Expression of a p16INK4a-specific ribozyme downmodulates p16INK4a abundance and accelerates cell proliferation.

abstract：:Insulin resistant states are associated with an increase in the beta cell mass and also high levels of circulating insulin. Ultimately the beta cells undergo a failure that leads to diabetes. At this stage, a question arises if those persistent high levels of circulating insulin may contribute to beta cell damage. To ...

journal_title：FEBS letters

authors： Guillen C,Bartolomé A,Nevado C,Benito M

更新日期：2008-11-26 00:00:00

abstract：:The role of Shine-Dalgarno-like sequences in mRNAs from higher plant chloroplasts has not been analyzed experimentally so far. In vitro translation analysis has revealed that the Shine-Dalgarno-like sequence is essential for translation of tobacco chloroplast rps14 mRNA. Two RNA editing sites have been identified in t...

journal_title：FEBS letters

authors： Hirose T,Kusumegi T,Sugiura M

更新日期：1998-07-03 00:00:00

abstract：:A new inositol derivative could be isolated from the Archaeum Pyrococcus woesei and identified as di-myo-inositol-1,1'-phosphate by 1H, 31P NMR spectroscopy, mass spectrometry and thin layer chromatography. In P. woesei, this inositol phosphate represents the dominant counterion of K+ which ranges from 500 to 600 mM. ...

journal_title：FEBS letters

authors： Scholz S,Sonnenbichler J,Schäfer W,Hensel R

更新日期：1992-07-20 00:00:00

abstract：:Apolipoprotein E (apoE) is predominantly synthesized by astrocytes in the brain. In this study, we investigated the role of apoE in astrocyte apoptosis. We demonstrated that apoE protects astrocytes from hypoxia-induced apoptosis in a dose-dependent manner. Glutamate release from astrocytic cultures is significantly l...

journal_title：FEBS letters

authors： Zhou S,Wu H,Zeng C,Xiong X,Tang S,Tang Z,Sun X

更新日期：2013-01-16 00:00:00

abstract：:Cloned mouse delta-subtype opioid receptor (DOR1) was expressed in Xenopus oocytes to study the signal transduction. Opioid delta-agonists evoked a calcium-dependent chloride current in oocytes injected with mRNA derived from DOR1, together with that from the alpha subunit of Gi1. The delta-agonist-induced current was...

journal_title：FEBS letters

authors： Miyamae T,Fukushima N,Misu Y,Ueda H

更新日期：1993-11-01 00:00:00

abstract：:There are two secondary structure models for the eukaryotic selenocysteine (Sec) tRNA(Sec). One model, the 9/4 structure, was experimentally tested and possesses acceptor and T-stems with 9 and 4 bp, respectively [Sturchler et al., 1993; Hubert et al., 1998]. The other one, the 7/5 secondary structure with a bulge in ...

journal_title：FEBS letters

authors： Mizutani T,Goto C

更新日期：2000-01-28 00:00:00

abstract：:The role of p53 as the "guardian of the genome" in differentiated somatic cells, triggering various biological processes, is well established. Recent studies in the stem cell field have highlighted a profound role of p53 in stem cell biology as well. These studies, combined with basic data obtained 20 years ago, provi...

journal_title：FEBS letters

authors： Aloni-Grinstein R,Shetzer Y,Kaufman T,Rotter V

更新日期：2014-08-19 00:00:00

abstract：:Early in the course of pregnancy, at the preimplantation stage, the pig embryo is likely to exert a paracrine effect on the tissue intended to receive it, via the secretion of interferons. Our observations show that trophoblastic interferons induce an increase of some mRNAs in the epithelial cells of the gilt endometr...

journal_title：FEBS letters

authors： Chwetzoff S,d'Andrea S

更新日期：1997-03-24 00:00:00

abstract：:The inhibitor of gamma-glutamyl transpeptidase (gamma-GT) acivicin modulates cellular responses including growth, myeloid maturation and apoptosis. Whether these effects result from the inhibition of gamma-GT enzyme activity remains unclear. We compared the cellular effects of acivicin against a more potent and specif...

journal_title：FEBS letters

authors： Antczak C,Karp DR,London RE,Bauvois B

更新日期：2001-11-16 00:00:00

abstract：:The dgd1 mutant of Arabidopsis thaliana provides us with a powerful tool for revealing the specific role of digalactosyldiacylglycerol (DGDG) in photosynthesis. Blue-native polyacrylamide gel electrophoresis analysis revealed that photosystem I (PSI) subunits are assembled into a PSI complex, and that a PSI subcomplex...

journal_title：FEBS letters

authors： Guo J,Zhang Z,Bi Y,Yang W,Xu Y,Zhang L

更新日期：2005-07-04 00:00:00

abstract：:SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)1H-imi dazole) is widely used as a specific inhibitor of p38 mitogen-activated protein kinase (MAPK). Here, we report that SB203580 activates the serine/threonine kinase Raf-1 in quiescent smooth muscle cells in a dose-dependent fashion. The concentr...

