Functional effects of cytoskeletal components on the lateral movement of individual BKCa channels expressed in live COS-7 cell membrane.

abstract：:We have engineered a genomic HDV ribozyme to construct several trans-acting ribozymes for use in trans to cleave target RNAs. Among the 10 different combinations attempted, only HDV88-Trans had cleavage activity on the 13-nucleotide substrate, R13, in vitro. To improve the cleavage efficiency, at least in vitro, of th...

journal_title：FEBS letters

authors： Kawakami J,Yuda K,Suh YA,Kumar PK,Nishikawa F,Maeda H,Taira K,Ohtsuka E,Nishikawa S

更新日期：1996-09-30 00:00:00

abstract：:Sensitivity of mammalian cells to the bacterial toxin aerolysin is due to the presence at their surface of glycosylphosphatidyl inositol (GPI)-anchored proteins which act as receptors. Using a panel of mutants that are affected in the GPI biosynthetic pathway and Trypanosoma brucei variant surface glycoproteins, we sh...

journal_title：FEBS letters

authors： Abrami L,Velluz MC,Hong Y,Ohishi K,Mehlert A,Ferguson M,Kinoshita T,Gisou van der Goot F

更新日期：2002-02-13 00:00:00

abstract：:We report results from automated docking and microscopic molecular dynamics simulations of the tetraethylammonium (TEA) complexes with KcsA. Binding modes and energies for TEA binding at the external and internal sides of the channel pore are examined utilising the linear interaction energy method. Effects of the chan...

journal_title：FEBS letters

authors： Luzhkov VB,Aqvist J

更新日期：2001-04-27 00:00:00

abstract：:Choroidal melanoma (CM) remains the most prevalent form of intraocular malignancy, and the prognosis of affected patients is poor. While the E3 ubiquitin ligase TRAF-interacting protein (TRIP) is known to play key regulatory roles in multiple diseases, its relevance in CM remains uncertain. In the present study, we fo...

journal_title：FEBS letters

authors： Wei C,Zhao X,Wang L,Zhang H

更新日期：2020-10-01 00:00:00

abstract：:Within 6 h, shear stress upregulated intercellular adhesion molecule-1 (ICAM-1) (two- to four-fold, P<0.001) and induced matrix metalloproteinase-2 (MMP-2) in cultured human saphenous vein endothelial cells. By 8 h endothelial ICAM-1 levels returned to baseline, with concomitant increase in soluble ICAM-1 (sICAM-1) (P...

journal_title：FEBS letters

authors： Sultan S,Gosling M,Nagase H,Powell JT

更新日期：2004-04-23 00:00:00

abstract：:The largest mammalian enzyme family is the kinases. Kinases and other nucleotide-binding proteins are key regulators of signal transduction pathways and the mutation or overexpression of these proteins is often the difference between health and disease. As a result, a massive research effort has focused on understandi...

journal_title：FEBS letters

authors： Rosenblum JS,Nomanbhoy TK,Kozarich JW

更新日期：2013-06-27 00:00:00

abstract：:Internalization of membrane proteins has been studied for more than three decades without solving all the underlying mechanisms. Our knowledge of the clathrin-coated endocytosis is sufficient to understand the basic principles. However, more detailed insight is required to recognize why different proteins enter clathr...

journal_title：FEBS letters

authors： Strous GJ,Gent J

更新日期：2002-10-02 00:00:00

abstract：:Replication protein A (RP-A) was isolated from tobacco suspension cells and purified to near homogeneity by a procedure involving isolation of protoplasts, preparation of nuclei, nuclear lysis, binding to a column of single-stranded (ss) DNA cellulose and elution at different salt concentrations. The purified protein ...

journal_title：FEBS letters

authors： Garcia-Maya MM,Buck KW

更新日期：1997-08-11 00:00:00

abstract：:A high-throughput cell-free protein synthesis method has been described. The methodology is based on a bilayer diffusion system that enables the continuous supply of substrates, together with the continuous removal of small byproducts, through a phase between the translation mixture and substrate mixture. With the use...

journal_title：FEBS letters

authors： Sawasaki T,Hasegawa Y,Tsuchimochi M,Kamura N,Ogasawara T,Kuroita T,Endo Y

更新日期：2002-03-06 00:00:00

abstract：:Chicken muscular dystrophy with abnormal muscle (AM) has been studied for more than 50 years, but the gene responsible for it remains unclear. Our previous studies narrowed down the AM candidate region to approximately 1Mbp of chicken chromosome 2q containing seven genes. In this study, we performed sequence compariso...

journal_title：FEBS letters

authors： Matsumoto H,Maruse H,Inaba Y,Yoshizawa K,Sasazaki S,Fujiwara A,Nishibori M,Nakamura A,Takeda S,Ichihara N,Kikuchi T,Mukai F,Mannen H

