Abstract:
:Two-dimensional hydrophobic clusters analysis (HCA) was used to compare the distribution of hydrophobic clusters along various actin sequence. HCA-deduced patterns were not altered by amino-acid variations throughout the evolution of actin and we observed similar hydrophobic motifs comprising myosin subfragment-1 ATP-independent binding sites. HCA suggested the presence of two groups of identical hydrophobic motifs (A1 and A2) which bound on each side of the S1 (63 kDa-31 kDa) connecting segment in relation with two actin monomers. This connection is important in communications between actin- and nucleotide-binding sites. We postulate that some relation and message between the two motifs A1 and A2 take place through myosin subfragment-1 (63 kDa-31 kDa) connecting segment.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Labbé JP,Boyer M,Benyamin Ydoi
10.1016/0014-5793(95)01044-fsubject
Has Abstractpub_date
1995-10-16 00:00:00pages
221-4issue
3eissn
0014-5793issn
1873-3468pii
0014-5793(95)01044-Fjournal_volume
373pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::We show that inverted membrane vesicles from Corynebacterium glutamicum, a Gram-positive bacterium, are able to generate and maintain an electrochemical gradient of protons in response to the addition of NADH. This result indicates that the respiratory chain is intact and that the vesicles are reasonably impermeable t...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)01245-8
更新日期:1994-12-12 00:00:00
abstract::α-Glucosidase hydrolyzes α-glucosides and transfers α-glucosyl residues to an acceptor through transglucosylation. In this study, GH13_31 α-glucosidase BspAG13_31A with high transglucosylation activity is reported in Bacillus sp. AHU2216 and biochemically and structurally characterized. This enzyme is specific to α-(1...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1002/1873-3468.13126
更新日期:2018-07-01 00:00:00
abstract::The -proteobacterium Wolinella succinogenes grows anaerobically by respiratory nitrite ammonification but not by denitrification. Nevertheless, it is capable of N(2)O reduction to N(2). Recently, the genome sequence of W. succinogenes revealed a nos gene cluster with intriguing features encoding a new type of N(2)O re...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2004.05.060
更新日期:2004-07-02 00:00:00
abstract::To determine the amino acid residues required for the signal-transducing activity of the human c-Ha-Ras protein, we introduced point mutations at residues 45-54 near the 'effector region' (residues 32-40). We transfected PC12 cells with these mutant genes and also micro-injected the mutant proteins, bound with an unhy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80665-p
更新日期:1991-12-09 00:00:00
abstract::The two tryptophan residues, Trp-248 and Trp-330, in tryptophan indole-lyase (tryptophanase) from E. coli have been separately mutated to phenylalanine using site-directed mutagenesis. Both single tryptophan mutant enzymes have full catalytic activity, but exhibit different fluorescence and near-UV circular dichroism ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)81011-c
更新日期:1990-07-30 00:00:00
abstract::Tumour necrosis factor-alpha (TNFalpha) is a multifunctional cytokine that exerts a myriad of biological actions in numerous different tissues including adipocytes through its two distinct cell surface receptors. To address the role of each TNF receptor in the biological actions of TNFalpha in adipocytes, we have deve...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)01250-3
更新日期:2000-03-03 00:00:00
abstract::We describe the use of a phage-displayed random pentadecamer peptide library for searching glycosphingolipid mimicking peptides. Two phage clones (AD-1 and AD-2) were selected by biopanning using monoclonal antibody AD117m, directed to lactotetraosylceramide (Lc4Cer). The amino acid sequences of the selected clones sh...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01386-0
更新日期:1997-11-24 00:00:00
abstract::Cytochrome P-450scc (P-450scc), a cholesterol side-chain cleavage enzyme from bovine adrenocortical mitochondria, has been crystallized for the first time. Upon removal of glycerol from the solution of the native enzyme complexed with pyridoxal 5'-phosphate (PLP) by microdialysis against distilled water, reddish and p...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81350-4
更新日期:1988-06-06 00:00:00
