Abstract:
:A cDNA coding for the mouse hepatic triglyceride lipase has been isolated from a mouse liver cDNA library. The nucleotide sequence of the cDNA shows an open reading frame encoding a polypeptide of 510 amino acids that is 91.5% and 86% homologous to rat and human hepatic lipase, respectively. The most drastic protein sequence divergence is found at the carboxyterminal end which was speculated to harbour one heparin-binding site. By in vitro translation of cRNA in the presence of pancreatic membranes the hepatic lipase was shown to be glycosylated and to have an electrophoretic mobility of 53 kDa.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Chang SF,Netter HJ,Will Hdoi
10.1016/0014-5793(91)80910-usubject
Has Abstractpub_date
1991-09-02 00:00:00pages
69-72issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(91)80910-Ujournal_volume
289pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Jun activation domain-binding protein 1 (Jab1) is a coactivator of activating protein-1 (AP-1) and is the fifth component of the COP9 signalosome complex. It interacts with a variety of proteins and plays important roles in diverse signaling pathways and cellular function including oncogenesis. We show here that Jab1 ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.10.002
更新日期:2006-10-30 00:00:00
abstract::In order to improve our understanding of membrane protein solubilization by sodium dodecylsulphate, sarcoplasmic reticulum vesicles have been treated with this surfactant at different detergent: protein mole ratios. Effects on Ca2(+)-ATPase activity, membrane protein solubilization, and protein conformation have been ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81185-q
更新日期:1990-09-03 00:00:00
abstract::Xenopus oocytes expressing neuronal alpha 1C, alpha 2 and beta 1b calcium channel subunit cDNAs were used in this study. During two-electric voltage clamp recording the oocyte was injected with 10-20 nl of a 100 mM BAPTA solution. Under these conditions, the endogenous Ca-activated Cl current was completely suppressed...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00357-2
更新日期:1994-05-09 00:00:00
abstract::Pigeon liver malic enzyme (EC 1.1.1.40) has a double dimer quaternary structure. The NADP+ analogs, aminopyridine adenine dinucleotide phosphate and nicotinamide-1,N6-ethenoadenosine dinucleotide phosphate, bind to the enzyme anti-cooperatively. In the presence of non-cooperative competing ligand NADP+, the binding pa...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80837-9
更新日期:1990-12-17 00:00:00
abstract::Sodium azide inhibited multi-site (steady-state) ATPase activity of E. coli F1 more than 90%, but did not affect uni-site (single-site) ATPase activity. Thus azide inhibited multi-site ATPase activity by lowering catalytic cooperativity. Consistent with this observation, azide changed the ligand-induced fluorescence r...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81526-0
更新日期:1987-03-23 00:00:00
abstract::Apoptosis represents a fundamental process during fetal/post-natal testis development. Therefore pro- and anti-apoptotic proteins are essential to regulate testis physiology. c-Flip(L) is a known inhibitor of caspase 8/10 activity; in this study its perinatal expression in mouse male germ cells was investigated. In te...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.10.010
更新日期:2006-11-13 00:00:00
abstract::The pH dependence of spin-spin coupling constants J (P-O-P) of ADP and ATP has been determined from the phosphorus NMR spectra at 0 degrees. The variation curves have been interpreted as titration curves. These give different pK values. Comparison with the tripolyphosphate shows the existence of triphosphate chain str...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(70)80243-5
更新日期:1970-06-01 00:00:00
abstract::The amino acid sequence and part of the DNA sequence of a glutamic acid-specific serine protease from Streptomyces griseus is reported. This protease is shown to be homologous with other serine proteases. An improved purification protocol for this enzyme is described. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80859-2
更新日期:1991-11-04 00:00:00
abstract::Interleukin-22 (IL-22) plays an important role in the regulation of immune and inflammatory responses in mammals. The IL-22 binding protein (IL-22BP), a soluble receptor that specifically binds IL-22, prevents the IL-22/interleukin-22 receptor 1 (IL-22R1)/interleukin-10 receptor 2 (IL-10R2) complex assembly and blocks...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.03.006
