Coupled rotation within single F0F1 enzyme complexes during ATP synthesis or hydrolysis.

abstract：:Different bioinformatics methods illuminate different aspects of protein function, from specific catalytic activities to broad functional categories. Here, a triple-pronged approach to predict function for a domain of unknown function, DUF2086, is applied. Distant homology to characterised enzymes and conservation of ...

journal_title：FEBS letters

authors： Mello LV,Rigden DJ

更新日期：2012-11-02 00:00:00

abstract：:The uncoupler-induced inactivation of H+-ATPase in hepatoma 22a and mouse liver mitochondria has been studied. The dependence of this process on delta microH, and pH and ATP was established. The inactivated ATPase could be reactivated at alkaline pH values in the absence of ATP. These data indicate that the inactivati...

journal_title：FEBS letters

authors： Chernyak BV,Dukhovich VF,Khodjaev EYu

更新日期：1987-05-11 00:00:00

abstract：:AtCYP20-2 is one of 16 immunophilins in thylakoid lumen. The presence of the isomerase domain in AtCYP20-2, an enrichment of AtCYP20-2 in the stroma membranes and it's co-migration with NAD(P)H dehydrogenase (NDH) in native gels provide evidence that AtCYP20-2 is an auxiliary protein of NDH. When different NDH mutants...

journal_title：FEBS letters

authors： Sirpiö S,Holmström M,Battchikova N,Aro EM

更新日期：2009-07-21 00:00:00

abstract：:A cDNA clone encoding cytochrome P-450(11)beta of rat adrenal has been cloned and sequenced using a bovine P-450(11)beta cDNA insert (pcP-450(11 beta)-2; (1987) J. Biochem. 102, 559-568) as a probe. The nucleotide sequence contains an open reading frame sufficient to encode the entire amino acid sequence of a P-450(11...

journal_title：FEBS letters

authors： Nonaka Y,Matsukawa N,Morohashi,Omura T,Ogihara T,Teraoka H,Okamoto M

更新日期：1989-09-11 00:00:00

abstract：:Ca2+ release from sarcoplasmic reticulum membranes, activated by alkaline pH occurs only when EGTA is present in the release medium. Addition of very low concentrations of Ca2+ to the medium inhibits Ca2+ release. The concentration of free Ca2+ required for 50% inhibition ranges from between 5 and 20 nM in different e...

journal_title：FEBS letters

authors： Argaman A,Shoshan-Barmatz V

更新日期：1989-01-16 00:00:00

abstract：:A Synechococcus PCC7942 mutant in which the psbO gene was inactivated by insertion of a chloramphenicol interposon and which did not contain any detectable manganese stabilizing protein in immunoblot experiments, was constructed. Such a Synechococcus mutant was able to grow under photoautotrophic conditions. Isolated ...

journal_title：FEBS letters

authors： Bockholt R,Masepohl B,Pistorius EK

更新日期：1991-12-02 00:00:00

abstract：:It was found for RNases of different specificities that changes in the free energy for substrate-enzyme binding induced by variations in the nucleotide base structure are accompanied by proportional changes in kcat/Km. This was considered to be a consequence of the strain in the enzyme-substrate complex. ...

journal_title：FEBS letters

authors： Yakovlev GI,Bocharov AL,Moiseyev GP

更新日期：1984-10-01 00:00:00

abstract：:Targeting gene therapy vectors to abundant receptors on airway epithelia may allow a significant enhancement of gene delivery and thereby be of particular importance for the gene therapy of cystic fibrosis. Alpha9beta1-integrins are highly expressed throughout the human airway epithelia in vivo, irrespective of any pa...

journal_title：FEBS letters

authors： Schneider H,Harbottle RP,Yokosaki Y,Kunde J,Sheppard D,Coutelle C

更新日期：1998-06-16 00:00:00

abstract：:Aminopeptidase yscXVI was purified from the yeast Saccharomyces cerevisiae. By SDS-PAGE the enzyme has a molecular weight of 45,000 Da, and in chromatofocusing, elution was observed at pH 6.2. The synthetic substrate cystinyl-4-nitroanilide (Km 22.5 microM, Vmax 12.9 mU/mg) is cleaved most efficiently in the pH range ...

journal_title：FEBS letters

authors： Tisljar U,Wolf DH

更新日期：1993-05-10 00:00:00

abstract：:The replacement of O2 with CO was studied on cobalt-iron hemoglobin hybrids. Both proto- and mesocobalt hemes were used for the reconstitution. In the oxy quaternary conformation no difference is observed between alpha- and beta-subunits when only proto hemes are present in the hybrid (k4 = 30 s-1, k'4/l'4 = 2.5). If ...