更新日期：2008-06-25 00:00:00

abstract：:Leucine-rich repeats (LRRs) are 22-28 amino acid-long sequence motifs found in a family of cytoplasmic, membrane and extracellular proteins. There is evidence that LRRs function in signal transduction, cellular adhesion and protein-protein interactions. Here we report unusual properties of a synthetic LRR peptide deri...

journal_title：FEBS letters

authors： Gay NJ,Packman LC,Weldon MA,Barna JC

更新日期：1991-10-07 00:00:00

abstract：:The accumulation of amyloid-beta (Aβ) and tau aggregates is a pathological hallmark of Alzheimer's disease. Both polypeptides form fibrillar deposits, but several lines of evidence indicate that Aβ and tau form toxic oligomeric aggregation intermediates. Depleting such structures could thus be a powerful therapeutic s...

journal_title：FEBS letters

authors： Wobst HJ,Sharma A,Diamond MI,Wanker EE,Bieschke J

更新日期：2015-01-02 00:00:00

abstract：:Nuclear proteins are actively and posttranslationally transported across the nuclear envelope. This transport is a highly selective process that can be divided into two steps, receptor-binding followed by translocation through the nuclear envelope. Receptor-binding is mediated by nuclear localization signals that have...

journal_title：FEBS letters

authors： Wagner P,Kunz J,Koller A,Hall MN

更新日期：1990-11-26 00:00:00

abstract：:The CLN3 gene encodes an integral membrane protein of unknown function. Mutations in CLN3 can cause juvenile neuronal ceroid lipofuscinosis, or Batten disease, an inherited neurodegenerative lysosomal storage disease affecting children. Here, we report a topological study of the CLN3 protein using bioinformatic approa...

journal_title：FEBS letters

authors： Nugent T,Mole SE,Jones DT

更新日期：2008-04-02 00:00:00

abstract：:Arginine kinase catalyzes the reversible transphosphorylation between adenosine diphosphate (ADP) and phosphoarginine, which is involved in temporal and spatial adenosine triphosphate (ATP) buffering. Here we demonstrate that the homologous overexpression of the Trypanosoma cruzi arginine kinase improves the ability o...

journal_title：FEBS letters

authors： Pereira CA,Alonso GD,Ivaldi S,Silber AM,Alves MJ,Torres HN,Flawiá MM

更新日期：2003-11-06 00:00:00

abstract：:We report that the rat beta 3-adrenergic receptor (beta 3-AR) gene has an intron. The intron starts with an in-frame stop codon with the result that unspliced transcripts will encode a C-terminal truncated protein. The reported protein sequences of mouse and human beta 3-AR were both deduced from genomic DNA sequences...

journal_title：FEBS letters

authors： Bensaid M,Kaghad M,Rodriguez M,Le Fur G,Caput D

更新日期：1993-03-08 00:00:00

abstract：:By use of the signal sequence trap method, we isolated a cDNA encoding a novel aspartic protease-like protein from the mouse kidney, and termed it 'kidney-derived aspartic protease-like protein (KAP).' The protein, a 419-amino-acid polypeptide with a 16-amino-acid signal sequence, had 47% identity with mouse cathepsin...

journal_title：FEBS letters

authors： Mori K,Ogawa Y,Tamura N,Ebihara K,Aoki T,Muro S,Ozaki S,Tanaka I,Tashiro K,Nakao K

更新日期：1997-01-20 00:00:00

abstract：:Three proteinase inhibitor genes have been identified in the rapeseed (Brassica napus) genome. They are highly homologous to other genes of the mustard inhibitor (MSI) family of proteinase inhibitors characteristic of Cruciferae. In germinating seeds, only the transcript of one gene, coding for a trypsin inhibitor, is...

journal_title：FEBS letters

authors： De Leo F,Volpicella M,Sciancalepore M,Gallerani R,Ceci LR

更新日期：2006-02-06 00:00:00

abstract：:A new mutant alpha-1-antichymotrypsin (variant ACT) was found by direct sequencing and PCR-single strand conformation polymorphism (PCR-SSCP). This variant ACT was a point mutation of exon V of ACT, with the substitution of Met by Val. Four out of six individuals with this variant ACT had occlusive-cerebrovascular dis...

journal_title：FEBS letters

authors： Tsuda M,Sei Y,Yamamura M,Yamamoto M,Shinohara Y

更新日期：1992-06-08 00:00:00

abstract：:A Ca2+ indicator arsenazo III was used to demonstrate calcium uptake activity of symbiosomes and the peribacteroid membrane (PBM) vesicles isolated from broad bean root nodules and placed in the medium containing ATP and Mg2+ ions. This process was shown to be rapidly stopped by vanadate, completely reversed in the pr...

