Abstract:
:We report that the rat beta 3-adrenergic receptor (beta 3-AR) gene has an intron. The intron starts with an in-frame stop codon with the result that unspliced transcripts will encode a C-terminal truncated protein. The reported protein sequences of mouse and human beta 3-AR were both deduced from genomic DNA sequences. Given the heterogeneity at the C-termini of the otherwise highly similar rat, mouse and human sequences, we discuss the intriguing possibility that the beta 3-AR gene of the latter two species also contain an intron near the extremity of the open reading frame. A beta-adrenergic receptor (beta-AR) cDNA we have cloned from rat colonic tissue which has a sequence essentially identical to that previously reported for the rat adipose beta 3-AR cDNA [(1991) J. Chem. 266, 24053], encodes the spliced version of the beta 3-AR.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Bensaid M,Kaghad M,Rodriguez M,Le Fur G,Caput Ddoi
10.1016/0014-5793(93)80516-wsubject
Has Abstractpub_date
1993-03-08 00:00:00pages
223-6issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(93)80516-Wjournal_volume
318pub_type
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