更新日期:2009-04-02 00:00:00
abstract::This paper summarizes results of the 40-year studies on replication and recombination of RNA molecules in the cell-free amplification system of bacteriophage Q. Special attention is paid to the molecular colony technique that has provided for the discovery of the nature of "spontaneous" RNA synthesis by Q replicase an...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2004.03.066
更新日期:2004-06-01 00:00:00
abstract::Constitutively active tyrosine kinases are frequently expressed in various types of human leukemias as the result of chromosomal translocations. The TEL-Jak2 fusion oncoprotein possesses transforming properties in both animal and cellular models, that are tightly dependent on Stat5 activation. In the IL-3-independent ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02452-8
更新日期:2001-05-25 00:00:00
abstract::WT1 was isolated as a tumor suppressor gene of Wilms tumor. However, high expression of WT1 correlates with poor prognosis in acute leukemia. In addition suppression of WT1 expression by WT1 anti-sense oligonucleotide inhibits proliferation of leukemia cells, suggesting that WT1 is important for their proliferation. T...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00477-8
更新日期:1997-06-02 00:00:00
abstract::At alpha 1-adrenergic receptors in isolated rat liver parenchymal cells, (-)-epinephrine is potent in eliciting a maximal increase in glycogenolysis (Kact = 24 nM). This contrasts with a 100-fold lower affinity for the agonist at alpha 1-adrenergic receptors of intact hepatocytes determined from equilibrium competitio...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81243-6
更新日期:1985-08-05 00:00:00
abstract::The KDEL receptor is a seven-transmembrane-domain protein that was first described about 20 years ago. Its well-known function is to retrotransport chaperones from the Golgi complex to the endoplasmic reticulum. Recent studies, however, have suggested that the KDEL receptor has additional functions. Indeed, we have de...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2009.10.053
更新日期:2009-12-03 00:00:00
abstract::The TSH-induced cyclic AMP response was studied using a 3-year-old ovine thyroid cell line TSH-independent for growth: OVNIS 5H. The kinetics of cyclic AMP production was followed both in cell layers and in cell culture media, with or without phosphodiesterase inhibitor. It is noteworthy that following the first wave ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80102-8
更新日期:1986-01-06 00:00:00
abstract::We prepared an alpha-bungarotoxin-specific monoclonal antibody that neutralizes the biological activity of the toxin in vivo. The antigenic determinant combining specifically with this antibody was determined on the basis of cross-reaction experiments using three other long neurotoxins and peptide fragments of alpha-b...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81018-x
更新日期:1989-08-28 00:00:00
abstract::The Ras homology (Rho) guanine nucleotide exchange factor p115-RhoGEF couples the alpha(13) heterotrimeric guanine nucleotide binding protein (G protein) subunit to Rho GTPase. Alpha(13) binds to a regulator of G protein signaling (RGS) domain in p115-RhoGEF, but the mechanism of alpha(13) activation of p115-RhoGEF is...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00267-9
更新日期:2003-04-10 00:00:00
abstract::The 108-residue protein E. coli thioredoxin has been uniformly enriched to 50% with deuterium at all carbon-bound hydrogen positions. Isotropic mixing (i.e. TOCSY) experiments have been conducted for both the deuterated and natural-abundance samples. Using a 54 ms mixing time correlation peaks can be seen for all four...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)80452-6
更新日期:1988-06-20 00:00:00
abstract::Irreversible conversion of the purified zinc-containing 7Fe ferredoxin from the thermoacidophilic archaeon Sulfolobus sp. strain 7 was found to occur under aerobic conditions at pH 5.0 and at 4 degrees C. This process accompanied a substantial increase of the electron paramagnetic resonance signal attributed to a [3Fe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01286-6
更新日期:1997-11-10 00:00:00