journal_title：FEBS letters

authors： Oertle M,Winterhalter KH,Di Iorio EE

更新日期：1983-03-07 00:00:00

abstract：:The present study investigated the mechanism by which eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) inhibit platelet activation induced by thromboxane A2. DHA was found to be more potent than EPA in blocking platelet aggregation induced by the stable thromboxane A2 mimetic, U46619. Furthermore, this inhib...

journal_title：FEBS letters

authors： Swann PG,Venton DL,Le Breton GC

更新日期：1989-01-30 00:00:00

abstract：:Several analogues of diaminopimelic acid (A2pm) were tested as substrates or inhibitors of the meso-diaminopimelate-adding enzyme from Escherichia coli. They included lanthionine derivatives, a phosphonic analogue, heterocyclic compounds, 3-fluoro-A2pm, 4-methylene-A2pm and N-hydroxy-A2pm. The best substrates were, in...

journal_title：FEBS letters

authors： Auger G,van Heijenoort J,Vederas JC,Blanot D

更新日期：1996-08-05 00:00:00

abstract：:Cytosolic Ca2+ concentration and membrane potential were monitored in individual cultured endothelial cells mechanically stimulated with a micropipette attached to the stage of a microscope. Both dimpling and poking of endothelial cells resulted in Ca2+i transients (from 63 +/- 12 to 397 +/- 52 nM, characterized by a ...

journal_title：FEBS letters

authors： Goligorsky MS

更新日期：1988-11-21 00:00:00

abstract：:Human endothelial cells exposed to H2O2 showed reduced CREP DNA binding activity, enhanced HSF activation, and no induction of NFkappaB binding activity. Interestingly, H2O2 was able to induce NFkappaB subunit p65 translocation in the nucleus. In contrast, cells exposed to TNF alpha showed enhanced CREP binding activi...

journal_title：FEBS letters

authors： Jornot L,Petersen H,Junod AF

更新日期：1997-10-27 00:00:00

abstract：:Ascorbic acid uptake was investigated in isolated, plated human neutrophils using high-performance liquid chromatography with coulometric electrochemical detection. Freshly isolated neutrophils contained 1.3 mM ascorbic acid and accumulated significantly greater amounts when physiologic concentrations of the vitamin w...

journal_title：FEBS letters

authors： Washko P,Rotrosen D,Levine M

更新日期：1990-01-15 00:00:00

abstract：:A cDNA clone encoding cholesterol side-chain cleavage cytochrome P450 (P450scc) was isolated from a rainbow trout ovarian follicle cDNA library. The cDNA contains an open reading frame of 1,542 nucleotides encoding a protein of 514 amino acids. The predicted amino acid sequence of trout P450scc shows 48% homology with...

journal_title：FEBS letters

authors： Takahashi M,Tanaka M,Sakai N,Adachi S,Miller WL,Nagahama Y

更新日期：1993-03-15 00:00:00

abstract：:Membrane proteins are usually solubilized in polar solvents by incorporation into micelles. Even for small membrane proteins these mixed micelles have rather large molecular masses, typically beyond 50000 Da. The NMR technique TROSY (transverse relaxation-optimized spectroscopy) has been developed for studies of struc...

journal_title：FEBS letters

authors： Fernández C,Hilty C,Bonjour S,Adeishvili K,Pervushin K,Wüthrich K

更新日期：2001-08-31 00:00:00

abstract：:As part of a search for selective, mechanism-based covalent inhibitors of human pancreatic α-amylase we describe the chemoenzymatic synthesis of the disaccharide analog α-glucosyl epi-cyclophellitol, demonstrate its stoichiometric reaction with human pancreatic α-amylase and evaluate the time dependence of its inhibit...

journal_title：FEBS letters

authors： Caner S,Zhang X,Jiang J,Chen HM,Nguyen NT,Overkleeft H,Brayer GD,Withers SG

更新日期：2016-04-01 00:00:00

abstract：:We report that glycolysis in human quiescent fibroblasts stimulated by serum addition is increased, and that the changes of the metabolic route reflect the activity of the phosphofructokinase. A possible role of fructose-2,6-bisphosphate as a positive modulator of the key enzyme is proposed. ...

journal_title：FEBS letters

authors： Bruni P,Farnararo M,Vasta V,D'Alessandro A

更新日期：1983-08-08 00:00:00

abstract：:Histone acetyltransferase activity was measured in isolated nuclei during the synchronous cell cycle of the myxomycete Physarum polycephalum. Nuclei were incubated with [14C]acetyl-coenzyme A and an excess of exogenous calf thymus histones. The activity is periodic during the cell cycle; it rises during the S-phase to...

