Abstract:
:The pathogenicities of influenza viruses and paramyxoviruses have been proposed to be primarily determined by a host cell protease(s) that activates viral infectivity by proteolytic cleavage of the envelope glycoproteins. We recently isolated a trypsin-type endoprotease, named tryptase Clara, from rat bronchial and bronchiolar epithelial Clara cells, which is secreted into the airway lumen and activates Sendai virus and influenza A virus proteolytically. We report here that surfactant in the bronchial fluid inhibited tryptase Clara specifically, having a Ki value of 0.13 microM, and inhibited the proteolytic activations by tryptase Clara in vitro and in organ cultures of rat lung. Intranasal infection of rats with Sendai virus was shown to stimulate secretion of tryptase Clara without changing the amount of surfactant in the bronchial lumen, resulting in a preferable condition for proteolytic viral activation and multiplication.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Kido H,Sakai K,Kishino Y,Tashiro Mdoi
10.1016/0014-5793(93)81549-fsubject
Has Abstractpub_date
1993-05-10 00:00:00pages
115-9issue
2eissn
0014-5793issn
1873-3468pii
0014-5793(93)81549-Fjournal_volume
322pub_type
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