Abstract:
:Small interfering (si)RNAs isolated from Brassica juncea leaves infected by Turnip mosaic virus (TuMV) were characterized by cloning and sequencing. The TuMV siRNA population was dominated by 21 and 22-nt long species originated mainly from the same siRNA hotspots, indicating operational similarity between the plant Dicer-like (DCL) enzymes. Robust GC bias was observed for TuMV siRNAs versus the virus genome, indicating that DCL was more likely to target GC-rich regions. Furthermore, dicot micro-(mi)RNAs displayed higher GC% than their DCL1 substrate RNAs, implicating that the GC bias may be ancient, therefore may be important for the RNAi technology.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Ho T,Wang H,Pallett D,Dalmay Tdoi
10.1016/j.febslet.2007.06.022subject
Has Abstractpub_date
2007-07-10 00:00:00pages
3267-72issue
17eissn
0014-5793issn
1873-3468pii
S0014-5793(07)00671-0journal_volume
581pub_type
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