Abstract:
:Incubation of HER14 cells with phorbol myristate acetate (PMA) decreases epidermal growth factor (EGF)-dependent protein tyrosine phosphorylation, except for a 40-kDa MAP kinase II-like protein, whose tyrosine phosphorylation is further enhanced. The inhibitory effect of PMA on EGF-dependent protein tyrosine phosphorylation is reversed if cell are pre-incubated with a combination of Na3VO4 and NaF, two known inhibitors of protein tyrosine phosphatase activity. Protein tyrosine phosphatase activity of cell homogenate was measured on immunopurified EGF receptor, and was found to be enhanced in PMA-treated cells. These data suggest that the inhibitory effect of PMA on EGF-dependent protein tyrosine phosphorylation in HER14 cells may be mediated by protein tyrosine phosphatase activity.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Errasfa M,Stern Adoi
10.1016/0014-5793(94)80374-9subject
Has Abstractpub_date
1994-02-14 00:00:00pages
7-10issue
1-2eissn
0014-5793issn
1873-3468pii
0014-5793(94)80374-9journal_volume
339pub_type
杂志文章相关文献
FEBS LETTERS文献大全abstract::Analysis of the amino acid sequence of bovine brain myelin proteolipid reveals not only extensive internal homology, but also homology with portions of the myelin basic protein, the peripheral nerve myelin protein, Po, and with the small proteolipid subunit of mitochondrial ATP synthase. These results suggest that the...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(83)80732-7
更新日期:1983-09-05 00:00:00
abstract::Poly(A)+ RNA isolated from post-mortem human hypothalami has been used to characterize the poly-protein precursors to vasopressin and oxytocin. Translation in a cell-free system and subsequent immuno-precipitation with antibodies raised against either vasopressin or neurophysin identified a product of Mr 19000 (prepro...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(85)80069-7
更新日期:1985-11-25 00:00:00
abstract::Carboxylesterase (EII') from Arthrobacter sp. KI72 has 88% homology to 6-aminohexanoate-dimer hydrolase (EII) and possesses ca. 0.5% of the level of 6-aminohexanoate-linear dimer (Ald)-hydrolytic activity of EII. To study relationship between Ald-hydrolytic and esterolytic activities, random mutations were introduced ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.08.031
更新日期:2006-09-18 00:00:00
abstract::Heparin Binding Hemagglutinin A (HBHA) is hitherto the sole virulence factor associated with tuberculosis dissemination from the lungs, the site of primary infection, to epithelial cells. We have previously reported the solution structure of HBHA, a dimeric and elongated molecule. Since oligomerisation of HBHA is asso...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2010.02.044
更新日期:2010-03-19 00:00:00
abstract::Heparin was found to stimulate the phosphorylation of histone H1 but not protamine sulfate catalyzed by Ca2+/phospholipid-dependent protein kinase (protein kinase C or PKC). The effect of heparin on histone H1 phosphorylation appeared to be due to an increase in phosphatidylserine affinity for PKC activation in the pr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)80533-9
更新日期:1991-05-06 00:00:00
abstract::CXCR4 (fusin) is a chemokine receptor which is involved as a coreceptor in gp120 binding to the cell surface. In this study we provide evidence that binding of gp120 triggers CXCR4 recruitment to glycosphingolipid-enriched microdomains. Scanning confocal microscopy showed a nearly complete localization of CXCR4 within...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(01)02830-7
更新日期:2001-09-28 00:00:00
abstract::The nef gene of human and simian immunodeficiency virus is a key factor in acquired immunodeficiency syndrome pathogenesis and virus replication. Several Nef-induced phenomena, including the down-regulation of CD4 molecule, have been previously reported. In this study, we have identified and cloned a novel cellular pr...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)01631-7
更新日期:1999-01-08 00:00:00
abstract::Two genes code for calcitonin gene-related peptides (CGRPs). One expresses by tissue-specific alternate splicing calcitonin and CGRP I mRNAs, the other CGRP II mRNA. Calcitonin is the marker of sporadic or hereditary human medullary thyroid carcinoma (MTC). CGRP II expression is not well established in normal or tumor...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80856-e
更新日期:1990-12-17 00:00:00