journal_title：FEBS letters

authors： Kalmes A,Deou J,Clowes AW,Daum G

更新日期：1999-02-05 00:00:00

abstract：:Lipopolysaccharides (LPS, endotoxin) stimulate mononuclear cells to release cytokines which initiate endotoxic effects. Interaction of LPS at low concentrations with target cells is CD14-dependent whereas at high LPS concentrations it is CD14-independent. Here, we demonstrate by resonance energy transfer (RET) techniq...

journal_title：FEBS letters

authors： Schromm AB,Brandenburg K,Rietschel ET,Flad HD,Carroll SF,Seydel U

更新日期：1996-12-16 00:00:00

abstract：:(-)-Epigallocatechin-3-O-gallate (EGCG), a polyphenol in green tea, induces apoptosis in acute myeloid leukemia (AML) cells without affecting normal cells. In this study, we observed that cGMP acts as a cell death mediator of the EGCG-induced anti-AML effect through acid sphingomyelinase activation. EGCG activated the...

journal_title：FEBS letters

authors： Kumazoe M,Kim Y,Bae J,Takai M,Murata M,Suemasu Y,Sugihara K,Yamashita S,Tsukamoto S,Huang Y,Nakahara K,Yamada K,Tachibana H

更新日期：2013-09-17 00:00:00

abstract：:Boar semen contains a seminal plasma motility inhibitor (SPMI) that blocks the motility of demembranated-reactivated spermatozoa as well as of intact spermatozoa. In this paper, we describe the primary structure of SPMI, the coding of boar SPMI cDNA gene and its expression in various porcine tissues. Nucleotide sequen...

journal_title：FEBS letters

authors： Iwamoto T,Hiroaki H,Furuichi Y,Wada K,Satoh M,Satoh M,Osada T,Gagnon C

更新日期：1995-07-24 00:00:00

abstract：:Complexes between the urokinase-type plasminogen activator (uPA) and its type-2 inhibitor (PAI-2) are bound by a cell-surface receptor for uPA and rapidly cleaved into two fragments of 70 and 22 kDa. The 70-kDa fragment contains the active site of uPA and PAI-2, while the 22-kDa species was identified as the amino ter...

journal_title：FEBS letters

authors： Ragno P,Montuori N,Vassalli JD,Rossi G

更新日期：1993-06-01 00:00:00

abstract：:Contact inhibition of locomotion (CIL) occurs when a cell ceases moving in the same direction following contact with another cell. Homotypic and heterotypic CIL occur between cells of the same and different types, respectively. Using Abercrombie's confronted explants assay we studied the effect of changing Rac1 or Rho...

journal_title：FEBS letters

authors： Anear E,Parish RW

更新日期：2012-05-07 00:00:00

abstract：:A latent MAP kinase kinase kinase activity previously detected after chromatography of PC12 cell extracts on Mono Q [(1992) FEBS Lett. 314, 461-465] has been identified as B-Raf by immunoblotting and immunoprecipitation experiments and by its specific activation with B-Raf antibodies. B-Raf is phosphorylated after sti...

journal_title：FEBS letters

authors： Traverse S,Cohen P

更新日期：1994-08-15 00:00:00

abstract：:Patients suffering from Refsum disease have a defect in the alpha-oxidation pathway which results in the accumulation of phytanic acid in plasma and tissues. Our previous studies have shown that phytanic acid is also a substrate for the omega-oxidation pathway. With the use of specific inhibitors we now show that memb...

journal_title：FEBS letters

authors： Komen JC,Wanders RJ

更新日期：2006-07-10 00:00:00

abstract：:Side chain-hydroxylated derivatives of cholesterol (OH sterol) inhibiting lymphoblastic transformation bind with high affinity and specificity to a hydroxysterol binding protein (OHSBP) in the cytosol of human lymphocytes. These binding properties of OHSBP suggested some analogies with that of steroid hormone receptor...

journal_title：FEBS letters

authors： Defay R,Astruc M,Beseme F,Descomps B,Crastes de Paulet A

更新日期：1984-08-06 00:00:00

abstract：:A poly(A) tail functions in mRNA turnover and in facilitating translation as a ribonucleoprotein complex with poly(A) binding proteins (PABPs). However, factors that associate with the poly(A) tail other than PABPs have not been described. Using proteomics, we identified candidate proteins that interact to the 3' term...

journal_title：FEBS letters

authors： Aoki K,Adachi S,Homoto M,Kusano H,Koike K,Natsume T

更新日期：2013-07-11 00:00:00

abstract：:WT1 was isolated as a tumor suppressor gene of Wilms tumor. However, high expression of WT1 correlates with poor prognosis in acute leukemia. In addition suppression of WT1 expression by WT1 anti-sense oligonucleotide inhibits proliferation of leukemia cells, suggesting that WT1 is important for their proliferation. T...

