Abstract:
:A cDNA clone encoding the sequence of human pancreatic lipase (HPL) was subcloned into the baculovirus transfer vector pVL1392 and used in co-transfection of Spodoptera frugiperda (Sf9) insect cells with wild-type Autographa californica nuclear polyhedrosis virus (AcNPV) DNA. A single recombinant protein (50 kDa) secreted by Sf9 cells was detectable in the culture medium 24 h post-infection using both anti-HPL polyclonal antibodies and potentiometric measurements of lipolytic activity. The expression level reached 40 mg/l of enzyme at 6 days. A single cation-exchange chromatography was sufficient to obtain a highly pure recombinant HPL as demonstrated by N-terminal sequencing, amino acid composition and carbohydrate analysis, as well as by mass spectrometry. These analyses revealed the production of mature protein with the correct processing of signal peptide and an homogenous glycosylation pattern. The kinetic properties of recombinant and native HPL were compared. Both enzymes showed similar profiles of interfacial activation, inhibition by bile salts and re-activation by colipase.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Thirstrup K,Carrière F,Hjorth S,Rasmussen PB,Wöldike H,Nielsen PF,Thim Ldoi
10.1016/0014-5793(93)81044-zsubject
Has Abstractpub_date
1993-07-19 00:00:00pages
79-84issue
1eissn
0014-5793issn
1873-3468pii
0014-5793(93)81044-Zjournal_volume
327pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Muscarinic acetylcholine receptors purified from porcine atrium were phosphorylated, depending on the presence of agonists, by a protein kinase partially purified from porcine brain, which had similar properties to the beta-adrenergic receptor kinase. GTP-binding regulatory proteins (Go) had dual effects on the phosph...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80968-o
更新日期:1990-07-30 00:00:00
abstract::In HL-60 cells signal transduction via sphingomyelin hydrolysis (sphingomyelin cycle) is induced by binding of tumor necrosis factor alpha (TNFalpha) to cell surface TNFalpha receptor. We found that IgG immunoglobulins activate sphingomyelin hydrolysis in plasma membrane of HL-60 cells, with kinetics similar to that o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00958-1
更新日期:1996-10-07 00:00:00
abstract::In a previous study [(1987) J. Mol. Biol. 194, 663-673], we isolated ten insertion/deletion mutants (indels) of the maltose binding protein for which the maltose binding constant was only a little or not at all affected. In this paper, we have localized these mutations in the recently solved three-dimensional structur...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/0014-5793(93)81409-s
更新日期:1993-06-28 00:00:00
abstract::The induction of apoptosis by cell cycle regulator molecules under conditions optimal for exponential growth was examined in rat pheochromocytoma PC12 cells by overexpression of cyclins and cyclin-dependent kinases (cdks). By flow cytometry and by immunofluorescence, only cells overexpressing cdk4 or cyclin D1 underwe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)03200-8
更新日期:2001-12-14 00:00:00
abstract::The malaria parasite harbours an indispensable plastid known as the 'apicoplast'. The apicoplast's exact role remains uncertain, but it houses components involved in fatty acid, isoprenoid and haem biosyntheses. These pathways offer opportunities to develop anti-malarials. In the absence of photosynthesis, how apicopl...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.11.097
更新日期:2010-02-05 00:00:00
abstract::Two different clones of Swiss 3T3 cells belonging to the same original cell line have been obtained, one of which was unresponsive to mitogenic stimulation (e.g. insulin-like growth factor-I, bombesin, insulin-like growth factor-I + bombesin), while the other clone showed a very high rate of DNA synthesis under identi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80598-w
更新日期:1991-06-03 00:00:00
abstract::The purpose of this investigation was to study the effect on cap structure for trans-splicing in mammalian cells. The early SV40 Bst/Bam pre-mRNA (cRNA) was synthesized in vitro in both capped (cap-Bst/Bam-cRNA) and non-capped (Bst/Bam-cRNA) versions and microinjected into the nuclei of TC7 cells. Trans-splicing was m...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00956-8
更新日期:1996-09-30 00:00:00
abstract::The glucagon-like peptide 1 (7-37)/(7-36) amide (GLP-1) receptor belongs to a new subclass of seven transmembrane domain, G-protein coupled receptors comprising several receptors for peptide hormones. The receptors of this family share many common motifs including a relatively large N-terminal extracellular domain. Th...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(96)01214-8
