Unusual enzyme characteristics of aspartyl-tRNA synthetase from hyperthermophilic archaeon Pyrococcus sp. KOD1.

abstract：:We quantitatively measured protective antigen (PA) binding to human cells targeted by anthrax lethal toxin (LT). Affinities were less than 50 nM for all cells, but differentiated cells (macrophages and neutrophils) had significantly increased PA binding and endothelial cells demonstrated the most binding. Combined wit...

journal_title：FEBS letters

authors： Deshpande A,Hammon RJ,Sanders CK,Graves SW

更新日期：2006-07-24 00:00:00

abstract：:The class A Ca2+ channel alpha 1 subunit (alpha 1A) was expressed in Xenopus oocytes alone or in combination with the beta 1b, beta 2a, beta 3, or beta 4 subunit. Analysis of voltage-dependent activation and inactivation in the presence of 1.8 mM external Ca2+ showed an hyperpolarising shift of both relations when com...

journal_title：FEBS letters

authors： Cens T,Mangoni ME,Nargeot J,Charnet P

更新日期：1996-08-12 00:00:00

abstract：:We have investigated the importance of the degree of sialylation when an acute phase glycoprotein, alpha 1-proteinase inhibitor (alpha 1-Pi), was analysed both by affinity chromatography on concanavalin A (Con A)-Sepharose and by crossed immuno-affinoelectrophoresis (CIAE) using Con A in the first dimension. Human alp...

journal_title：FEBS letters

authors： Mallet B,Franc JL,Zattara MC

更新日期：1990-03-12 00:00:00

abstract：:The sequence of the HIV Nef protein has no significant homology to other proteins in the SwissProt database, and experimental data concerning its function are sparse and contradictory. Using a novel protein sequence comparison method, we find similarities between different Nef sequences and the alpha chain of human MH...

journal_title：FEBS letters

authors： Hobohm U,Sander C

更新日期：1993-11-01 00:00:00

abstract：:Free and membrane-bound polyribosomes were isolated from the livers of untreated and clofibrate-treated male C57B1/6 mice. The in vitro translation products were investigated in a rabbit reticulocyte cell-free system by immunoprecipitation of cytosolic epoxide hydrolase, catalase and albumin. The soluble forms of epox...

journal_title：FEBS letters

authors： Joste V,Meijer J

更新日期：1989-05-22 00:00:00

abstract：:Some pathogenic mutations associated with Alzheimer's disease are thought to affect structural-dynamic properties and the lateral dimerization of amyloid precursor protein (APP) in neuron membrane. Dimeric structure of APP transmembrane fragment Gln(686)-Lys(726) was determined in membrane-mimicking dodecylphosphochol...

journal_title：FEBS letters

authors： Nadezhdin KD,Bocharova OV,Bocharov EV,Arseniev AS

更新日期：2012-06-12 00:00:00

abstract：:The role of Shine-Dalgarno-like sequences in mRNAs from higher plant chloroplasts has not been analyzed experimentally so far. In vitro translation analysis has revealed that the Shine-Dalgarno-like sequence is essential for translation of tobacco chloroplast rps14 mRNA. Two RNA editing sites have been identified in t...

journal_title：FEBS letters

authors： Hirose T,Kusumegi T,Sugiura M

更新日期：1998-07-03 00:00:00

abstract：:Glycinebetaine (betaine), an osmoregulant in halophilic plants, stabilized the evolution of oxygen and the synthesis of ATP by thylakoid membranes from the cyanobacterium Synechocystis PCC6803 when it was present during the preparation and incubation of the thylakoid membranes. Moreover, betaine enhanced the evolution...

journal_title：FEBS letters

authors： Mamedov MD,Hayashi H,Wada H,Mohanty PS,Papageorgiou GC,Murata N

更新日期：1991-12-09 00:00:00

abstract：:The dependence of protein splicing on conserved residues of the Cne PRP8 intein was assessed by alanine scanning mutagenesis in a foreign protein context. Corroboration was obtained for the involvement of residues at the splice junctions and of the conserved threonine and histidine of motif B. Five additional residues...

journal_title：FEBS letters

authors： Pearl EJ,Tyndall JD,Poulter RT,Wilbanks SM

更新日期：2007-06-26 00:00:00

abstract：:We show here that phosphorylation of the insulin receptor and insulin receptor substrate-1 is increased when suspended cells are replated on fibronectin. This is not due to decreased numbers of cell surface receptors, alteration of insulin binding, or stimulation of a phosphatase activity in non-adherent cells. Expres...