abstract::IscA homologs are known to be involved in iron-sulfur cluster formation in various organisms. Recombinant proteins of two IscA homologs from the cyanobacterium Synechocystis PCC 6803, designated SLR1417 and SLR1565, were purified. The absorption spectrum of purified SLR1565 was typical for [2Fe-2S] cluster-containing ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02736-9
更新日期:2002-05-22 00:00:00
abstract::Turnip yellow mosaic virus (TYMV) is a positive-strand RNA virus able to infect Arabidopsis thaliana. To establish a TYMV infection system in Arabidopsis cell culture, TYMV replicons with the capsid protein gene replaced by a reporter gene expressing the Sh ble protein conferring zeocin resistance were used to transfe...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.12.045
更新日期:2007-01-23 00:00:00
abstract::Mammalian pancreatic phospholipase A2 (PLA2-I) has its specific receptor through which PLA2-I induces a variety of biological responses. In this study, a fundamental relationship between the enzymatic and the receptor-binding activities of PLA2-I was investigated. The specific binding of PLA2-I to the receptor was fou...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)80149-o
更新日期:1993-06-21 00:00:00
abstract::The aim of this study was to evaluate, using a rat model of balloon angioplasty, whether prostaglandin (PG) J(2) and 2-cyclopenten-1-one are able to reduce restenosis. We found that both PGJ(2) and 2-cyclopenten-1-one, administered by local application on carotid arteries, caused a dose-dependent inhibition of neointi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00873-1
更新日期:2003-10-09 00:00:00
abstract::Contribution of sphingosine kinase (SPK)-catalyzed production of sphingosine-1-phosphate (SPP), in comparison to phospholipase C (PLC), to Ca(2+) signalling by epidermal growth factor (EGF) was studied in two HEK-293 cell clones (HEK2 and HEK3), expressing functional EGF receptors and exhibiting release of stored Ca(2...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01463-5
更新日期:1999-11-19 00:00:00
abstract::The octameric ring protein, Translin, demonstrates marked similarities to the family of helicase enzymes regarding its quaternary organization and dimerization of subunits. Here we show that the level of Translin closely parallels the proliferative state in various cell types. Expression is periodic during the cell cy...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)03095-8
更新日期:2002-08-14 00:00:00
abstract::The genes for the hypothalamic hormones vasopressin and oxytocin are located in close proximity to each other within the rat genome. They are separated by only approx. 11 kbp of DNA sequence and oriented in such a way that their transcription occurs on opposite DNA strands. Although the two genes are structurally very...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(88)81003-2
更新日期:1988-12-19 00:00:00
abstract::N-Oligosaccharyltransferase catalyzes the N-glycosylation of asparagine residues of nascent polypeptide chains in the endoplasmic reticulum, a pathway highly conserved in all eukaryotes. An enzymatically active complex was isolated from microsomal membranes from Saccharomyces cerevisiae, which is composed of four prot...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00356-4
更新日期:1994-05-09 00:00:00
abstract::Myometrial membranes, obtained from estrogen-dominated (day 0) rat uteri, were immunoblotted with antiserum (SG1), which recognizes the alpha subunits of both Gi1 and Gi2, with antiserum (LE2) specific for Gi2 alpha, and with I3B antiserum, specific for Gi3 alpha. The data revealed the absence of detectable levels of ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80070-j
更新日期:1991-01-14 00:00:00
abstract::The Protein Data Bank (PDB) was established in 1971 as a repository for the three dimensional structures of biological macromolecules. Since then, more than 85000 biological macromolecule structures have been determined and made available in the PDB archive. Through analysis of the corpus of data, it is possible to id...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2012.12.029
更新日期:2013-04-17 00:00:00
abstract::Long-chain fatty acid amides are cell-signaling lipids identified in mammals and, recently, in invertebrates, as well. Many details regarding fatty acid amide metabolism remain unclear. Herein, we demonstrate that Drosophila melanogaster is an excellent model system for the study long-chain fatty acid amide metabolism...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2014.02.051
更新日期:2014-05-02 00:00:00
