Abstract:
:The conformation change of Thermus thermophilus tRNA(1Ile) upon complex formation with T. thermophilus elongation factor Tu (EF-Tu) was studied by analysis of the circular dichroism (CD) bands at 315 nm (due to the 2-thioribothymidine residue in the T-loop) and at 295 nm (due to the core structure of tRNA). Formation of the ternary complex of isoleucyl-tRNA(1Ile) and EF-Tu.GTP increased the intensities of these CD bands, indicating stabilization of the association between the T-loop and the D-loop and also a significant conformation change of the core region. Upon complex formation of EF-Tu.GTP and uncharged tRNA, however, the conformation of the core region is not changed, while the association of the two loops is still stabilized. On the other hand, the binding with EF-Tu.GDP does not appreciably affect the conformation of isoleucyl-tRNA or uncharged tRNA. These indicate the importance of the gamma-phosphate group of GTP and the aminoacyl group in the formation of the active complex of aminoacyl-tRNA and EF-Tu.GTP.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Haruki M,Matsumoto R,Hara-Yokoyama M,Miyazawa T,Yokoyama Sdoi
10.1016/0014-5793(90)81414-jsubject
Has Abstractpub_date
1990-04-24 00:00:00pages
361-4issue
2eissn
0014-5793issn
1873-3468journal_volume
263pub_type
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