Abstract:
:We investigated insulin's effect on intestinal lipid, transport and, particularly, the biogenesis of apolipoproteins crucial to lipoprotein secretion. Adding insulin (3 mU) to the serum-free medium of cultured jejunal explants from human fetuses (17-20 weeks) reduced triglyceride and chylomicron production and inhibited apo B-48 and apo B-100 secretion. When apo B mRNA was assayed by RT-PCR and its editing by primer extension, no change was detectable following the addition of insulin. HDL lipid content, apo A-1 synthesis and RNA level were unaffected by insulin. Collectively, these results suggest that the insulin-stimulated decline in intestinal chylomicron output may involve apo B co- or post-translational modifications.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Levy E,Sinnett D,Thibault L,Nguyen TD,Delvin E,Ménard Ddoi
10.1016/0014-5793(96)00896-4subject
Has Abstractpub_date
1996-09-16 00:00:00pages
253-8issue
2-3eissn
0014-5793issn
1873-3468pii
0014-5793(96)00896-4journal_volume
393pub_type
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