Abstract:
:The photoreduction of the quinone (Q) pool in the photosynthetic membrane of the purple bacterium Rhodobacter sphaeroides was investigated by steady-state and time-resolved Fourier transform infrared difference spectroscopy. The results are consistent with the existence of a homogeneous Q pool inside the chromatophore membrane, with a size of around 20 Q molecules per reaction center. IR marker bands for the quinone/quinol (Q/QH(2)) redox couple were recognized. QH(2) bands are identified at 1491, 1470, 1433 and 1388-1375 cm(-1). The 1491 cm(-1) band, which is sensitive to (1)H/(2)H exchange, is assigned to a C-C ring mode coupled to a C-OH mode. A feature at approximately 1743/1720 cm(-1) is tentatively related to a perturbation of the carbonyl modes of phospholipid head groups induced by QH(2) formation. Complex conformational changes of the protein in the amide I and II spectral ranges are also apparent during reduction and reoxidation of the Q pool.
journal_name
FEBS Lettjournal_title
FEBS lettersauthors
Mezzetti A,Leibl W,Breton J,Nabedryk Edoi
10.1016/s0014-5793(03)00118-2keywords:
subject
Has Abstractpub_date
2003-02-27 00:00:00pages
161-5issue
1-3eissn
0014-5793issn
1873-3468pii
S0014579303001182journal_volume
537pub_type
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