journal_title：FEBS letters

authors： Murata Y,Kudoh T,Sugiyama H,Toyoshima K,Akiyama T

更新日期：1997-06-02 00:00:00

abstract：:We analyzed the expression of aquaporins (AQPs) in the small intestine to elucidate their functions, and found that AQP9, which had not previously been detected there, is present in duodenum, jejunum, and ileum. AQP9 is expressed in colon as well, but not in stomach. Also, its expression in these intestinal sections i...

journal_title：FEBS letters

authors： Okada S,Misaka T,Matsumoto I,Watanabe H,Abe K

更新日期：2003-04-10 00:00:00

abstract：:Glycosaminoglycans (GAGs) are complexed with plasma proteins and proteolysis of plasma reduced the protein-GAG ratio about 140-fold. After dialysis, analysis by gradient PAGE revealed heparinase-1-sensitive GAGs, thus suggesting that heparin could be among the plasma GAGs. However, after dialysis most of the plasma GA...

journal_title：FEBS letters

authors： Chevanne M,Caldini R,Manao G,Ruggiero M,Vannucchi S

更新日期：1999-12-10 00:00:00

abstract：:A cDNA clone encoding the sequence of human pancreatic lipase (HPL) was subcloned into the baculovirus transfer vector pVL1392 and used in co-transfection of Spodoptera frugiperda (Sf9) insect cells with wild-type Autographa californica nuclear polyhedrosis virus (AcNPV) DNA. A single recombinant protein (50 kDa) secr...

journal_title：FEBS letters

authors： Thirstrup K,Carrière F,Hjorth S,Rasmussen PB,Wöldike H,Nielsen PF,Thim L

更新日期：1993-07-19 00:00:00

abstract：:Isolated mitotic chromosomes are able to form complexes with phosphatidylcholine liposomes in the presence and absence of Ca2+ ions, in the latter case in the presence of polyamines. Interactions with chromosomes stimulates liposome fusion. The fusion is promoted by condensed and EDTA-decondensed chromosomes. ...

journal_title：FEBS letters

authors： Balakireva LA,Belyaev ND,Budker VG,Kiseleva EV

更新日期：1991-06-24 00:00:00

abstract：:Arabidopsis thaliana contains three genes with high homology to potato p24 which was described as a member of the Whirly family of nuclear transcriptional activators. Computer-based analysis revealed that all Arabidopsis Whirly (Why) proteins contain targeting sequences for either plastids or mitochondria. The functio...

journal_title：FEBS letters

authors： Krause K,Kilbienski I,Mulisch M,Rödiger A,Schäfer A,Krupinska K

更新日期：2005-07-04 00:00:00

abstract：:We previously showed that P4-ATPases, ATP10A/ATP8B1, and ATP11A/ATP11C have flippase activities toward phosphatidylcholine (PC), and aminophospholipids [phosphatidylserine (PS) and phosphatidylethanolamine], respectively. Here, we investigate the effect of PC-specific flippases versus aminophospholipid-specific flippa...

journal_title：FEBS letters

authors： Miyano R,Matsumoto T,Takatsu H,Nakayama K,Shin HW

更新日期：2016-07-01 00:00:00

abstract：:125I-labelled angiotensin II (A II) specifically binds to solubilized receptors extracted from rat isolated glomeruli using CHAPS (3-[3-( cholamidopropyl ) dimethylammonio ]-1-propanesulfonate). The yield of solubilization of the binding sites was 3.3%. Equilibrium was reached after 15-20 min and specific binding repr...

journal_title：FEBS letters

authors： Chansel D,Ardaillou N,Ardaillou R

更新日期：1984-05-21 00:00:00

abstract：:The complete amino acid sequence of the oligomycin sensitivity-conferring protein (OSCP) of beef-heart mitochondria is reported. The protein contains 190 amino acids and has a molecular mass of 20 967. Its structure is characterized by a concentration of charged amino acids in the two terminal segments (N 1-77 and C 1...

journal_title：FEBS letters

authors： Ovchinnikov YA,Modyanov NN,Grinkevich VA,Aldanova NA,Trubetskaya OE,Nazimov IV,Hundal T,Ernster L

更新日期：1984-01-23 00:00:00

abstract：:Uncoupling protein 3 (UCP3) may reduce mitochondrial ROS production, and thereby protect against mitochondrial dysfunction in skeletal muscle. UCP3 has been suggested to specifically fulfill this role under high-fat conditions. Here we show that UCP3 knockout mice indeed have elevated mitochondrial ROS production afte...

journal_title：FEBS letters

authors： Nabben M,Hoeks J,Moonen-Kornips E,van Beurden D,Briedé JJ,Hesselink MK,Glatz JF,Schrauwen P

更新日期：2011-12-15 00:00:00