更新日期:1996-11-25 00:00:00
abstract::Lipids are essential eukaryotic cellular constituents. Lipid metabolism has a strong impact on cell physiology, and despite good progress in this area, many important basic questions remain unanswered concerning the functional diversity of lipid species and on the mechanisms that cells employ to sense and adjust their...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2012.07.033
更新日期:2012-08-31 00:00:00
abstract::A monoclonal antibody (mAb) has been produced which reacts with human mitofilin, a mitochondrial inner membrane protein. This mAb immunocaptures its target protein in association with six other proteins, metaxins 1 and 2, SAM50, CHCHD3, CHCHD6 and DnaJC11, respectively. The first three are outer membrane proteins, CHC...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2007.06.052
更新日期:2007-07-24 00:00:00
abstract::Tobacco SABP2, a 29kDa protein catalyzes the conversion of methyl salicylic acid (MeSA) into salicylic acid (SA) to induce SAR. Pretreatment of plants with acibenzolar-S-methyl (ASM), a functional analog of salicylic acid induces systemic acquired resistance (SAR). Data presented in this paper suggest that SABP2 catal...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.06.046
更新日期:2010-08-04 00:00:00
abstract::The effect of all possible codons corresponding to the second and third amino acid (isoleucine and threonine) on the expression level of hirudin in E. coli has been analysed. These levels could not be correlated with changes in primary and secondary mRNA structure. A decrease in the rate of synthesis and of product ac...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81164-j
更新日期:1990-08-20 00:00:00
abstract::Oligonucleotide-peptide conjugates have several applications, including their potential use as improved antisense agents for interfering with the RNA function within cells. In order to provide robust and generally applicable conjugation chemistry, we developed a novel approach of fragment coupling of pre-synthesized p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00921-7
更新日期:1999-07-30 00:00:00
abstract::After initial purification of ovine placental lactogen (oPL) using the procedures described previously [(1976) Endocrinology 98, 65-75], the oPL preparation was further purified by high-performance liquid chromatography (HPLC) using an anionic exchange column (Bio-Sil TSK DEAE-2-SW). Two forms of oPL with different re...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80491-4
更新日期:1986-04-21 00:00:00
abstract::We have identified and characterized a genomic DNA fragment containing the coding sequences corresponding to the human gamma-glutamyltransferase type 1 mRNA. The coding part of the gene spans over 16 kb and comprises 12 exons and 11 introns exhibiting a similar organization as for the mouse and rat GGT genes. The exon...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00950-8
更新日期:1998-08-28 00:00:00
abstract::A gene encoding an antifreeze protein (AFP) was isolated from carrot (Daucus carota) using sequence information derived from the purified protein. The carrot AFP is highly similar to the polygalacturonase inhibitor protein (PGIP) family of apoplastic plant leucine-rich repeat (LRR) proteins. Expression of the AFP gene...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00280-x
更新日期:1999-03-26 00:00:00
abstract::The effect of angiotensin II (Ang II) on the early growth response gene-1 (Egr-1) mRNA, on the Egr-1 protein and on the phosphoinositide PI turnover signalling system was investigated in the presence and absence of EXP3174, a potent non-peptide Ang II receptor antagonist. Ang II induced an accumulation of 3.4 kb Egr-1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)81423-j
更新日期:1992-11-23 00:00:00
abstract::The UDP-GlcNAc:lysosomal enzyme, N-acetylglucosamine-1-phosphotransferase (GlcNAc-1-PT), is an α2 β2 γ2 hexamer that mediates the initial step in the formation of the mannose 6-phosphate targeting signal on newly synthesized lysosomal acid hydrolases. The GNPTAB gene encodes the 1256 amino acid long α/β precursor whic...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.12525
更新日期:2017-01-01 00:00:00
abstract::The rate of whole cell H2O2 metabolism in several salivarian and stercorarian trypanosomes and Leishmania species was measured. These cells metabolized H2O2 at rates between 2.3 and 48.2 nmol/10(8) cells per min depending upon the species employed. H2O2 metabolism was largely insensitive to NaN3, implying that typical...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(87)80968-7