journal_title：FEBS letters

authors： Golderer G,Loidl P,Gröbner P

更新日期：1987-10-05 00:00:00

abstract：:Modulation of intracellular guanosine 3',5'-bispyrophosphate ((p)ppGpp) level, the effector of the stringent response, is crucial for survival as well as optimal growth of prokaryotes and, thus, for bacterial pathogenesis and dormancy. In Mycobacterium tuberculosis (Mtb), (p)ppGpp synthesis and degradation are carried...

journal_title：FEBS letters

authors： Singal B,Balakrishna AM,Nartey W,Manimekalai MSS,Jeyakanthan J,Grüber G

更新日期：2017-08-01 00:00:00

abstract：:We previously identified and purified a nucleolar phosphoprotein, nucleophosmin/B23, as a stimulatory factor for replication from the adenovirus chromatin. We show here that nucleophosmin/B23 functions as a histone chaperone protein such as nucleoplasmin, TAF-I, and NAP-I. Nucleophosmin/B23 was shown to bind to histon...

journal_title：FEBS letters

authors： Okuwaki M,Matsumoto K,Tsujimoto M,Nagata K

更新日期：2001-10-12 00:00:00

abstract：:The X-ray structure of bovine beta-lactoglobulin with the ligand 12-bromododecanoic acid as a model for fatty acids has been determined at a resolution of 2.23 A in the trigonal lattice Z form. The ligand binds inside the calyx, resolving a long-standing controversy as to where fatty-acid like ligands bind. The carbox...

journal_title：FEBS letters

authors： Qin BY,Creamer LK,Baker EN,Jameson GB

更新日期：1998-11-06 00:00:00

abstract：:Ribosomes are ribozymes exerting substrate positioning and promoting substrate-mediated catalysis. Peptide-bonds are formed within a symmetrical region, thus suggesting that ribosomes evolved by gene-fusion. Remote interactions dominate substrate positioning at stereochemistry suitable for peptide-bond formation and e...

journal_title：FEBS letters

authors： Baram D,Yonath A

更新日期：2005-02-07 00:00:00

abstract：:The CLN3 gene encodes an integral membrane protein of unknown function. Mutations in CLN3 can cause juvenile neuronal ceroid lipofuscinosis, or Batten disease, an inherited neurodegenerative lysosomal storage disease affecting children. Here, we report a topological study of the CLN3 protein using bioinformatic approa...

journal_title：FEBS letters

authors： Nugent T,Mole SE,Jones DT

更新日期：2008-04-02 00:00:00

abstract：:The NK-lysin derived peptide NK-2 is a potent antibacterial, but non-toxic to a human keratinocyte cell line and of low hemolytic activity. Its target selectivity is based upon a strong binding preference to membranes containing anionic phospholipids, which are normally not found on the surface of human cells. Here, w...

journal_title：FEBS letters

authors： Schröder-Borm H,Bakalova R,Andrä J

更新日期：2005-11-07 00:00:00

abstract：:The tailspike protein (TSP) of bacteriophage P22 is a homotrimeric multifunctional protein responsible for cell attachment and hydrolysis of the Salmonella typhimurium host cell receptor. Despite the folding of TSP involves the formation of thermolabile intermediates, the mature protein is extremely resistant to heat ...

journal_title：FEBS letters

authors： Carbonell X,Villaverde A

更新日期：1998-08-07 00:00:00

abstract：:The possibility that the mutant mouse wasted (wst/wst) may serve as an animal model for studies of severe combined immunodeficiency disease (SCID) and the role of adenosine deaminase (ADA, EC 3.5.4.4) in adenosine metabolism were investigated. The specific activity of ADA in wst/wst compared with control mice was sign...

journal_title：FEBS letters

authors： Geiger JD,Nagy JI

更新日期：1986-11-24 00:00:00

abstract：:When acquiring internal membranes and vesicular transport, eukaryotic cells started to synthesize sphingolipids and sterols. The physical differences between these and the glycerophospholipids must have enabled the cells to segregate lipids in the membrane plane. Localizing this event to the Golgi then allowed them to...

journal_title：FEBS letters

authors： van Meer G,Hoetzl S

更新日期：2010-05-03 00:00:00

abstract：:Metal-binding peptides in proteolytic digest maps have been identified by matrix-assisted UV laser desorption time-of-flight mass spectrometry (LDTOF-MS). The plasma and milk metal transport protein chosen to demonstrate this process, histidine-rich glycoprotein (HRG), was purified and then digested with trypsin; the ...

journal_title：FEBS letters

authors： Hutchens TW,Nelson RW,Yip TT

更新日期：1992-01-13 00:00:00