEFA) and ketone bodies) in late-pregnant rats sampled in the fed (absorptive) state, cardiac and skeletal muscle active pyruvate dehydrogenase (PDHa) activities remained comparable with those obser...

journal_title：FEBS letters

authors： Sugden MC,Holness MJ

更新日期：1993-04-26 00:00:00

abstract：:A key step in sulfate assimilation into cysteine is the reduction of sulfite to sulfide by sulfite reductase (SiR). This enzyme is encoded by three genes in the moss Physcomitrella patens. To obtain a first insight into the roles of the individual isoforms, we deleted the gene encoding the SiR1 isoform in P. patens by...

journal_title：FEBS letters

authors： Wiedemann G,Hermsen C,Melzer M,Büttner-Mainik A,Rennenberg H,Reski R,Kopriva S

更新日期：2010-06-03 00:00:00

abstract：:The conformation change of Thermus thermophilus tRNA(1Ile) upon complex formation with T. thermophilus elongation factor Tu (EF-Tu) was studied by analysis of the circular dichroism (CD) bands at 315 nm (due to the 2-thioribothymidine residue in the T-loop) and at 295 nm (due to the core structure of tRNA). Formation ...

journal_title：FEBS letters

authors： Haruki M,Matsumoto R,Hara-Yokoyama M,Miyazawa T,Yokoyama S

更新日期：1990-04-24 00:00:00

abstract：:The main immunogenic region (MIR) of the acetylcholine receptor (AChR) is the target for the majority of high-affinity autoantibodies produced in myasthenia gravis patients. Some monoclonal antibodies (mAbs) to the MIR bind specifically, but with low affinity, to synthetic AChR alpha subunit peptides with the sequence...

journal_title：FEBS letters

authors： Saedi MS,Anand R,Conroy WG,Lindstrom J

更新日期：1990-07-02 00:00:00

abstract：:The homeoprotein Engrailed (Engrailed-1/Engrailed-2, collectively En1/2) is not only a survival factor for mesencephalic dopaminergic (mDA) neurons during development, but continues to exert neuroprotective and physiological functions in adult mDA neurons. Loss of one En1 allele in the mouse leads to progressive demis...

journal_title：FEBS letters

authors： Rekaik H,Blaudin de Thé FX,Prochiantz A,Fuchs J,Joshi RL

更新日期：2015-12-21 00:00:00

abstract：:Myogenesis is conducted by transcription factors including MyoD and myogenin. Myogenin is known to be polyubiquitinated by SCF (Skp1/Cullin 1/F-box protein) followed by proteasomal degradation, though the participating F-box protein is remaining unidentified. In this study, we found that myogenin in differentiated myo...

journal_title：FEBS letters

authors： Jogo M,Shiraishi S,Tamura TA

更新日期：2009-09-03 00:00:00

abstract：:AtCYP20-2 is one of 16 immunophilins in thylakoid lumen. The presence of the isomerase domain in AtCYP20-2, an enrichment of AtCYP20-2 in the stroma membranes and it's co-migration with NAD(P)H dehydrogenase (NDH) in native gels provide evidence that AtCYP20-2 is an auxiliary protein of NDH. When different NDH mutants...

journal_title：FEBS letters

authors： Sirpiö S,Holmström M,Battchikova N,Aro EM

更新日期：2009-07-21 00:00:00

abstract：:The effects of progesterone, dehydroepiandrosterone sulfate and 17beta-estradiol on the synthesis and degradation of hyaluronate were investigated using human uterine cervix fibroblasts. The cells were incubated with [3H]glucosamine in the presence of the hormones and then [3H]hyaluronate was isolated from the medium....

journal_title：FEBS letters

authors： Tanaka K,Nakamura T,Takagaki K,Funahashi M,Saito Y,Endo M

更新日期：1997-02-03 00:00:00

abstract：:Stability of green fluorescent protein (GFP) is sometimes important for a proper practical application of this protein. Random mutagenesis and targeted mutagenesis have been used to create better-folded variants of GFP, including recently reported extra-superfolder GFP. Our aim was to determine the crystal structure o...