abstract::The mechanism of store-operated Ca2+ inflow in hepatocytes was investigated using fluo-3 and fura-2 to monitor changes in the concentration of intracellular free Ca2+ in single cells, and 1-(alpha-glycerophosphoryl)-myo-inositol 4,5-diphosphate, P4(5)-1-(2-nitrophenyl)ethyl ester ('caged' GPIP2) and 'caged' guanosine ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00481-1
更新日期:1994-06-13 00:00:00
abstract::The class III phosphatidylinositol 3-kinase (PI3K-III) complex and its phosphorylated lipid product phosphatidylinositol 3-phosphate (PtdIns3P) control the three topologically related membrane-involution processes autophagy, endocytosis, and cytokinesis. The activity of the catalytic unit of PI3K-III complex, the Vacu...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/j.febslet.2012.04.046
更新日期:2012-06-04 00:00:00
abstract::An antibody recognising acetylated core histones was used to immunoprecipitate chromatin fragments from proliferating human K562 cells and from cells induced to differentiate with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). The DNA of the acetylated chromatin was probed with sequences of platelet der...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(93)81601-u
更新日期:1993-12-20 00:00:00
abstract::Xenopus oocytes expressing neuronal alpha 1C, alpha 2 and beta 1b calcium channel subunit cDNAs were used in this study. During two-electric voltage clamp recording the oocyte was injected with 10-20 nl of a 100 mM BAPTA solution. Under these conditions, the endogenous Ca-activated Cl current was completely suppressed...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(94)00357-2
更新日期:1994-05-09 00:00:00
abstract::Ghrelin is a novel gastrointestinal peptide that stimulates growth hormone secretion, food intake, and body weight gain. Increased ghrelin secretion has been reported in such negative energy states as starvation and low body weight. We investigated the dynamics of ghrelin in rats with streptozotocin-induced diabetes, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00306-5
更新日期:2003-04-24 00:00:00
abstract::Ceramide-1-phosphate (CER-1-P) is emerging as a novel bioactive sphingolipid. It is formed by phosphorylation of ceramide catalyzed by ceramide kinase, and has been implicated in different cellular processes. Cer-1-P is mitogenic for fibroblasts, blocks apoptosis in macrophages, controls phagocytosis in neutrophils, a...
journal_title:FEBS letters
pub_type: 杂志文章,评审
doi:10.1016/s0014-5793(04)00211-x
更新日期:2004-03-26 00:00:00
abstract::Piericidin is a potent inhibitor of the mitochondrial and bacterial type I NADH-ubiquinone oxidoreductases (Complex I) and is considered to bind at or close to the ubiquinone binding site(s) of the enzyme. Piericidin-resistant mutants of the bacterium Rhodobacter capsulatus have been isolated and the present work demo...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(98)00719-4
更新日期:1998-07-10 00:00:00
abstract::Nine analogs of fusion peptide of influenza virus hemagglutinin whose membrane perturbation activity has been thoroughly tested [Murata et al. (1992) Biochemistry 31, 1986-1992; Murata et al. (1993) Biophys. J. 64, 724-734] were characterized by molecular modeling techniques with the aim of delineating any specific st...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)01505-7
更新日期:1999-11-26 00:00:00
abstract::The urokinase receptor (uPAR) on the cell surface plays an important role in extracellular proteolysis, cell migration and adhesion. Soluble uPAR (suPAR) has been recently discovered in plasma, but its origin is unclear. Our results now demonstrate that both unstimulated blood mononuclear and endothelial cells can rel...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(00)02312-7
更新日期:2000-12-15 00:00:00
abstract::The CA microsatellite repeat marker, D7S522, is located at the center of a approximately 1000 kb smallest common deleted region that is lost in many forms of human cancer. It has been proposed that a putative tumor suppressor gene lies in close proximity to D7S522, within this smallest common deleted region. However, ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(99)00365-8
更新日期:1999-04-09 00:00:00
abstract::The initial processes of platelet activation by platelet-activating factor (PAF) were observed by stopped-flow light scattering and fluorometry. The binding of PAF to rabbit platelets did not change the membrane fluidity, though it caused the removal of calcium from internal stores and induced concomitantly platelet s...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(86)80034-5
更新日期:1986-10-20 00:00:00