journal_title：FEBS letters

authors： El Annabi S,Gautier N,Baron V

更新日期：2001-11-02 00:00:00

abstract：:Brain-type creatine kinase B-CK (EC 2.7.3.2) was purified from several chicken tissues, e.g. cardiac muscle, brain, gizzard and retina. Two major monomeric chicken B-CK subunits, designated Bb (basic) and Ba (acidic), which differ in isoelectric point, were separated by chromatofocusing in the presence of 8 M urea on ...

journal_title：FEBS letters

authors： Quest AF,Eppenberger HM,Wallimann T

更新日期：1990-03-26 00:00:00

abstract：:Physiological and pathological states that are associated with elevated plasma fatty acids (FAs) increase uncoupling protein 2 (UCP2) mRNA in white adipose tissue and UCP3 mRNA in skeletal muscle and heart. A direct effect of unsaturated fatty acids from all classes has been shown in various cultured cells. There is e...

journal_title：FEBS letters

authors： Thompson MP,Kim D

更新日期：2004-06-18 00:00:00

abstract：:In this study we identify the molecules involved in the MAPK signal transduction pathway (Ras, Raf-1, Mek, Mek-P and MAPK) in highly purified endosomal fractions isolated from rat liver. Biochemical analysis shows that only the early-sorting endocytic compartment contains activated Raf-1 and Mek. Finally, the exogenou...

journal_title：FEBS letters

authors： Pol A,Calvo M,Enrich C

更新日期：1998-12-11 00:00:00

abstract：:The nuclear gene OXA1 was first isolated in Saccharomyces cerevisiae and found to be required at a post-translational step in cytochrome c oxidase biogenesis, probably at the level of assembly. Mutations in OXA1 lead to a complete respiratory deficiency. The protein Oxa1p is conserved through evolution and a human hom...

journal_title：FEBS letters

authors： Altamura N,Capitanio N,Bonnefoy N,Papa S,Dujardin G

更新日期：1996-03-11 00:00:00

abstract：:The kinetic properties of dUTPase from equine infectious anemia virus (EIAV) were investigated. K(M) (1.1 +/- 0.1 microM) and k(cat) (25 s(-1)) were found to be independent of pH in the neutral pH range. Above pH 8.0, K(M) increases slightly. Below pH 6.0, the enzyme is rapidly deactivated. Detergent was found to enha...

journal_title：FEBS letters

authors： Nord J,Larsson G,Kvassman JO,Rosengren AM,Nyman PO

更新日期：1997-09-08 00:00:00

abstract：:It is known that the DNA binding Runt domain of the AML1/Runx1 transcription factor coordinates Cl(-) ions. In this paper we have determined Cl(-) binding affinities of AML1 by (35)Cl nuclear magnetic resonance (NMR) linewidth analysis. The Runt domain binds Cl(-) with a dissociation constant (K(d,Cl)) of 34 mM. If CB...

journal_title：FEBS letters

authors： Wolf-Watz M,Bäckström S,Grundström T,Sauer U,Härd T

更新日期：2001-01-12 00:00:00

abstract：:Preformed phosphatidylethanolamine-containing liposomes were thiolated with 2-iminothiolane (Traut's reagent) and subsequently activated by mixed disulfide formation with 5,5'-dithiobis(2-nitrobenzoic acid). Up to 65% of amino groups of the outer liposomal lamella, corresponding to 230 SH-groups per vesicle, were modi...

journal_title：FEBS letters

authors： Lasch J,Niedermann G,Bogdanov AA,Torchilin VP

更新日期：1987-04-06 00:00:00

abstract：:To study the fluctuations of cGMP in living cells through changes of energy transfer of dissociable fluorescence labeled subunits, we constructed a cGMP-sensitive probe by combining the N-terminus of the type I regulatory subunit of cAMP-dependent protein kinase (PKA) with the cGMP binding sites of cGMP-dependent prot...

journal_title：FEBS letters

authors： Wild N,Herberg FW,Hofmann F,Dostmann WR

更新日期：1995-11-06 00:00:00

abstract：:Leaves and chloroplasts from Arabidopsis plants with increased amounts of PsbS protein showed the same percentage increase in nonphotochemical quenching in comparison to the wild type both in the presence and absence of zeaxanthin. The absorption change at 525-535 nm was also more pronounced in both cases. It is sugge...

journal_title：FEBS letters

authors： Crouchman S,Ruban A,Horton P

更新日期：2006-04-03 00:00:00

abstract：:Arabidopsis thaliana L. produces flavonoid pigments, i.e. flavonols, anthocyanidins and proanthocyanidins, from dihydroflavonol substrates. A small family of putative flavonol synthase (FLS) genes had been recognized in Arabidopsis, and functional activity was attributed only to FLS1. Nevertheless, other FLS activitie...