journal_title：FEBS letters

authors： Andreev IM,Dubrovo PN,Krylova VV,Izmailov SF

更新日期：1999-03-19 00:00:00

abstract：:Phospholipase D (PLD) has been associated with necrosis. However, it is not clear whether PLD plays a causative role in this cellular process. We investigated the role of PLD in oxidative stress-induced necrosis of vascular smooth muscle cells (VSMCs). Pervanadate (hydrogen peroxide plus orthovanadate) but not hydroge...

journal_title：FEBS letters

authors： Shin EY,Min DS,Shin JC,Shin KS,Hyun MS,Ha KS,Kim HS,Ahn HY,Kim EG

更新日期：2001-11-16 00:00:00

abstract：:We present a rat model in which continuous supply of hepatocyte growth factor (HGF) prevents liver injury induced by carbon tetrachloride (CCl4) and E. coli 011:B4 lipopolysaccharide (LPS). Rat fibroblasts genetically modified to secrete rat HGF were implanted in syngenic rat spleen 7 days before administration of the...

journal_title：FEBS letters

authors： Kaido T,Yamaoka S,Seto S,Funaki N,Kasamatsu T,Tanaka J,Nakamura T,Imamura M

更新日期：1997-07-14 00:00:00

abstract：:Rat glutathione transferase 8-8 is one of the less abundant cytosolic glutathione transferases, accounting for approx. 1% of the total activity with 1-chloro-2,4-dinitrobenzene in liver. The enzyme is eluted at pH 6.3 upon chromatofocusing and has so far been identified in liver, kidney, lung and testis. Characteristi...

journal_title：FEBS letters

authors： Jensson H,Guthenberg C,Alin P,Mannervik B

更新日期：1986-07-28 00:00:00

abstract：:We studied the molecular binding specificity of two rabbit polyclonal sera generated against phenolic glycolipid antigens namely PheG1 B and PheG1 B-3 from Mycobacterium bovis BCG. PheG1 B is the well-known mycoside B (2-O-Me-alpha-L-Rhap 1----aglycone), while PheG1 B-3 is a recently found glycolipid (alpha-L-Rhap-(1-...

journal_title：FEBS letters

authors： Vercellone A,Riviere M,Fournié JJ,Puzo G

更新日期：1992-05-25 00:00:00

abstract：:Prokaryotic aminoacylated-transfer RNAs often need to be efficiently segregated between translation and other cellular biosynthetic pathways. Many clinically relevant bacteria, including Streptococcus pneumoniae, Staphylococcus aureus, Enterococcus faecalis and Pseudomonas aeruginosa direct some aminoacylated-tRNA spe...

journal_title：FEBS letters

authors： Shepherd J,Ibba M

更新日期：2013-09-17 00:00:00

abstract：:We have reported that histone deacetylase (HDAC) inhibitors activate a member of the INK4 family, the p19INK4d gene, causing G1 phase arrest. We report here that HDAC inhibitor, Trichostatin A, activates another member of the INK4 family, the p18INK4c gene, through its promoter in Jurkat cells. Interestingly, the acti...

journal_title：FEBS letters

authors： Yokota T,Matsuzaki Y,Sakai T

更新日期：2004-09-10 00:00:00

abstract：:Rho GTPases participate in various important signaling pathways and have been implicated in myogenic differentiation. Here the first evidence is provided that in C2C12 myoblasts sphingosine 1-phosphate (SPP) rapidly and transiently induced membrane association of Rho A in a pertussis toxin-insensitive manner. The bioa...

journal_title：FEBS letters

authors： Meacci E,Donati C,Cencetti F,Romiti E,Bruni P

更新日期：2000-09-29 00:00:00

abstract：:Hematopoietic progenitors have been shown to retain plasticity and switch lineages by appropriate stimuli. However, mature blood cells hardly showed such differentiation plasticity. In this paper, we tried to reprogram mature B cells into erythroid lineage by expressing various hematopoietic transcription factors. Amo...

journal_title：FEBS letters

authors： Sadahira K,Fukuchi Y,Kunimono H,Sakurai M,Ikeda Y,Okamoto S,Nakajima H

更新日期：2012-10-19 00:00:00

abstract：:Organelles have been studied traditionally as single units, but a novel concept is now emerging: each organelle has distinct functional zones that regulate specific functions. The Golgi apparatus seems to have various zones, including zones for: glycosylphosphatidylinositol-anchored proteins; proteoglycan, mucin and l...

journal_title：FEBS letters

authors： Sasaki K,Yoshida H

更新日期：2019-09-01 00:00:00

abstract：:We have investigated the availability of zinc in the periplasmic space of Escherichia coli using a mutant Cu,Zn superoxide dismutase whose dimerization is triggered by zinc binding. This mutant enzyme accumulates in the monomeric form when wild type cells are grown in minimal medium, but assembles in the dimeric form ...

journal_title：FEBS letters

authors： Berducci G,Mazzetti AP,Rotilio G,Battistoni A

更新日期：2004-07-02 00:00:00