更新日期:1987-09-14 00:00:00
abstract::An efficient sensing of danger and a rapid activation of the immune system are crucial for the survival of plants. Conserved pathogen/microbe-associated molecular patterns (PAMPs/MAMPs) and endogenous molecular patterns, which are present only when the tissue is infected or damaged (damage-associated molecular pattern...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2011.04.043
更新日期:2011-06-06 00:00:00
abstract::The PDE4 catalytic machinery comprises, in part, two divalent cations in a binuclear motif. Here we report that PDE4A4 expressed in Sf9 cells exhibits a biphasic Mg(2+) dose-response (EC(50) of 0.15 and >10 mM) in catalyzing cAMP hydrolysis. In vitro phosphorylation of PDE4A4 by the PKA-catalytic subunit increases the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02259-7
更新日期:2002-02-13 00:00:00
abstract::We identified a gene encoding a novel secreted protein in mice, humans, and zebrafish. As the protein of 222 amino acids is similar to Brorin, a secreted BMP antagonist, which is a member of the Chordin family, we named it Brorin-like. Recombinant Brorin-like protein weakly but significantly inhibited the activity of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2009.10.044
更新日期:2009-11-19 00:00:00
abstract::Posttranslational modifications of circadian oscillator components are crucial for the generation of circadian rhythms. Among those phosphorylation plays key roles ranging from regulating degradation, complex formation, subcellular localization and activity. Although most of the known clock proteins are phosphoprotein...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2011.02.038
更新日期:2011-05-20 00:00:00
abstract::Micromolar concentrations of arachidonic acid cause in Ca2+ loaded heart mitochondria matrix swelling and Ca2+ release. These effects appear to be unrelated to the classical membrane permeability transition (MPT), as they are CsA insensitive, membrane potential independent and can also be activated by Sr2+. Atractylos...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.12.090
更新日期:2006-02-06 00:00:00
abstract::In this study, we demonstrated that Ent-11alpha-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F) had stronger cytotoxicity against MKN-45, a gastric cancer cell line bearing wild-type p53 than MKN-28, another gastric cancer cell line containing missense mutation in p53. The rapid increase of ROS level was involved in the me...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2005.01.050
更新日期:2005-02-28 00:00:00
abstract::Seven peptides from subunit 9 (S9) of the human 26S proteasome were sequenced and this information was used to clone a HeLa cDNA that encodes the 46 kDa subunit. Rabbit polyclonal antisera were made against a ubiquitin fusion protein containing 12 amino acids from S9 and against a full-length S9 expressed in E. coli. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00126-9
更新日期:1997-03-10 00:00:00
abstract::A cDNA coding for a new subunit of soluble guanylyl cyclase with a calculated molecular mass of 81.7 kDa was cloned and sequenced. On the basis of sequence homology, the new subunit appears to be an isoform of the alpha 1-subunit and was designated alpha 2 as the new subunit is very similar to the alpha 1-subunit in t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80871-y
更新日期:1991-11-04 00:00:00
abstract::A functional NAD molecule was immobilized at the active site of Alcohol dehydrogenase within a proteic membrane. The presence and the functionality of the cofactor was checked by fluorescence analysis. The dehydrogenase NAD membrane does not require addition of soluble cofactor for its activity. The system represents ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(78)80970-3
更新日期:1978-10-15 00:00:00
abstract::CXCR2 plays a key role in protecting the integrity of the endothelium. Emerging evidence has demonstrated that the long ncRNAs (lncRNA) Human metastasis associated lung adenocarcinoma transcript 1 (MALAT1) participates in the regulation of the pathophysiological processes. However, whether there is crosstalk between C...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.08.046
更新日期:2015-10-07 00:00:00
abstract::Several analogues of heat-stable enterotoxins (STh and STp) produced by enterotoxigenic Escherichia coli were synthesized. Peptides (STh[6-18] and STp[5-17]) consisting of 13 amino acid residues from the Cys residue near the N-terminus to the Cys residue near the C-terminus and linked by three disulfide bonds had the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)81129-7
更新日期:1985-02-11 00:00:00