Identification of MAFbx as a myogenin-engaged F-box protein in SCF ubiquitin ligase.

abstract：:Bacterial RNA polymerase (RNAP) serves as a primase during replication of single-stranded plasmids and filamentous phages. Primer RNA (prRNA) synthesis from the origin regions of these replicons depends on the σ factor that normally participates in promoter recognition. However, it was proposed that σ may not be requi...

journal_title：FEBS letters

authors： Esyunina D,Pupov D,Kulbachinskiy A

更新日期：2019-02-01 00:00:00

abstract：:In the rat liver, transcription of the serine:pyruvate/alanine:glyoxylate aminotransferase (SPT/AGT) gene occurs from two sites, +1 and +66, in exon 1, resulting in the formation of two mRNAs, one for a precursor of mitochondrial SPT/AGT and the other for peroxisomal SPT/AGT, respectively. In this study, we attempted ...

journal_title：FEBS letters

authors： Sugiyama T,Uchida C,Oda T,Kitagawa M,Hayashi H,Ichiyama A

更新日期：2001-11-09 00:00:00

abstract：:In order to improve our understanding of membrane protein solubilization by sodium dodecylsulphate, sarcoplasmic reticulum vesicles have been treated with this surfactant at different detergent: protein mole ratios. Effects on Ca2(+)-ATPase activity, membrane protein solubilization, and protein conformation have been ...

journal_title：FEBS letters

authors： Prado A,Muga A,Castresana J,Goñi FM,Arrondo JL

更新日期：1990-09-03 00:00:00

abstract：:Helical filaments of bacterial flagella are built up by a self-assembly process from thousands of flagellin subunits. To clarify how the disordered terminal regions of flagellin interact upon filament formation, polymerization ability of various terminally truncated fragments was investigated. Fragments deprived of 19...

journal_title：FEBS letters

authors： Gugolya Z,Muskotál A,Sebestyén A,Diószeghy Z,Vonderviszt F

更新日期：2003-01-30 00:00:00

abstract：:Two clones were isolated by screening a shrimp hepatopancreas cDNA library with a DNA fragment obtained by PCR amplification using two oligonucleotides based on the partial protein sequence of Penaeus vanameii chymotrypsin purified earlier. One of these clones, PVC 7 contains a complete cDNA coding for a serine protea...

journal_title：FEBS letters

authors： Sellos D,Van Wormhoudt A

更新日期：1992-09-14 00:00:00

abstract：:An Arabidopsis cDNA coding for a previously uncharacterized isoform of inorganic pyrophosphatase was isolated. It was used to complement an E. coli mutant, demonstrating that it coded for an active enzyme. MgCl(2) was necessary for the protein's activity, whilst NaF inhibited it. The K(m) for pyrophosphate and the pH ...

journal_title：FEBS letters

authors： Schulze S,Mant A,Kossmann J,Lloyd JR

更新日期：2004-05-07 00:00:00

abstract：:A short conserved sequence equivalent to the fifth conserved sequence region of alpha-amylases (173_LPDLD, Aspergillus oryzae alpha-amylase) comprising the calcium-ligand aspartate, Asp-175, was identified in the amino acid sequences of several members of the family of (alpha/beta)8-barrel glycosyl hydrolases. Despite...

journal_title：FEBS letters

authors： Janecek S

更新日期：1995-12-11 00:00:00

abstract：:Automatic design is based on computational modeling and optimization methods to provide prototype designs to targeted problems in an unsupervised manner. For biological circuits, we need to produce quantitative predictions of cell behavior for a given genotype as consequence of the different molecular interactions. Au...

journal_title：FEBS letters

authors： Rodrigo G,Carrera J,Landrain TE,Jaramillo A

更新日期：2012-07-16 00:00:00

abstract：:The type 1 ribosome-inactivating protein (RIP) saporin 5 isolated from seeds of Saponaria officinalis L. strongly inhibited translation carried out by Vicia sativa L. purified ribosomes. The toxin multidepurinated V. sativa rRNA, which upon treatment with acid aniline releases several RNA fragments including an RNA fr...

journal_title：FEBS letters

authors： Iglesias R,Arias FJ,Rojo MA,Escarmis C,Ferreras JM,Girbés T

更新日期：1993-07-05 00:00:00

abstract：:The human mutT homolog, hMTH1, suppresses spontaneous mutations by degrading the endogeneous mutagen, 8-hydroxy-dGTP. We previously reported the broad substrate specificity of hMTH1, which also degrades the oxidatively damaged purine nucleotides, 2-hydroxy-dATP, 8-hydroxy-dATP, 2-hydroxy-ATP, and 8-hydroxy-GTP, in add...