abstract::A metallothionein cDNA was generated from pea (Pisum sativum L.) roots, amplified by PCR and inserted into a plasmid for expression in E. coli. Purification of the resultant product generated 3 pools of cadmium-containing material after DEAE-cellulose chromatography. The amino acid composition of each was in excellent...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(91)81411-z
更新日期:1991-12-16 00:00:00
abstract::In exocrine acinar cells a variety of neurotransmitters (e.g. acetylcholine) stimulate phosphatidylinositol 4,5-bisphosphate hydrolysis elevating intracellular calcium to activate calcium-dependent membrane currents (outward K+ and inward Cl-). This study shows that in lacrimal acinar cells extracellular application o...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(90)80782-e
更新日期:1990-05-07 00:00:00
abstract::To identify the linear epitope for Fc-binding on the bovine IgG2 Fc receptor (boFcgamma2R), peptides derived from the membrane-distal extracellular domain (EC1) of boFcgamma2R corresponding to the homologous region of human FcalphaRI were synthesized. Binding of bovine IgG2 to the different peptides was tested by Dot-...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/j.febslet.2006.01.060
更新日期:2006-02-20 00:00:00
abstract::cDNAs encoding the isoform I alpha of the cGMP-dependent protein kinase were isolated from a bovine trachea smooth muscle cDNA library constructed in lambda gt10. The deduced protein sequence is identical with the protein sequence obtained by Edman degradation of the bovine lung enzyme [(1984) Biochemistry 23, 4207-42...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(89)81453-x
更新日期:1989-07-17 00:00:00
abstract::We have purified a form of protein phosphatase 1 (PP1) from HeLa cell nuclei, in which the phosphatase is complexed to a regulatory subunit termed p99. We report here the cloning and characterisation of the p99 component. p99 mRNA is widely expressed in human tissues and immunofluorescence analysis with anti-p99 antib...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)01485-3
更新日期:1997-12-22 00:00:00
abstract::Human liver contains hydrolytic activity toward 3 beta-glucosido-chenodeoxycholic acid. This beta-glucosidase activity, localized predominantly in the microsomal fraction, was optimally active in the presence of divalent metal ions close to pH 5.0 and was inhibited by EDTA. Kinetic parameters and other catalytic prope...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(92)80970-r
更新日期:1992-12-14 00:00:00
abstract::Anaerobic biosynthesis of carotenoids in the purple facultative photosynthetic bacterium Rhodobacter sphaeroides was studied using mass spectrometry. We have demonstrated that (18)O from H2(18)O was incorporated into the 1-methoxy group of spheroidene and spheroidenone, the two major carotenoids produced by this bacte...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(97)00006-9
更新日期:1997-02-10 00:00:00
abstract::Duchenne muscular dystrophy (DMD) is a fatal disorder caused by absence of functional dystrophin protein. Compensation in dystrophin-deficient (mdx) mice may be achieved by overexpression of its fetal paralogue, utrophin. Strategies to increase utrophin levels by stimulating promoter activity using small compounds are...
journal_title:FEBS letters
pub_type: 信件
doi:10.1002/1873-3468.13099
更新日期:2018-06-01 00:00:00
abstract::p38, which has been suggested to be a scaffold protein for the assembly of a macromolecular tRNA synthetase complex, contains a leucine zipper-like motif. To understand the importance of the leucine zipper-like motif of p38 (p38LZ) in macromolecular assembly, the p38LZ solution structure was investigated by circular d...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/s0014-5793(03)00362-4
更新日期:2003-05-08 00:00:00
abstract::Lactate dehydrogenase (LDH) and yeast alcohol dehydrogenase ( YADH ) are inactivated when attacked by hydroxy free radicals (OH). Organic molecules with a high rate constant of reaction with OH such as ascorbate or urate can compete with the enzymes for these strongly oxidising radicals. However, although 10(-3)M asco...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(84)81391-5
更新日期:1984-05-07 00:00:00
abstract::A technique has been developed to probe directly RecA-DNA interactions by the use of the fluorescent chromophore, (+)anti-benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide (BPDE), covalently attached to DNA. The 24-mer oligonucleotide 5'-d(CTACTAAACATGTACAAATCATCC) was specifically modified on the exocyclic nitrogen of the ...
journal_title:FEBS letters
pub_type: 杂志文章
doi:10.1016/0014-5793(95)00600-e
更新日期:1995-07-10 00:00:00