journal_title：FEBS letters

authors： Preuss A,Stracke R,Weisshaar B,Hillebrecht A,Matern U,Martens S

更新日期：2009-06-18 00:00:00

abstract：:The von Hippel-Lindau (VHL) tumor suppressor gene regulates the extracellular matrix by controlling fibronectin deposition. To identify novel VHL target genes, we subjected mRNA from VHL-deficient RCC cells (786-0-pRC) and a transfectant re-expressing wildtype VHL (786-0-VHL) to differential expression profiling. Amon...

journal_title：FEBS letters

authors： Bluyssen HA,Lolkema MP,van Beest M,Boone M,Snijckers CM,Los M,Gebbink MF,Braam B,Holstege FC,Giles RH,Voest EE

更新日期：2004-01-02 00:00:00

abstract：:The contribution of hepatic glycogen to lipogenesis was studied in isolated, intact rat hepatocytes. To establish its importance as a substrate for lipogenesis, the glycogen of isolated hepatocytes was prelabelled with 14C from glucose. Evidence is presented that neither glucose nor glycogen constitute major sources o...

journal_title：FEBS letters

authors： Geelen MJ,Hindriks GA

更新日期：1984-09-17 00:00:00

abstract：:Lysophosphatidic acid (LPA) exerts multiple biological functions through G protein-coupled receptors (EDG2/LPA(1), EDG4/LPA(2), and EDG7/LPA(3)) and is present in serum where it is associated with albumin. In this study we examined LPA activity in various biological fluids by measuring the LPA-induced increase in the ...

journal_title：FEBS letters

authors： Hama K,Bandoh K,Kakehi Y,Aoki J,Arai H

更新日期：2002-07-17 00:00:00

abstract：:The interferon inducible transmembrane (IFITM) proteins mediate several cellular processes such as homotypic cell adhesion functions of interferons (IFNs) and cellular anti-proliferative activities. We show that the BAF complex-mediated induction of IFITM3 is dependent on binding of the transcriptional enhancer factor...

journal_title：FEBS letters

authors： Cuddapah S,Cui K,Zhao K

更新日期：2008-01-23 00:00:00

abstract：:The proteolysis of beta-amyloid (Abeta) requires neprylisin, an enzyme that has been shown as reduced in Alzheimer's disease (AD). We investigated whether a decrease in neprilysin levels contributes to the accumulation of amyloid deposits not only in AD but also in the normal aging. We analyzed neprilysin mRNA and pro...

journal_title：FEBS letters

authors： Russo R,Borghi R,Markesbery W,Tabaton M,Piccini A

更新日期：2005-11-07 00:00:00

abstract：:A Synechococcus PCC7942 mutant in which the psbO gene was inactivated by insertion of a chloramphenicol interposon and which did not contain any detectable manganese stabilizing protein in immunoblot experiments, was constructed. Such a Synechococcus mutant was able to grow under photoautotrophic conditions. Isolated ...

journal_title：FEBS letters

authors： Bockholt R,Masepohl B,Pistorius EK

更新日期：1991-12-02 00:00:00

abstract：:The higher-order structure of G protein-coupled receptors (GPCRs) in membranes may involve dimerization and formation of even larger oligomeric complexes. Here, we have investigated the organization of the prototypical GPCR rhodopsin in its native membrane by electron and atomic force microscopy (AFM). Disc membranes ...

journal_title：FEBS letters

authors： Fotiadis D,Liang Y,Filipek S,Saperstein DA,Engel A,Palczewski K

更新日期：2004-04-30 00:00:00

abstract：:Serotonin, a known neurotransmitter, also functions as an angiokine to promote angiogenesis. The majority of serotonin in the human body is stored in platelets, and platelet aggregation leads to significant release of serotonin in thrombotic tumor environment. We have investigated serotonin signaling in human endothel...

journal_title：FEBS letters

authors： Zamani A,Qu Z

更新日期：2012-07-30 00:00:00

abstract：:The omega3 fatty-acid desaturases: FAD7 and FAD8 (plastid) and FAD3 (reticular) are responsible for trienoic fatty-acid (TA) production in plants. The expression of these enzymes seemed to be regulated differently in response to light. Darkness leads to a decrease in total TA level. Under such conditions, FAD3 and FAD...

journal_title：FEBS letters

authors： Collados R,Andreu V,Picorel R,Alfonso M

更新日期：2006-09-04 00:00:00

abstract：:Fourier transform infrared spectroscopy is used to estimate the secondary structure of bacteriorhodopsin dissolved in chloroform-methanol (1:1 v/v), 0.1 M LiClO4. Curve-fitting of the deconvolved spectra in the amide I region shows that the total content of alpha-helices, reverse turns and beta-sheets are similar to t...

journal_title：FEBS letters

authors： Torres J,Padrós E

更新日期：1993-02-22 00:00:00