journal_title：FEBS letters

authors： Fujikawa K,Yakushiji H,Nakabeppu Y,Suzuki T,Masuda M,Ohshima H,Kasai H

更新日期：2002-02-13 00:00:00

abstract：:Guanylate cyclase activity was measured in disrupted rod outer segments of the toad retina. The experiments showed that cGMP is synthesized from GTP at a rate of 3 +/- 1 nmol/min per mg protein. In darkness this value is largely independent of the Ca2+ concentration, while it is enhanced by flashes of light of increas...

journal_title：FEBS letters

authors： Pepe IM,Panfoli I,Cugnoli C

更新日期：1986-07-14 00:00:00

abstract：:Thrombin has been shown to inhibit skeletal muscle differentiation. However, the mechanisms by which thrombin represses myogenesis remain unknown. Since the thrombin receptor couples to G(i), G(q/11) and G(12), we examined which subunits of heterotrimeric guanine nucleotide-binding regulatory proteins (Galpha(i), Galp...

journal_title：FEBS letters

authors： Nagao M,Kaziro Y,Itoh H

更新日期：2000-04-28 00:00:00

abstract：:The lateral diffusion of BK(Ca) channels was previously shown to be highly 'confined' in the COS-7 cell membrane. Here we report that the diffusion coefficient and the confinement area of BK(Ca) channel were significantly increased by the treatment of latrunculin A, an actin-depolymerizing agent, but not by microtubul...

journal_title：FEBS letters

authors： Won S,Lee BC,Park CS

更新日期：2011-07-21 00:00:00

abstract：:Re-analysis of the primary structure of the ribosomal RNA N-methyltransferase that confers self-resistance on the erythromycin-producing bacterium Saccharopolyspora erythraea has confirmed the presence of a C-terminal domain containing extensive repeat sequences. Nine tandem repeats can be discerned, with a decapeptid...

journal_title：FEBS letters

authors： Dhillon N,Leadlay PF

更新日期：1990-03-26 00:00:00

abstract：:CD38, a type II transmembrane glycoprotein, behaves as a catalytically active transporter responsible for ectocellular generation of cyclic ADP-ribose (cADPR) from NAD+ and for subsequent influx of cADPR across membranes [Franco, L., Guida, L., Bruzzone, S., Zocchi, E., Usai, C. and De Flora, A. (1998) FASEB J. in pre...

journal_title：FEBS letters

authors： Bruzzone S,Guida L,Franco L,Zocchi E,Corte G,De Flora A

更新日期：1998-08-21 00:00:00

abstract：:A new enzyme catalyzing the deamidation of seed storage proteins was found in germinating wheat grains and was partially purified. It also acts on egg lysozyme, horse hemoglobin and reduced RNAse, glutamine and Gly-L-Gln-L-Tyr. No activity was observed when using ovalbumin, serum albumin, RNAse, insulin, asparagine an...

journal_title：FEBS letters

authors： Vaintraub IA,Kotova LV,Shaha R

更新日期：1992-05-11 00:00:00

abstract：:Mitochondrial translocation of ceramides triggers Bax-dependent apoptosis. To elucidate how ceramides activate Bax and commit cells to death, we developed a switchable version of the ceramide transfer protein CERT, sCERT. Upon its drug-induced recruitment to mitochondria, sCERT retains the ability to bind VAP proteins...

journal_title：FEBS letters

authors： Jain A,Dadsena S,Holthuis JCM

更新日期：2020-11-01 00:00:00

abstract：:Cellular homeostasis in response to glucose availability is maintained through the tight coordination of various physiological processes, including cell proliferation, transcription, and metabolism. In this study, we use the budding yeast Saccharomyces cerevisiae to identify proteins implicated in carbon source-depend...

journal_title：FEBS letters

authors： Umekawa M,Shiraishi D,Fuwa M,Sawaguchi K,Mashima Y,Katayama T,Karita S

更新日期：2020-04-01 00:00:00

abstract：:The possible participation of calmodulin in the activation of store-operated Ca2+ entry (SOC) in single rat hepatocytes was investigated microspectrofluorimetrically. SOC was triggered after discharging intracellular Ca2+ stores using the endoplasmic reticulum Ca2+-ATPase inhibitor thapsigargin in the absence of exter...

journal_title：FEBS letters

authors： Cao Y,Chatton JY

更新日期：1998-03-06 00:00:00

abstract：:The interferon inducible transmembrane (IFITM) proteins mediate several cellular processes such as homotypic cell adhesion functions of interferons (IFNs) and cellular anti-proliferative activities. We show that the BAF complex-mediated induction of IFITM3 is dependent on binding of the transcriptional enhancer factor...

journal_title：FEBS letters

authors： Cuddapah S,Cui K,Zhao K

更新日期：2008-01-23 00:00:00

abstract：:The biological activity and the fate of SV40 DNA (minichromosomes, DNA I, DNA II, DNA III) were tested in culture cells by immunofluorescence staining and blot analysis. Following microinjection of 2-4 circular SV40 molecules (minichromosomes, DNA I, DNA II) into the cytoplasm or the nuclei of monkey and rat cells, T-...

