Abstract:
:In this study, we demonstrated that Ent-11alpha-hydroxy-15-oxo-kaur-16-en-19-oic-acid (5F) had stronger cytotoxicity against MKN-45, a gastric cancer cell line bearing wild-type p53 than MKN-28, another gastric cancer cell line containing missense mutation in p53. The rapid increase of ROS level was involved in the mechanism of cytotoxicity. Classical features of apoptosis induced by 5F were observed in MKN-45 cells only or more significant in MKN-45 cells than MKN-28 cells. Translocation of Bax from cytosol to mitochondria, reduction of delta psi m and DNA fragmentation were induced by 5F in the p53-dependent manner. We conclude that the expression of Bax and its downstream molecules requires the presentation of a wild-type p53 in the cells treated by 5F.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Liu Z,Ng EK,Liang NC,Deng YF,Leung BC,Chen GGdoi
10.1016/j.febslet.2005.01.050keywords:
subject
Has Abstractpub_date
2005-02-28 00:00:00pages
1477-87issue
6eissn
0014-5793issn
1873-3468pii
S0014-5793(05)00151-1journal_volume
579pub_type
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