Applied jasmonates accumulate extracellularly in tomato, but intracellularly in barley.

abstract：:The rate of formation of diferric-transferrin has been studied using various combinations of Fe(II), Tf, Cp and h serum. When the reactants were added in a correct physiological sequence, ceruloplasmin and diluted human serum showed the fastest rate of saturation of transferrin. ...

journal_title：FEBS letters

authors： Chidambaram MV,Barnes G,Frieden E

更新日期：1983-08-08 00:00:00

abstract：:The crystal structure of Arthromyces ramosus peroxidase (ARP) in complex with benzhydroxamic acid (BHA) as determined by X-ray analysis at 1.6 A shows unambiguously how BHA binds to ARP. BHA is located in the distal heme pocket. Its functional groups are held by three hydrogen bonds to His56N(epsilon), Arg52N(epsilon)...

journal_title：FEBS letters

authors： Itakura H,Oda Y,Fukuyama K

更新日期：1997-07-21 00:00:00

abstract：:Small interfering (si)RNAs isolated from Brassica juncea leaves infected by Turnip mosaic virus (TuMV) were characterized by cloning and sequencing. The TuMV siRNA population was dominated by 21 and 22-nt long species originated mainly from the same siRNA hotspots, indicating operational similarity between the plant D...

journal_title：FEBS letters

authors： Ho T,Wang H,Pallett D,Dalmay T

更新日期：2007-07-10 00:00:00

abstract：:Polyhydroxyalkanoate (PHA) synthases catalyze the polymerization reaction of the acyl moiety of hydroxyacyl-coenzyme A into polyester. The catalytic subunit PhaC of PHA synthase has the PhaC box sequence at the active site that is typically described as G-X-C-X-G-G (X is an arbitrary amino acid), and cysteine is an ac...

journal_title：FEBS letters

authors： Nambu Y,Ishii-Hyakutake M,Harada K,Mizuno S,Tsuge T

更新日期：2020-02-01 00:00:00

abstract：:A double-blinded, placebo-controlled cross-over trial was carried out with 27 hypercholesterolemic men with coronary heart disease. During the 6-week treatment period lovastatin (60 mg/day) decreased fasting serum LDL cholesterol by 45%, LDL phosphorus by 38% and apoB by 33%. Ubiquinol content diminished by 13% as mea...

journal_title：FEBS letters

authors： Palomäki A,Malminiemi K,Metsä-Ketelä T

更新日期：1997-06-30 00:00:00

abstract：:Exposure of HeLa and HL60 cells to sodium arsenite or cadmium chloride led to marked increases in cellular heme oxygenase activity. SDS-polyacrylamide gel electrophoresis of [35S]methionine-labeled cellular proteins indicated that these treatments also resulted in the induction of a 32-kDa protein. Immunoblot analysis...

journal_title：FEBS letters

authors： Taketani S,Kohno H,Yoshinaga T,Tokunaga R

更新日期：1989-03-13 00:00:00

abstract：:Redox reactions of tyrosine play key roles in many biological processes, including water oxidation and DNA synthesis. We first review the redox properties of tyrosine (and other phenols) in small molecules and related polypeptides, then report work on (H20)/(Y48)-modified Pseudomonas aeruginosa azurin. The crystal str...

journal_title：FEBS letters

authors： Warren JJ,Winkler JR,Gray HB

更新日期：2012-03-09 00:00:00

abstract：:The conformational behavior of cyclic peptides of the amino acid sequence Cys-Phe/Ala-Pro-Ala-Cys has been investigated through the combined use of molecular simulation methods and NMR experiments to find models for beta-VIa turns of proteins. Both oxidized (cyclic) peptides and reduced (linear) forms were investigate...

journal_title：FEBS letters

authors： Weisshoff H,Frost K,Brandt W,Henklein P,Mügge C,Frömmel C

更新日期：1995-09-25 00:00:00

abstract：:The fidelity of tRNA aminoacylation is dependent in part on amino acid editing mechanisms. A hydrolytic activity that clears mischarged tRNAs typically resides in an active site on the tRNA synthetase that is distinct from its synthetic aminoacylation active site. A second pre-transfer editing pathway that hydrolyzes ...

journal_title：FEBS letters

authors： Martinis SA,Boniecki MT

更新日期：2010-01-21 00:00:00

abstract：:Farnesoid X receptor (FXR) is highly expressed in liver and intestine where it controls bile acid (BA), lipid and glucose homeostasis. Here we show that FXR is expressed and functional, as assessed by target gene expression analysis, in human islets and beta-cell lines. FXR is predominantly cytosolic-localized in the ...

journal_title：FEBS letters

authors： Popescu IR,Helleboid-Chapman A,Lucas A,Vandewalle B,Dumont J,Bouchaert E,Derudas B,Kerr-Conte J,Caron S,Pattou F,Staels B