abstract::The folding of HIV gp41 into a 6-helix bundle drives virus-cell membrane fusion. To examine the structural relationship between the 6-helix bundle core domain and other regions of gp41, we expressed in Escherichia coli, the entire ectodomain of HIV-2(ST) gp41 as a soluble, trimeric maltose-binding protein (MBP)/gp41 c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2004.04.054
更新日期:2004-06-04 00:00:00
abstract::In the ameboid eukaryote Dictyostelium discoideum, chemotactic stimulation by cAMP induces an increase of intracellular cGMP and subsequently the phosphorylation of myosin heavy chain II. Resistance to high osmotic stress also requires transient increases of intracellular cGMP and phosphorylation of myosin heavy chain...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00183-5
更新日期:1998-03-13 00:00:00
abstract::We have previously described a partial cDNA sequence encoding a RhoGAP protein, GAP25 that is homologous to the recently reported ArhGAP9 and ArhGAP12. We now describe a related new member ArhGAP15 that shares a number of domain similarities, including a pleckstrin homology (PH) domain, a RhoGAP domain and a novel mot...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00213-8
更新日期:2003-03-27 00:00:00
abstract::The primary structure of 20 beta-hydroxysteroid dehydrogenase from Streptomyces hydrogenans was determined after FPLC purification of a commercial preparation. Peptides obtained from different proteolytic cleavages were purified by reverse phase HPLC. The 255-residue structure deduced was found to be distantly homolog...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81504-h
更新日期:1990-06-18 00:00:00
abstract::MicroRNA (miRNA) play a pivotal role in the development of liver fibrosis. However, the functions of miRNA in hepatitis C virus (HCV)-related liver fibrosis remain unclear. In this study, we systematically analyzed the microarray data of the serum miRNA in patients with HCV-induced hepatic fibrosis. Among 41 dysregula...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12309
更新日期:2016-08-01 00:00:00
abstract::The binding of folate to Lactobacillus casei dihydrofolate reductase in the presence and absence of NADP+ has been studied by 15N NMR, using [5-15N]folate. In the presence of NADP+, three separate signals were observed for the single 15N atom, in agreement with our earlier evidence from 1H and 13C NMR for multiple con...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)81252-8
更新日期:1987-06-08 00:00:00
abstract::Among DNA repair pathways, nucleotide excision repair (NER) is able to recognize and process a wide variety of DNA lesions. The NER mechanism can be summarized in two stages: incision/excision of the lesion and DNA repair synthesis. Here, we have assessed the repair synthesis activity of protein extracts from differen...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01077-6
更新日期:1997-09-15 00:00:00
abstract::We have used HPLC techniques to investigate the effects of gp120 upon inositol phosphate turnover in Jurkat E6-1 CD4+ T-cells, to pursue previous reports that this viral coat protein: (a) inhibits receptor-activated inositol phosphate release; (b) stimulates basal inositol phosphate release; (c) inhibits inositol poly...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00880-6
更新日期:1997-08-11 00:00:00
abstract::p38, which has been suggested to be a scaffold protein for the assembly of a macromolecular tRNA synthetase complex, contains a leucine zipper-like motif. To understand the importance of the leucine zipper-like motif of p38 (p38LZ) in macromolecular assembly, the p38LZ solution structure was investigated by circular d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00362-4
更新日期:2003-05-08 00:00:00
abstract::Regulatory divergence is likely a major driving force in evolution. Comparative genomics is being increasingly used to infer the evolution of gene regulation. Ascomycota fungi are uniquely suited among eukaryotes for regulatory evolution studies, due to broad phylogenetic scope, many sequenced genomes, and tractabilit...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2009.11.032
更新日期:2009-12-17 00:00:00
abstract::We have found that SV40 infection of CV1 cells induces the synthesis of a 72 kDa protein that upon molecular cloning was shown to be the product of the hsc70 gene. The above gene product was found to be mainly virus inducible, in contrast to the hsp70 gene product which was mainly heat inducible. The two genes were fo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01210-5
更新日期:1994-12-05 00:00:00