journal_title：FEBS letters

authors： Choi JY,Jang TH,Park HH

更新日期：2017-01-01 00:00:00

abstract：:Changes in the ADP-ribosylation of total proteins and purified histones of rat liver nuclei after phenobarbital treatment (80 mg/kg, 24 h) have been studied. The [32P]NAD incorporation into total trichloroacetic acid precipitated proteins, in histone Hl and in core histones was evaluated, the specific radioactivities ...

journal_title：FEBS letters

authors： Bràz J,Lechner MC

更新日期：1986-04-21 00:00:00

abstract：:Polymyxin B (PXB), a cyclic peptide antibiotic, in concentrations 0.1-3.0 mg/ml (0.08-4.0 mmol/l), inhibited the K+ efflux induced by opening of the Ca2+-activated K+ channel (the Gárdos effect) in intact human red blood cells. The inhibition was observed when the Gárdos effect was elicited by Ca2+ in the presence of ...

journal_title：FEBS letters

authors： Varecka L,Peterajová E,Pogády J

更新日期：1987-12-10 00:00:00

abstract：:Aspergillus nidulans catalase B (CatB) was purified to homogeneity and characterized as a hydroperoxidase which resembles typical catalases in some physicochemical characteristics: (1) it has an apparent molecular weight of 360000 and is composed of four glycosylated subunits, (2) it has hydrophobic properties as reve...

journal_title：FEBS letters

authors： Calera JA,Sánchez-Weatherby J,López-Medrano R,Leal F

更新日期：2000-06-16 00:00:00

abstract：:In order to get a better insight into the function of amino acid residues located in the second transmembrane domain of the cystic fibrosis transmembrane conductance regulator (CFTR) protein, all exon 18 mutations found in cystic fibrosis (CF) patients were characterized at the protein and at the electrophysiological ...

journal_title：FEBS letters

authors： Vankeerberghen A,Wei L,Teng H,Jaspers M,Cassiman JJ,Nilius B,Cuppens H

更新日期：1998-10-16 00:00:00

abstract：:Interactions of U2 snRNA with anti-Sm precipitable proteins in RNA-free cytoplasmic complexes were analyzed. U2 snRNA was found to bind specifically with proteins in the 6S complexes but not in the 20S complexes. The binding activity was preserved using U2 snRNA having a mutated Sm binding site. Label-transfer experim...

journal_title：FEBS letters

authors： Fury MG,Andersen J

更新日期：1997-03-03 00:00:00

abstract：:The mechanism of the maize pyruvate, phosphate dikinase-catalyzed conversion of adenosine 5'-triphosphate, orthophosphate and pyruvate to adenosine 5'-monophosphate, inorganic pyrophosphate and phosphoenolpyruvate, respectively, was determined by using transient kinetic techniques. The data obtained demonstrate that c...

journal_title：FEBS letters

authors： Carroll LJ,Dunaway-Mariano D,Smith CM,Chollet R

更新日期：1990-11-12 00:00:00

abstract：:PsbY is one of the low molecular mass subunits of oxygen-evolving photosystem II (PSII). Its location, however, has not been identified in the current crystal structure of PSII. We constructed a PsbY-deletion mutant of Thermosynechococcus elongatus, crystallized, and analyzed the crystal structure of the mutant PSII d...

journal_title：FEBS letters

authors： Kawakami K,Iwai M,Ikeuchi M,Kamiya N,Shen JR

更新日期：2007-10-16 00:00:00

abstract：:As the initiator of DNA double-strand breaks during meiosis in Saccharomyces cerevisiae, the SPO11 protein is essential for recombination. Similarity between SPO11 and archaebacterial TOP6A proteins points to evolutionary specialization of a DNA cleavage function for meiotic recombination. To determine whether this ex...

journal_title：FEBS letters

authors： Shannon M,Richardson L,Christian A,Handel MA,Thelen MP

更新日期：1999-12-03 00:00:00

abstract：:Sodium azide inhibited multi-site (steady-state) ATPase activity of E. coli F1 more than 90%, but did not affect uni-site (single-site) ATPase activity. Thus azide inhibited multi-site ATPase activity by lowering catalytic cooperativity. Consistent with this observation, azide changed the ligand-induced fluorescence r...

journal_title：FEBS letters

authors： Noumi T,Maeda M,Futai M

更新日期：1987-03-23 00:00:00