更新日期：2010-07-02 00:00:00

abstract：:The crystal structure of metagenome-derived LC9-RNase H1 was determined. The structure-based mutational analyses indicated that the active site motif of LC9-RNase H1 is altered from DEDD to DEDN. In this motif, the location of the second glutamate residue is moved from αA-helix to β1-strand immediately next to the fir...

journal_title：FEBS letters

authors： Nguyen TN,You DJ,Kanaya E,Koga Y,Kanaya S

更新日期：2013-05-02 00:00:00

abstract：:Insolubility in non-ionic detergents such as Triton X-100 is a widely used biochemical criterion for characterization of membrane domains. We report here a novel green fluorescent protein fluorescence-based approach to directly determine detergent insolubility of specific membrane proteins. We have applied this method...

journal_title：FEBS letters

authors： Kalipatnapu S,Chattopadhyay A

更新日期：2004-10-22 00:00:00

abstract：:Tumor necrosis factor TNF can trigger increases in membrane conductance of mammalian cells in a receptor-independent manner via its lectin-like domain. A lectin-deficient TNF mutant, lacking this activity, was able to bind to artificial liposomes in a pH-dependent manner, but not to insert into the bilayer, just like ...

journal_title：FEBS letters

authors： van der Goot FG,Pugin J,Hribar M,Fransen L,Dunant Y,De Baetselier P,Bloc A,Lucas R

更新日期：1999-10-22 00:00:00

abstract：:An Mr 21 000 polypeptide, designated APPG, has been purified by reverse-phase, high-performance liquid chromatography (RP-HPLC), from acid extracts of porcine anterior pituitary glands. This acidic protein possesses an isoelectric point of 4.9. Amino acid analysis shows that it is not a glycoprotein and estimates it t...

journal_title：FEBS letters

authors： Hsi KL,Seidah NG,De Serres G,Chrétien M

更新日期：1982-10-18 00:00:00

abstract：:ISG20 is an interferon-induced antiviral exoribonuclease that acts on single-stranded RNA and also has minor activity towards single-stranded DNA. It belongs to the DEDDh group of RNases of the DEDD exonuclease superfamily. We have solved the crystal structure of human ISG20 complexed with two Mn2+ ions and uridine 5'...

journal_title：FEBS letters

authors： Horio T,Murai M,Inoue T,Hamasaki T,Tanaka T,Ohgi T

更新日期：2004-11-05 00:00:00

abstract：:Currently a central question that remains unresolved is the potential importance of phospholipase C (PLC)-gamma1 in cell mitogenesis. In this study, we introduced wild-type PLC-gamma1 plasmid and mutants Y771F and Y783F into CCL-39 fibroblasts and investigated their effect on host cell functions. To our surprise, Y771...

journal_title：FEBS letters

authors： Pei ZD,Williamson JR

更新日期：1998-02-13 00:00:00

abstract：:Treatment of cultured carrot cells with dibutyryl cAMP or forskolin resulted in the appreciable decrease in extracellular K+ concentration. This decrease was found to be transient and the concentration of the ion in the culture medium restored to the original level within few minutes. The cAMP-induced decrease in K+ l...

journal_title：FEBS letters

authors： Kurosaki F

更新日期：1997-05-12 00:00:00

abstract：:The half-lives of mRNAs in yeast vary from about 1 to over 100 min. While mRNA stabilities must strongly influence overall gene expression in this organism, very little is known about how they are determined. Labellings of yeast cells were conducted to investigate whether the 5'-cap structures of yeast mRNAs might inf...

journal_title：FEBS letters

authors： Piper PW,Curran B,Davies W,Hirst K,Seward K

更新日期：1987-08-10 00:00:00

abstract：:The interaction of the P-beta-Cat(19-44) peptide, a 26 amino acid peptide (K(19)AAVSHWQQQSYLDpSGIHpSGATTTAP(44)) that mimics the phosphorylated beta-Catenin antigen, has been studied with its monoclonal antibody BC-22, by transferred nuclear Overhauser effect NMR spectroscopy (TRNOESY) and saturation transfer differen...

journal_title：FEBS letters

authors： Megy S,Bertho G,Gharbi-Benarous J,Baleux F,Benarous R,Girault JP

更新日期：2006-10-02 00:00:00

abstract：:xxR/KxRxxSxx sequences were phosphorylated with high efficiency by both p70 S6 kinase (p70S6K) and MAPKAP kinase-1. The best substrate for MAPKAP kinase-1 (KKKNRTLSVA) was phosphorylated with a Km of 0.17 microM, and the best substrate for p70S6K (KKRNRTLSVA) with a Km of 1.5 microM. The requirement of both enzymes fo...