abstract::FoxM1 (previously named WIN, HFH-11 or Trident) is a Forkhead box (Fox) transcription factor widely expressed in proliferating cells. Various findings, including a recent analysis of FoxM1 knockout mice, suggest that FoxM1 is required for normal S-M coupling during cell cycle progression. To study the regulatory role ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02915-5
更新日期:2001-10-19 00:00:00
abstract::Duchenne muscular dystrophy (DMD) is a fatal disorder caused by absence of functional dystrophin protein. Compensation in dystrophin-deficient (mdx) mice may be achieved by overexpression of its fetal paralogue, utrophin. Strategies to increase utrophin levels by stimulating promoter activity using small compounds are...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.13099
更新日期:2018-06-01 00:00:00
abstract::1-Farnesylpyridinium (FPy), an analog of isoprenoid farnesol, initially induced morphological changes similar to those of typical apoptosis in human leukemia HL-60 cells but FPy-treated cells were characterized by the absolute absence of final apoptotic events such as fragmentation into apoptotic bodies. FPy-induced c...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(02)02373-6
更新日期:2002-03-13 00:00:00
abstract::A central step in xanthone biosynthesis is the formation of the C13 skeleton, i.e. an intermediate benzophenone. Biosynthesis of 2,3',4,6-tetrahydroxybenzophenone from m-hydroxybenzoyl-CoA and malonyl-CoA was shown in cell-free extracts from cultured cells of Centaurium erythraea. The enzyme catalyzing this reaction w...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(96)00265-7
更新日期:1996-04-01 00:00:00
abstract::In addition to the classical functions of the Golgi in membrane transport and glycosylation, the Golgi apparatus of mammalian cells is now recognised to contribute to the regulation of a range of cellular processes, including mitosis, DNA repair, stress responses, autophagy, apoptosis and inflammation. These processes...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1002/1873-3468.13567
更新日期:2019-09-01 00:00:00
abstract::D-Galactopyranosyl residues were coupled to poly(L-lysine) and the antiviral agents arabinofuranosyladenine 5'-monophosphate (ara-AMP) and acyclovir were conjugated with this glycosylated polymer. In mice the ara-AMP conjugate accomplished a selective drug delivery to liver cells. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80742-6
更新日期:1986-07-28 00:00:00
abstract::Mitochondrial ATP synthase, a major ATP supplier in respiring cells, should be regulated in amount and in activity to respond to the varying demands of cells for ATP. We screened 80 protein kinase inhibitors and found that HeLa cells treated with four inhibitors exhibited reduced mitochondrial ATP synthesis activity. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2013.10.012
更新日期:2013-11-29 00:00:00
abstract::The glycogen branching enzyme from Vibrio vulnificus (VvGBE) transfers short side chains (DP 3-5) significantly greater than any other bacterial glycogen branching enzyme (GBE). To elucidate the role of the N-domain of VvGBE in the unique branching pattern, domain-truncated (N1 and N) and N1-domain-swapped (with VvGBE...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2015.03.011
更新日期:2015-04-28 00:00:00
abstract::The action of high-intensity ultraviolet pulse laser radiation on a 161 bp fragment of pBR 322 DNA (EcoRI-MspI fragment) was studied. At doses up to 5 X 10(18) photons/cm2 the N-glycosidic bond splitting is negligible. The action of hot piperidine on irradiated DNA leads to chain splitting at the residues, modified vi...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80894-2
更新日期:1985-08-19 00:00:00
abstract::A 1000-bp fragment of Enterococcus hirae genomic DNA was amplified by the polymerase chain reaction method, using the oligonucleotide primers designed from amino acid sequences of both amino-terminal and a tryptic fragment of the Na(+)-ATPase alpha-subunit in this organism. DNA sequencing of this product revealed that...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80835-q
更新日期:1991-11-04 00:00:00
abstract::DNA complementary to chicken ultimobranchial gland mRNA was cloned into the Pst I site of plasmid vector pBR322. A plasmid was selected by DNA-mRNA hybridization. We report here the partial nucleotide sequence of chicken calcitonin mRNA and the deduced complete amino acid sequence of chicken calcitonin. ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80242-8
更新日期:1985-01-21 00:00:00