Abstract:
:Contribution of sphingosine kinase (SPK)-catalyzed production of sphingosine-1-phosphate (SPP), in comparison to phospholipase C (PLC), to Ca(2+) signalling by epidermal growth factor (EGF) was studied in two HEK-293 cell clones (HEK2 and HEK3), expressing functional EGF receptors and exhibiting release of stored Ca(2+) by intracellular SPP. In HEK3 cells, EGF increased [Ca(2+)](i) and stimulated both, SPK and PLC. [Ca(2+)](i) increase, but not PLC stimulation, was strongly reduced by SPK inhibition. In HEK2 cells, EGF similarly stimulated PLC, but did not increase [Ca(2+)](i) or stimulate SPK, suggesting that intracellular SPP production plays a major role for Ca(2+) signalling by EGF in HEK-293 cells.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Meyer zu Heringdorf D,Lass H,Kuchar I,Alemany R,Guo Y,Schmidt M,Jakobs KHdoi
10.1016/s0014-5793(99)01463-5keywords:
subject
Has Abstractpub_date
1999-11-19 00:00:00pages
217-22issue
3eissn
0014-5793issn
1873-3468pii
S0014-5793(99)01463-5journal_volume
461pub_type
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