journal_title：FEBS letters

authors： Leighton IA,Dalby KN,Caudwell FB,Cohen PT,Cohen P

更新日期：1995-11-20 00:00:00

abstract：:When acquiring internal membranes and vesicular transport, eukaryotic cells started to synthesize sphingolipids and sterols. The physical differences between these and the glycerophospholipids must have enabled the cells to segregate lipids in the membrane plane. Localizing this event to the Golgi then allowed them to...

journal_title：FEBS letters

authors： van Meer G,Hoetzl S

更新日期：2010-05-03 00:00:00

abstract：:The degradation of type IX collagen, a minor collagen in cartilage, was examined by treatment with three different types of matrix metalloproteinases (MMPs) purified from the culture medium of rheumatoid synovial cells. Neither MMP-1 (collagenase) nor MMP-2 (so-called 'gelatinase') could digest type IX collagen, but M...

journal_title：FEBS letters

authors： Okada Y,Konomi H,Yada T,Kimata K,Nagase H

更新日期：1989-02-27 00:00:00

abstract：:Single rat hepatocytes microinjected with aequorin show free Ca oscillations when stimulated with Ca(2+)-mobilizing hormones. We show here that an inhibitor of diacylglycerol kinase (R 59 022) and an analogue of native diacylglycerol (diC8) inhibit free Ca oscillations induced by phenylephrine and vasopressin. These r...

journal_title：FEBS letters

authors： Sanchez-Bueno A,Cobbold PH

更新日期：1992-11-02 00:00:00

abstract：:Binding of vitronectin (VN) to Neisseria gonorrhoeae expressing the heparan sulfate proteoglycan (HSPG) specific Opa50 protein was recently shown to trigger bacterial internalization into distinct epithelial cell lines. We have investigated the role of VN-binding integrin receptors and protein kinase C (PKC) in VN-tri...

journal_title：FEBS letters

authors： Dehio M,Gómez-Duarte OG,Dehio C,Meyer TF

更新日期：1998-03-06 00:00:00

abstract：:The ubiquitin-like protein Urm1 from budding yeast and its E1-like activator Uba4 have dual roles in protein urmylation and tRNA thiolation pathways. To study whether these are conserved among eukaryotes, we used gene shuffles to replace the yeast proteins by their human counterparts, hURM1 and hUBA4/MOCS3. As judged ...

journal_title：FEBS letters

authors： Jüdes A,Ebert F,Bär C,Thüring KL,Harrer A,Klassen R,Helm M,Stark MJ,Schaffrath R

更新日期：2015-04-02 00:00:00

abstract：:Sequential reverse transcription and polymerase chain reaction (RT-PCR) of the mRNA were used to investigate the expression of dopamine receptors in human peripheral blood lymphocytes. The RT-PCR products contained three types of sequences, each corresponding to those of the D5 dopamine receptor gene and the two relat...

journal_title：FEBS letters

authors： Takahashi N,Nagai Y,Ueno S,Saeki Y,Yanagihara T

更新日期：1992-12-07 00:00:00

abstract：:Photon correlation spectroscopy demonstrated for the first time that co-purified meningococcal TbpA+B form a complex in solution. This structure bound hTf and the resultant species underwent partial dissociation after exposure to additional hTf or following prolonged incubation. Purified TbpA and TbpB had similar appa...

journal_title：FEBS letters

authors： Boulton IC,Gorringe AR,Carr RJ,Gorinsky B,Joannou CL,Evans RW

更新日期：1997-09-08 00:00:00

abstract：:The activity of S-adenosylmethionine decarboxylase (AdoMetDC) in Crithidia fasciculata was shown to be correlated to the growth of the parasite. An increase in activity was observed during exponential growth. Inhibition of protein synthesis induced an extremely rapid decay of AdoMetDC activity. The half-life of the en...

journal_title：FEBS letters

authors： Nasizadeh S,Persson L

更新日期：2003-10-09 00:00:00

abstract：:Degradation of extracellular matrix proteins is performed by metalloproteinases which are inhibited by tissue inhibitors of metalloproteinases (TIMP). We expressed the murine TIMP-1 protein in E. coli and prepared a polyclonal antiserum against the recombinant protein. Using this antiserum we studied the biosynthesis ...

journal_title：FEBS letters

authors： Roeb E,Graeve L,Müllberg J,Matern S,Rose-John S

更新日期：1994-07-25 00:00:00

abstract：:Thrombin is a potent activator of human glomerular epithelial cells (HGEC). Here we compare short-term and long-term effects of thrombin and thrombin receptor agonist peptide (TRAP) which selectively activates the functional thrombin receptor. TRAP, as thrombin, increases intracellular free Ca2+ concentration and acts...

journal_title：FEBS letters

authors： Zacharias U,He CJ,Nguyen G,Sraer JD,Rondeau E

更新日期：1993-11-15 00:00:00