journal_title：FEBS letters

authors： Graessmann A,Bumke-Vogt C,Buschhausen G,Bauer M,Graessmann M

更新日期：1985-01-01 00:00:00

abstract：:Tumour necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a member of the TNF family of cytokines that promotes apoptosis and NF-kappaB activation. Here we show that recombinant hu-TRAIL initiates the activation of multiple caspases, the loss of mitochondrial transmembrane potential, the cleavage of BI...

journal_title：FEBS letters

authors： Keogh SA,Walczak H,Bouchier-Hayes L,Martin SJ

更新日期：2000-04-07 00:00:00

abstract：:A cDNA coding for the mouse hepatic triglyceride lipase has been isolated from a mouse liver cDNA library. The nucleotide sequence of the cDNA shows an open reading frame encoding a polypeptide of 510 amino acids that is 91.5% and 86% homologous to rat and human hepatic lipase, respectively. The most drastic protein s...

journal_title：FEBS letters

authors： Chang SF,Netter HJ,Will H

更新日期：1991-09-02 00:00:00

abstract：:The mucosal epithelium secretes a variety of antimicrobial peptides that act as part of the innate immune system to protect against invading microbes. Here, we describe the functional properties of human defensin (HD) 5, the major antimicrobial peptide produced by Paneth cells in the ileum, in relation to its structur...

journal_title：FEBS letters

authors： de Leeuw E,Burks SR,Li X,Kao JP,Lu W

更新日期：2007-02-06 00:00:00

abstract：:A gene encoding a single-chain antibody fragment directed against digoxin (named 1C10 scFv) was cloned in two expression systems. For this purpose, a new baculovirus transfer cassette fully compatible with the procaryotic pHEN vector was constructed. Baculovirus production led to higher yield than did Escherichia coli...

journal_title：FEBS letters

authors： Lemeulle C,Chardès T,Montavon C,Chaabihi H,Mani JC,Pugnière M,Cerutti M,Devauchelle G,Pau B,Biard-Piechaczyk M

更新日期：1998-02-20 00:00:00

abstract：:Drosophila PTEN (dPTEN) plays indispensable roles in the development of Drosophila melanogaster by controlling cell size and number. Although three potential spliced forms of dPTEN have been isolated, functional distinction among these forms remains elusive. In this study, we demonstrate that all spliced forms of dPTE...

journal_title：FEBS letters

authors： Maehama T,Kosaka N,Okahara F,Takeuchi K,Umeda M,Dixon JE,Kanaho Y

更新日期：2004-05-07 00:00:00

abstract：:The effect of increasing low density lipoprotein (LDL) concentrations on the synthesis of basement membrane components was investigated in proliferating porcine aortic endothelial cells (PAEC) in culture. Basement membrane-associated heparan sulfate proteoglycan (HSPG) and fibronectin were determined by enzyme immunoa...

journal_title：FEBS letters

authors： Olgemöller B,Schleicher ED,Schwaabe S,Guretzki HJ,Gerbitz KD

更新日期：1990-05-07 00:00:00

abstract：:We synthesized the zwitterionic amphiphile cholesterylphosphorylethylpyridinium. This substance activated protein kinase C (PKC) in a micelle-based assay, but in a vesicle assay it was inhibitory. An analog of this compound, in which the pyridine ring is saturated and the nitrogen methylated, showed similar behaviour ...

journal_title：FEBS letters

authors： Epand RM,Stafford A,Wang J,Epand RF

更新日期：1992-06-15 00:00:00

abstract：:Mild sonication of eukaryotic cells produces native plasma membrane sheets that retain their docked organelles, cytoskeleton structures and cytoplasmic complexes. While the delicate organization of membranous protein complexes remains undisturbed, their inner plasmalemmel leaflet can be rapidly exposed to bathing solu...

journal_title：FEBS letters

authors： Bar-On D,Winter U,Nachliel E,Gutman M,Fasshauer D,Lang T,Ashery U

更新日期：2008-10-15 00:00:00

abstract：:Two genes code for calcitonin gene-related peptides (CGRPs). One expresses by tissue-specific alternate splicing calcitonin and CGRP I mRNAs, the other CGRP II mRNA. Calcitonin is the marker of sporadic or hereditary human medullary thyroid carcinoma (MTC). CGRP II expression is not well established in normal or tumor...

journal_title：FEBS letters

authors： Lasmoles F,Minvielle S,Cohen R,Guliana JM,Delehaye MC,Segond N,Calmettes C,Milhaud G,Moukhtar MS

更新日期：1990-12-17 00:00:00